Isolation and characterization of myogenic precursor cells from human cremaster muscle

Human myogenic precursor cells have been isolated and expanded from a number of skeletal muscles, but alternative donor biopsy sites must be sought after in diseases where muscle damage is widespread. Biopsy sites must be relatively accessible, and the biopsied muscle dispensable. Here, we aimed to...

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Autores: Naldaiz-Gastesi, Neia, Goicoechea, María, Aragón, Isabel Maria, Pérez-López, Virginia, Fuertes-Alvarez, Sandra, Herrera-Imbroda, Bernardo, López de Munain, Adolfo, de Luna-Diaz, Resi, Baptista, Pedro M, Fernández, M Alejandro, Lara, María Fernanda, Izeta, Ander
Formato: artículo
Fecha de publicación:2019
País:España
Recursos:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/7845
Acesso em linha:http://hdl.handle.net/20.500.12105/7845
Access Level:acceso abierto
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spelling Isolation and characterization of myogenic precursor cells from human cremaster muscleNaldaiz-Gastesi, NeiaGoicoechea, MaríaAragón, Isabel MariaPérez-López, VirginiaFuertes-Alvarez, SandraHerrera-Imbroda, BernardoLópez de Munain, Adolfode Luna-Diaz, ResiBaptista, Pedro MFernández, M AlejandroLara, María FernandaIzeta, AnderHuman myogenic precursor cells have been isolated and expanded from a number of skeletal muscles, but alternative donor biopsy sites must be sought after in diseases where muscle damage is widespread. Biopsy sites must be relatively accessible, and the biopsied muscle dispensable. Here, we aimed to histologically characterize the cremaster muscle with regard number of satellite cells and regenerative fibres, and to isolate and characterize human cremaster muscle-derived stem/precursor cells in adult male donors with the objective of characterizing this muscle as a novel source of myogenic precursor cells. Cremaster muscle biopsies (or adjacent non-muscle tissue for negative controls; N = 19) were taken from male patients undergoing routine surgery for urogenital pathology. Myosphere cultures were derived and tested for their in vitro and in vivo myogenic differentiation and muscle regeneration capacities. Cremaster-derived myogenic precursor cells were maintained by myosphere culture and efficiently differentiated to myotubes in adhesion culture. Upon transplantation to an immunocompromised mouse model of cardiotoxin-induced acute muscle damage, human cremaster-derived myogenic precursor cells survived to the transplants and contributed to muscle regeneration. These precursors are a good candidate for cell therapy approaches of skeletal muscle. Due to their location and developmental origin, we propose that they might be best suited for regeneration of the rhabdosphincter in patients undergoing stress urinary incontinence after radical prostatectomy.Nature Publishing GroupMinisterio de Economía y Competitividad (España)Instituto de Salud Carlos IIIUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)Basque Government (España)Regional Government of Andalusia (España)20192019-07-0320192019-03-0520192019-03-05journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12105/7845reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)InglésengES RTC-2015-3750-1 Not availableES PEJ-2014-P-01215 Not availableES FJCI-2016-28121 Not availableES PI13 02172ES PI16 01430ES PRE2013-1-1168 Not availableES PI17 01841ES PI14 00436ES 2015 11038ES RIS3 2017222021 Not availableES BIO16 ERES PI 0222-2014 Not availableopen accesshttp://purl.org/coar/access_right/c_abf2Atribución-NoComercial-CompartirIgual 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/78452026-06-12T12:43:37Z
dc.title.none.fl_str_mv Isolation and characterization of myogenic precursor cells from human cremaster muscle
title Isolation and characterization of myogenic precursor cells from human cremaster muscle
spellingShingle Isolation and characterization of myogenic precursor cells from human cremaster muscle
Naldaiz-Gastesi, Neia
title_short Isolation and characterization of myogenic precursor cells from human cremaster muscle
title_full Isolation and characterization of myogenic precursor cells from human cremaster muscle
title_fullStr Isolation and characterization of myogenic precursor cells from human cremaster muscle
title_full_unstemmed Isolation and characterization of myogenic precursor cells from human cremaster muscle
title_sort Isolation and characterization of myogenic precursor cells from human cremaster muscle
dc.creator.none.fl_str_mv Naldaiz-Gastesi, Neia
Goicoechea, María
Aragón, Isabel Maria
Pérez-López, Virginia
Fuertes-Alvarez, Sandra
Herrera-Imbroda, Bernardo
López de Munain, Adolfo
de Luna-Diaz, Resi
Baptista, Pedro M
Fernández, M Alejandro
Lara, María Fernanda
Izeta, Ander
author Naldaiz-Gastesi, Neia
author_facet Naldaiz-Gastesi, Neia
Goicoechea, María
Aragón, Isabel Maria
Pérez-López, Virginia
Fuertes-Alvarez, Sandra
Herrera-Imbroda, Bernardo
López de Munain, Adolfo
de Luna-Diaz, Resi
Baptista, Pedro M
Fernández, M Alejandro
Lara, María Fernanda
Izeta, Ander
author_role author
author2 Goicoechea, María
Aragón, Isabel Maria
Pérez-López, Virginia
Fuertes-Alvarez, Sandra
Herrera-Imbroda, Bernardo
López de Munain, Adolfo
de Luna-Diaz, Resi
Baptista, Pedro M
Fernández, M Alejandro
Lara, María Fernanda
Izeta, Ander
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Instituto de Salud Carlos III
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
Basque Government (España)
Regional Government of Andalusia (España)
description Human myogenic precursor cells have been isolated and expanded from a number of skeletal muscles, but alternative donor biopsy sites must be sought after in diseases where muscle damage is widespread. Biopsy sites must be relatively accessible, and the biopsied muscle dispensable. Here, we aimed to histologically characterize the cremaster muscle with regard number of satellite cells and regenerative fibres, and to isolate and characterize human cremaster muscle-derived stem/precursor cells in adult male donors with the objective of characterizing this muscle as a novel source of myogenic precursor cells. Cremaster muscle biopsies (or adjacent non-muscle tissue for negative controls; N = 19) were taken from male patients undergoing routine surgery for urogenital pathology. Myosphere cultures were derived and tested for their in vitro and in vivo myogenic differentiation and muscle regeneration capacities. Cremaster-derived myogenic precursor cells were maintained by myosphere culture and efficiently differentiated to myotubes in adhesion culture. Upon transplantation to an immunocompromised mouse model of cardiotoxin-induced acute muscle damage, human cremaster-derived myogenic precursor cells survived to the transplants and contributed to muscle regeneration. These precursors are a good candidate for cell therapy approaches of skeletal muscle. Due to their location and developmental origin, we propose that they might be best suited for regeneration of the rhabdosphincter in patients undergoing stress urinary incontinence after radical prostatectomy.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-07-03
2019
2019-03-05
2019
2019-03-05
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/7845
url http://hdl.handle.net/20.500.12105/7845
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv ES RTC-2015-3750-1 Not available
ES PEJ-2014-P-01215 Not available
ES FJCI-2016-28121 Not available
ES PI13 02172
ES PI16 01430
ES PRE2013-1-1168 Not available
ES PI17 01841
ES PI14 00436
ES 2015 11038
ES RIS3 2017222021 Not available
ES BIO16 ER
ES PI 0222-2014 Not available
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-CompartirIgual 4.0 Internacional
http://creativecommons.org/licenses/by-nc-sa/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-CompartirIgual 4.0 Internacional
http://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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