Cooling rate modifies the location of aquaporin 3 in spermatozoa of sheep and goat
30 Pág.
| Autores: | , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/354526 |
| Acceso en línea: | http://hdl.handle.net/10261/354526 |
| Access Level: | acceso abierto |
| Palabra clave: | Aquaporin Domestic ruminants Sperm Cryoresistance |
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Cooling rate modifies the location of aquaporin 3 in spermatozoa of sheep and goatPequeño, BelénMillán de la Blanca, María GemmaCastaño, CristinaToledano-Díaz, AdolfoEsteso, Milagros C.Alba, EstherArrebola, Francisco A.Ungerfeld, RodolfoMartínez-Madrid, BelénÁlvarez-Rodríguez, ManuelRodriguez-Martinez, HeribertoSantiago-Moreno, JuliánAquaporinDomestic ruminantsSpermCryoresistance30 Pág.The freeze-thawing process induces osmotic changes that may affect the membrane domain location of aquaporins’ (AQP) in spermatozoa. Recent studies suggest that changes in AQP3 localization allows better sperm osmo-adaptation, improving the cryoresistance. Ultra-rapid freezing is an alternative cryopreservation technique that requires less equipment than conventional freezing, and it is faster, simpler and can be used in the field. This study aimed to determine the influence of freezing-thawing rates (slow (control) vs. ultra-rapid) on AQP3 expression and location in the spermatozoa from small ruminants (sheep and goats) and its relationship with sperm cryo-damage. Spermatozoa were collected from 10 Merino rams and 10 Murciano-Granadina bucks. The presence and distribution of AQP3 were assessed by Western blotting and immunocytochemistry (ICC), employing a commercial rabbit polyclonal antibody. Sperm motility was CASA system-analyzed, and membrane and acrosome integrity assessed by fluorescence (PI/PNA-FITC). Western blotting did not detect a significant effect of freezing-thawing rate on the amount of AQP3 while ICC found freezing-thawing rate affecting AQP3 location (P<0.05). In both species, the percentages of spermatozoa showing AQP3 in the post-acrosome region, mid-piece, and principal piece of the tail were greater in samples cryopreserved by slow freezing-thawing (control) than ultra-rapid freezing-thawing rates (P<0.05). Spermatozoa cryopreserved using ultra-rapid freezing-thawing showed decrease motility, plasma membrane, and acrosome integrity (P<0.05), which might be related, at least in part, to a lower expression of AQP3. In conclusion, the cooling rate modifies the location of AQP3 in spermatozoa of sheep and goat, which might be associated with sperm cryosurvival.This study was supported by the project PID2020-113288RB-I00/AEI/10.13039/501100011033. B. Pequeño was the recipient of a grant for pre-doctoral researchers from AEI (PRE2018-085637).Peer reviewedElsevierAgencia Estatal de Investigación (España)Pequeño, Belén [0000-0003-3962-1982]Millán de la Blanca, María Gemma [0000-0002-2810-8510]Castaño, Cristina [0000-0003-1134-1436]Toledano-Díaz, A. [0000-0001-6679-485X]Esteso, Milagros C. [0000-0002-8963-5736]Arrebola, Francisco A. [0000-0002-5571-7258]Ungerfeld, Rodolfo [0000-0003-4685-2105]Martínez-Madrid, Belén [0000-0001-6852-4597]Álvarez-Rodríguez, Manuel [0000-0003-0120-354X]Rodriguez-Martinez, Heriberto [0000-0002-5194-2124]Santiago Moreno, Julián [0000-0001-5551-8120]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttp://hdl.handle.net/10261/354526reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113288RB-I00Departamento de Reproducción Animal https://doi.org/10.1016/j.theriogenology.2024.04.008Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3545262026-05-22T06:33:51Z |
| dc.title.none.fl_str_mv |
Cooling rate modifies the location of aquaporin 3 in spermatozoa of sheep and goat |
| title |
Cooling rate modifies the location of aquaporin 3 in spermatozoa of sheep and goat |
| spellingShingle |
Cooling rate modifies the location of aquaporin 3 in spermatozoa of sheep and goat Pequeño, Belén Aquaporin Domestic ruminants Sperm Cryoresistance |
| title_short |
Cooling rate modifies the location of aquaporin 3 in spermatozoa of sheep and goat |
| title_full |
Cooling rate modifies the location of aquaporin 3 in spermatozoa of sheep and goat |
| title_fullStr |
Cooling rate modifies the location of aquaporin 3 in spermatozoa of sheep and goat |
| title_full_unstemmed |
Cooling rate modifies the location of aquaporin 3 in spermatozoa of sheep and goat |
| title_sort |
Cooling rate modifies the location of aquaporin 3 in spermatozoa of sheep and goat |
| dc.creator.none.fl_str_mv |
Pequeño, Belén Millán de la Blanca, María Gemma Castaño, Cristina Toledano-Díaz, Adolfo Esteso, Milagros C. Alba, Esther Arrebola, Francisco A. Ungerfeld, Rodolfo Martínez-Madrid, Belén Álvarez-Rodríguez, Manuel Rodriguez-Martinez, Heriberto Santiago-Moreno, Julián |
| author |
Pequeño, Belén |
| author_facet |
Pequeño, Belén Millán de la Blanca, María Gemma Castaño, Cristina Toledano-Díaz, Adolfo Esteso, Milagros C. Alba, Esther Arrebola, Francisco A. Ungerfeld, Rodolfo Martínez-Madrid, Belén Álvarez-Rodríguez, Manuel Rodriguez-Martinez, Heriberto Santiago-Moreno, Julián |
| author_role |
author |
| author2 |
Millán de la Blanca, María Gemma Castaño, Cristina Toledano-Díaz, Adolfo Esteso, Milagros C. Alba, Esther Arrebola, Francisco A. Ungerfeld, Rodolfo Martínez-Madrid, Belén Álvarez-Rodríguez, Manuel Rodriguez-Martinez, Heriberto Santiago-Moreno, Julián |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Agencia Estatal de Investigación (España) Pequeño, Belén [0000-0003-3962-1982] Millán de la Blanca, María Gemma [0000-0002-2810-8510] Castaño, Cristina [0000-0003-1134-1436] Toledano-Díaz, A. [0000-0001-6679-485X] Esteso, Milagros C. [0000-0002-8963-5736] Arrebola, Francisco A. [0000-0002-5571-7258] Ungerfeld, Rodolfo [0000-0003-4685-2105] Martínez-Madrid, Belén [0000-0001-6852-4597] Álvarez-Rodríguez, Manuel [0000-0003-0120-354X] Rodriguez-Martinez, Heriberto [0000-0002-5194-2124] Santiago Moreno, Julián [0000-0001-5551-8120] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
Aquaporin Domestic ruminants Sperm Cryoresistance |
| topic |
Aquaporin Domestic ruminants Sperm Cryoresistance |
| description |
30 Pág. |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2024 2024 2024 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Postprint info:eu-repo/semantics/acceptedVersion |
| format |
article |
| status_str |
acceptedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10261/354526 |
| url |
http://hdl.handle.net/10261/354526 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
#PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113288RB-I00 Departamento de Reproducción Animal https://doi.org/10.1016/j.theriogenology.2024.04.008 Sí |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Elsevier |
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Elsevier |
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reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Consejo Superior de Investigaciones Científicas (CSIC) |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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15,812429 |