Changes in leukocytes gene expression profiles induced by antineoplastic chemotherapy

In the present study, we studied changes in gene expression induced by chemotherapy (CT) on normal peripheral blood leukocytes (PBLs), at baseline and following three CT cycles, in order to identify which genes were specifically affected and were potentially useful as biomarkers for a personalised p...

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Detalhes bibliográficos
Autores: Morales González, Manuel José, González-Fernández, Rebeca, Ávila, Julio, Martín-Vasallo, Pablo
Formato: artículo
Fecha de publicación:2012
País:España
Recursos:Universidad de La Laguna (ULL)
Repositorio:RIULL. Repositorio Institucional de la Universidad de La Laguna
OAI Identifier:oai:riull.ull.es:915/40042
Acesso em linha:http://riull.ull.es/xmlui/handle/915/40042
Access Level:acceso abierto
Palavra-chave:Leukocyte
Gene expression
Chemotherapy
Paclitaxel
Carboplatin
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spelling Changes in leukocytes gene expression profiles induced by antineoplastic chemotherapyMorales González, Manuel JoséGonzález-Fernández, RebecaÁvila, JulioMartín-Vasallo, PabloLeukocyteGene expressionChemotherapyPaclitaxelCarboplatinIn the present study, we studied changes in gene expression induced by chemotherapy (CT) on normal peripheral blood leukocytes (PBLs), at baseline and following three CT cycles, in order to identify which genes were specifically affected and were potentially useful as biomarkers for a personalised prognosis and follow-up. A PBL subtraction cDNA library was constructed from four patients undergoing CT with paclitaxel and carboplatin (PC). mRNA from the PBLs was isolated prior to the patients receiving the first cycle and following the completion of the third cycle. The library was screened and the expression of the identified genes was studied in PBLs obtained from patients suffering from cancer prior to and following three cycles of PC and a reference group of patients undergoing treatment with Adriamycincyclophosphamide (AC). From the 1,200 screened colonies, 65 positive clones showed varied expression intensity and were sequenced; 27 of these were mitochondrial DNA and 38 clones (27 different) were coded for cytosolic and nuclear proteins. The genes that were studied in patients undergoing CT were ATM (ataxia-telangiectasia mutated gene), eIF4B (translation initiation factor 4B), MATR3 (Matrin 3), MORC3 (microrchidia 3), PCMTD2 (protein-Lisoaspartate O-methyltransferase), PDCD10 (programmed cell death gene 10), PSMB1 (proteasome subunit type β), RMND5A (required for meiotic nuclear division 5 homologue A), RUNX2 (runt-related transcription factor 2), SACM1L (suppressor of actin mutations 1-like), TMEM66 (transmembrane protein 66) and ZNF644 (zinc finger protein 644). Certain variations were observed in the expression of the genes that are involved in drug resistance mechanisms, some of which may be secondary to non-desirable effects and others of which may cause the undesired effects of CT. The expression of genes with a dynamic cellular role showed a marked positive correlation, indicating that their upregulation may be involved in a specific pattern of cell survival versus apoptosis in response to the cell damage induced by CT. Whether these CT-induced changes are random or directed in a specific selection-evolution manner needs to be elucidated.Medicina Interna, Dermatología y Psiquiatría202420242012info:eu-repo/semantics/articleapplication/pdfhttp://riull.ull.es/xmlui/handle/915/40042reponame:RIULL. Repositorio Institucional de la Universidad de La Lagunainstname:Universidad de La Laguna (ULL)InglésOncology Letters v.3 n.6, 2012Licencia Creative Commons (Reconocimiento-No comercial-Sin obras derivadas 4.0 Internacional)info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es_ESoai:riull.ull.es:915/400422026-06-22T13:13:57Z
dc.title.none.fl_str_mv Changes in leukocytes gene expression profiles induced by antineoplastic chemotherapy
title Changes in leukocytes gene expression profiles induced by antineoplastic chemotherapy
spellingShingle Changes in leukocytes gene expression profiles induced by antineoplastic chemotherapy
Morales González, Manuel José
Leukocyte
Gene expression
Chemotherapy
Paclitaxel
Carboplatin
title_short Changes in leukocytes gene expression profiles induced by antineoplastic chemotherapy
title_full Changes in leukocytes gene expression profiles induced by antineoplastic chemotherapy
title_fullStr Changes in leukocytes gene expression profiles induced by antineoplastic chemotherapy
title_full_unstemmed Changes in leukocytes gene expression profiles induced by antineoplastic chemotherapy
title_sort Changes in leukocytes gene expression profiles induced by antineoplastic chemotherapy
dc.creator.none.fl_str_mv Morales González, Manuel José
González-Fernández, Rebeca
Ávila, Julio
Martín-Vasallo, Pablo
author Morales González, Manuel José
author_facet Morales González, Manuel José
González-Fernández, Rebeca
Ávila, Julio
Martín-Vasallo, Pablo
author_role author
author2 González-Fernández, Rebeca
Ávila, Julio
Martín-Vasallo, Pablo
author2_role author
author
author
dc.contributor.none.fl_str_mv Medicina Interna, Dermatología y Psiquiatría
dc.subject.none.fl_str_mv Leukocyte
Gene expression
Chemotherapy
Paclitaxel
Carboplatin
topic Leukocyte
Gene expression
Chemotherapy
Paclitaxel
Carboplatin
description In the present study, we studied changes in gene expression induced by chemotherapy (CT) on normal peripheral blood leukocytes (PBLs), at baseline and following three CT cycles, in order to identify which genes were specifically affected and were potentially useful as biomarkers for a personalised prognosis and follow-up. A PBL subtraction cDNA library was constructed from four patients undergoing CT with paclitaxel and carboplatin (PC). mRNA from the PBLs was isolated prior to the patients receiving the first cycle and following the completion of the third cycle. The library was screened and the expression of the identified genes was studied in PBLs obtained from patients suffering from cancer prior to and following three cycles of PC and a reference group of patients undergoing treatment with Adriamycincyclophosphamide (AC). From the 1,200 screened colonies, 65 positive clones showed varied expression intensity and were sequenced; 27 of these were mitochondrial DNA and 38 clones (27 different) were coded for cytosolic and nuclear proteins. The genes that were studied in patients undergoing CT were ATM (ataxia-telangiectasia mutated gene), eIF4B (translation initiation factor 4B), MATR3 (Matrin 3), MORC3 (microrchidia 3), PCMTD2 (protein-Lisoaspartate O-methyltransferase), PDCD10 (programmed cell death gene 10), PSMB1 (proteasome subunit type β), RMND5A (required for meiotic nuclear division 5 homologue A), RUNX2 (runt-related transcription factor 2), SACM1L (suppressor of actin mutations 1-like), TMEM66 (transmembrane protein 66) and ZNF644 (zinc finger protein 644). Certain variations were observed in the expression of the genes that are involved in drug resistance mechanisms, some of which may be secondary to non-desirable effects and others of which may cause the undesired effects of CT. The expression of genes with a dynamic cellular role showed a marked positive correlation, indicating that their upregulation may be involved in a specific pattern of cell survival versus apoptosis in response to the cell damage induced by CT. Whether these CT-induced changes are random or directed in a specific selection-evolution manner needs to be elucidated.
publishDate 2012
dc.date.none.fl_str_mv 2012
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://riull.ull.es/xmlui/handle/915/40042
url http://riull.ull.es/xmlui/handle/915/40042
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Oncology Letters v.3 n.6, 2012
dc.rights.none.fl_str_mv Licencia Creative Commons (Reconocimiento-No comercial-Sin obras derivadas 4.0 Internacional)
info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es_ES
rights_invalid_str_mv Licencia Creative Commons (Reconocimiento-No comercial-Sin obras derivadas 4.0 Internacional)
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es_ES
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:RIULL. Repositorio Institucional de la Universidad de La Laguna
instname:Universidad de La Laguna (ULL)
instname_str Universidad de La Laguna (ULL)
reponame_str RIULL. Repositorio Institucional de la Universidad de La Laguna
collection RIULL. Repositorio Institucional de la Universidad de La Laguna
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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