The FRZB gene regulates hair follicle development in rabbits via the Wnt/B-catenin signaling pathway

[EN] To explore the mechanism of the FRZB gene in hair follicle development by regulating the Wnt/β-catenin signalling pathway, Angora rabbits were selected to collect back skin samples for the experiment. The action mechanism is understood by cell culture and transfection, apoptosis and proliferati...

Descripción completa

Detalles Bibliográficos
Autores: Wang, Fan, Zhang, Xiyu, Dai, Yingying, Zhao, Bohao, Wu, Xinsheng, Chen, Yang
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/199238
Acceso en línea:https://riunet.upv.es/handle/10251/199238
Access Level:acceso abierto
Palabra clave:FRZB gene
Hair follicle
Long-haired rabbit
id ES_3d9dfad551a09efd6ea7c2aeb08a3ae9
oai_identifier_str oai:riunet.upv.es:10251/199238
network_acronym_str ES
network_name_str España
repository_id_str
spelling The FRZB gene regulates hair follicle development in rabbits via the Wnt/B-catenin signaling pathwayWang, FanZhang, XiyuDai, YingyingZhao, BohaoWu, XinshengChen, YangFRZB geneHair follicleLong-haired rabbit[EN] To explore the mechanism of the FRZB gene in hair follicle development by regulating the Wnt/β-catenin signalling pathway, Angora rabbits were selected to collect back skin samples for the experiment. The action mechanism is understood by cell culture and transfection, apoptosis and proliferation assays and TOP/FOP Flash Wnt Reporting System methods. The results showed that the interference and overexpression of the FRZB gene in rabbit dermal papilla cells indicated that overexpression could inhibit the expression of SFRP2, BMP4, and WNT2 genes (P<0.05). On the contrary, the expression of Wnt signalling pathway-related genes LEF1, CCND1, DKK1, and TCF7 was significantly up-regulated (P<0.05). Further examination of the luciferase reporter system TOP/FOP revealed that pcDNA3.1-FRZB inhibits Wnt activity. PcDNA3.1-FRZB was found to promote the level of apoptosis in DP cells, whereas si-FRZB inhibited DP cell proliferation. Therefore, it is concluded that FRZB inhibits hair follicle development in long-haired rabbits by regulating the Wnt/β-catenin signalling pathway.Our study was funded by the National Natural Science Foundation of China (Grant No. 32072724), the Modern Agricultural Industrial System Special Funding (CARS-43-A-1) and the Zhejiang Science and Technology Major Programme on Agricultural New Variety Breeding (2021C02068-7).Universitat Politècnica de ValènciaNational Natural Science Foundation of ChinaScience and Technology Department of Zhejiang ProvinceModern Agricultural Technology Industry System of Shandong provinceRepositorio Institucional de la Universitat Politècnica de València Riunet20232023-09-28journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/199238reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengNational Natural Science Foundation of China https://doi.org/10.13039/501100001809Science and Technology Department of Zhejiang Province Science and Technology Department of Zhejiang ProvinceModern Agricultural Technology Industry System of Shandong province Modern Agricultural Technology Industry System of Shandong provinceopen accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento - No comercial - Compartir igual (by-nc-sa) http://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1992382026-06-13T07:49:27Z
dc.title.none.fl_str_mv The FRZB gene regulates hair follicle development in rabbits via the Wnt/B-catenin signaling pathway
title The FRZB gene regulates hair follicle development in rabbits via the Wnt/B-catenin signaling pathway
spellingShingle The FRZB gene regulates hair follicle development in rabbits via the Wnt/B-catenin signaling pathway
Wang, Fan
FRZB gene
Hair follicle
Long-haired rabbit
title_short The FRZB gene regulates hair follicle development in rabbits via the Wnt/B-catenin signaling pathway
title_full The FRZB gene regulates hair follicle development in rabbits via the Wnt/B-catenin signaling pathway
title_fullStr The FRZB gene regulates hair follicle development in rabbits via the Wnt/B-catenin signaling pathway
title_full_unstemmed The FRZB gene regulates hair follicle development in rabbits via the Wnt/B-catenin signaling pathway
title_sort The FRZB gene regulates hair follicle development in rabbits via the Wnt/B-catenin signaling pathway
dc.creator.none.fl_str_mv Wang, Fan
Zhang, Xiyu
Dai, Yingying
Zhao, Bohao
Wu, Xinsheng
Chen, Yang
author Wang, Fan
author_facet Wang, Fan
Zhang, Xiyu
Dai, Yingying
Zhao, Bohao
Wu, Xinsheng
Chen, Yang
author_role author
author2 Zhang, Xiyu
Dai, Yingying
Zhao, Bohao
Wu, Xinsheng
Chen, Yang
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv National Natural Science Foundation of China
Science and Technology Department of Zhejiang Province
Modern Agricultural Technology Industry System of Shandong province
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv FRZB gene
Hair follicle
Long-haired rabbit
topic FRZB gene
Hair follicle
Long-haired rabbit
description [EN] To explore the mechanism of the FRZB gene in hair follicle development by regulating the Wnt/β-catenin signalling pathway, Angora rabbits were selected to collect back skin samples for the experiment. The action mechanism is understood by cell culture and transfection, apoptosis and proliferation assays and TOP/FOP Flash Wnt Reporting System methods. The results showed that the interference and overexpression of the FRZB gene in rabbit dermal papilla cells indicated that overexpression could inhibit the expression of SFRP2, BMP4, and WNT2 genes (P<0.05). On the contrary, the expression of Wnt signalling pathway-related genes LEF1, CCND1, DKK1, and TCF7 was significantly up-regulated (P<0.05). Further examination of the luciferase reporter system TOP/FOP revealed that pcDNA3.1-FRZB inhibits Wnt activity. PcDNA3.1-FRZB was found to promote the level of apoptosis in DP cells, whereas si-FRZB inhibited DP cell proliferation. Therefore, it is concluded that FRZB inhibits hair follicle development in long-haired rabbits by regulating the Wnt/β-catenin signalling pathway.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-09-28
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/199238
url https://riunet.upv.es/handle/10251/199238
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv National Natural Science Foundation of China https://doi.org/10.13039/501100001809
Science and Technology Department of Zhejiang Province Science and Technology Department of Zhejiang Province
Modern Agricultural Technology Industry System of Shandong province Modern Agricultural Technology Industry System of Shandong province
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento - No comercial - Compartir igual (by-nc-sa)
http://creativecommons.org/licenses/by-nc-sa/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento - No comercial - Compartir igual (by-nc-sa)
http://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universitat Politècnica de València
publisher.none.fl_str_mv Universitat Politècnica de València
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869406458429308928
score 15,300719

Similares en LA Referencia