Simultaneous Determination of the Main Peanut Allergens in Foods Using Disposable Amperometric Magnetic Beads-Based Immunosensing Platforms

In this work, a novel magnetic beads (MBs)-based immunosensing approach for the rapid and simultaneous determination of the main peanut allergenic proteins (Ara h 1 and Ara h 2) is reported. It involves the use of sandwich-type immunoassays using selective capture and detector antibodies and carboxy...

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Autores: Ruiz Valdepeñas Montiel, Víctor, Torrente Rodríguez, Rebeca Magnolia, Campuzano Ruiz, Susana, Pellicanò, Alessandro, Reviejo García, Ángel Julio, Cosio, Maria, Pingarrón Carrazón, José Manuel
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/23869
Acceso en línea:https://hdl.handle.net/20.500.14352/23869
Access Level:acceso abierto
Palabra clave:Ara h 1
Ara h 2
dual determination
magnetic beads
SPdCEs
amperometric immunosensor
food extracts
Química analítica (Química)
2301 Química Analítica
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spelling Simultaneous Determination of the Main Peanut Allergens in Foods Using Disposable Amperometric Magnetic Beads-Based Immunosensing PlatformsRuiz Valdepeñas Montiel, VíctorTorrente Rodríguez, Rebeca MagnoliaCampuzano Ruiz, SusanaPellicanò, AlessandroReviejo García, Ángel JulioCosio, MariaPingarrón Carrazón, José ManuelAra h 1Ara h 2dual determinationmagnetic beadsSPdCEsamperometric immunosensorfood extractsQuímica analítica (Química)2301 Química AnalíticaIn this work, a novel magnetic beads (MBs)-based immunosensing approach for the rapid and simultaneous determination of the main peanut allergenic proteins (Ara h 1 and Ara h 2) is reported. It involves the use of sandwich-type immunoassays using selective capture and detector antibodies and carboxylic acid-modified magnetic beads (HOOC-MBs). Amperometric detection at −0.20 V was performed using dual screen-printed carbon electrodes (SPdCEs) and the H2O2/hydroquinone (HQ) system. This methodology exhibits high sensitivity and selectivity for the target proteins providing detection limits of 18.0 and 0.07 ng/mL for Ara h 1 and Ara h 2, respectively, with an assay time of only 2 h. The usefulness of the approach was evaluated by detecting the endogenous content of both allergenic proteins in different food extracts as well as trace amounts of peanut allergen (0.0001% or 1.0 mg/kg) in wheat flour spiked samples. The developed platform provides better Low detection limits (LODs) in shorter assay times than those claimed for the allergen specific commercial ELISA kits using the same immunoreagents and quantitative information on individual food allergen levels. Moreover, the flexibility of the methodology makes it readily translate to the detection of other food-allergens.MDPIUniversidad Complutense de Madrid20162016-06-2820162016-06-28journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/23869reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/238692026-06-02T12:44:21Z
dc.title.none.fl_str_mv Simultaneous Determination of the Main Peanut Allergens in Foods Using Disposable Amperometric Magnetic Beads-Based Immunosensing Platforms
title Simultaneous Determination of the Main Peanut Allergens in Foods Using Disposable Amperometric Magnetic Beads-Based Immunosensing Platforms
spellingShingle Simultaneous Determination of the Main Peanut Allergens in Foods Using Disposable Amperometric Magnetic Beads-Based Immunosensing Platforms
Ruiz Valdepeñas Montiel, Víctor
Ara h 1
Ara h 2
dual determination
magnetic beads
SPdCEs
amperometric immunosensor
food extracts
Química analítica (Química)
2301 Química Analítica
title_short Simultaneous Determination of the Main Peanut Allergens in Foods Using Disposable Amperometric Magnetic Beads-Based Immunosensing Platforms
title_full Simultaneous Determination of the Main Peanut Allergens in Foods Using Disposable Amperometric Magnetic Beads-Based Immunosensing Platforms
title_fullStr Simultaneous Determination of the Main Peanut Allergens in Foods Using Disposable Amperometric Magnetic Beads-Based Immunosensing Platforms
title_full_unstemmed Simultaneous Determination of the Main Peanut Allergens in Foods Using Disposable Amperometric Magnetic Beads-Based Immunosensing Platforms
title_sort Simultaneous Determination of the Main Peanut Allergens in Foods Using Disposable Amperometric Magnetic Beads-Based Immunosensing Platforms
dc.creator.none.fl_str_mv Ruiz Valdepeñas Montiel, Víctor
Torrente Rodríguez, Rebeca Magnolia
Campuzano Ruiz, Susana
Pellicanò, Alessandro
Reviejo García, Ángel Julio
Cosio, Maria
Pingarrón Carrazón, José Manuel
author Ruiz Valdepeñas Montiel, Víctor
author_facet Ruiz Valdepeñas Montiel, Víctor
Torrente Rodríguez, Rebeca Magnolia
Campuzano Ruiz, Susana
Pellicanò, Alessandro
Reviejo García, Ángel Julio
Cosio, Maria
Pingarrón Carrazón, José Manuel
author_role author
author2 Torrente Rodríguez, Rebeca Magnolia
Campuzano Ruiz, Susana
Pellicanò, Alessandro
Reviejo García, Ángel Julio
Cosio, Maria
Pingarrón Carrazón, José Manuel
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv Ara h 1
Ara h 2
dual determination
magnetic beads
SPdCEs
amperometric immunosensor
food extracts
Química analítica (Química)
2301 Química Analítica
topic Ara h 1
Ara h 2
dual determination
magnetic beads
SPdCEs
amperometric immunosensor
food extracts
Química analítica (Química)
2301 Química Analítica
description In this work, a novel magnetic beads (MBs)-based immunosensing approach for the rapid and simultaneous determination of the main peanut allergenic proteins (Ara h 1 and Ara h 2) is reported. It involves the use of sandwich-type immunoassays using selective capture and detector antibodies and carboxylic acid-modified magnetic beads (HOOC-MBs). Amperometric detection at −0.20 V was performed using dual screen-printed carbon electrodes (SPdCEs) and the H2O2/hydroquinone (HQ) system. This methodology exhibits high sensitivity and selectivity for the target proteins providing detection limits of 18.0 and 0.07 ng/mL for Ara h 1 and Ara h 2, respectively, with an assay time of only 2 h. The usefulness of the approach was evaluated by detecting the endogenous content of both allergenic proteins in different food extracts as well as trace amounts of peanut allergen (0.0001% or 1.0 mg/kg) in wheat flour spiked samples. The developed platform provides better Low detection limits (LODs) in shorter assay times than those claimed for the allergen specific commercial ELISA kits using the same immunoreagents and quantitative information on individual food allergen levels. Moreover, the flexibility of the methodology makes it readily translate to the detection of other food-allergens.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-06-28
2016
2016-06-28
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/23869
url https://hdl.handle.net/20.500.14352/23869
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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