CB1 knockout mice display impaired functionality of 5-HT1A and 5-HT2A/C receptors
Interaction between brain endocannabinoid (EC) and serotonin (5-HT) systems was investigated by examining 5-HT-dependent behavioural and biochemical responses in CB1 receptor knockout mice. CB1 knockout animals exhibited a significant reduction in the induction of head twitches and paw tremor by the...
| Autores: | , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión enviada para evaluación y publicación |
| Fecha de publicación: | 2007 |
| País: | España |
| Institución: | Universitat Pompeu Fabra |
| Repositorio: | Repositorio Digital de la UPF |
| OAI Identifier: | oai:repositori.upf.edu:10230/12418 |
| Acceso en línea: | http://hdl.handle.net/10230/12418 http://dx.doi.org/10.1111/j.1471-4159.2007.04961.x |
| Access Level: | acceso abierto |
| Palabra clave: | Serotonina Cannabinoides -- Receptors Cannabinoides -- Efectes fisiològics CB1 receptors 5-HT1A receptors 5-HT2A receptors Antidepressants |
| id |
ES_3d77faeb7ab0baa0dc21624a47cfaf35 |
|---|---|
| oai_identifier_str |
oai:repositori.upf.edu:10230/12418 |
| network_acronym_str |
ES |
| network_name_str |
España |
| repository_id_str |
|
| spelling |
CB1 knockout mice display impaired functionality of 5-HT1A and 5-HT2A/C receptorsMato, SusanaAso Pérez, EsterCastro, ElenaMartín, MiguelValverde Granados, OlgaMaldonado, Rafael, 1961-Pazos, ÁngelSerotoninaCannabinoides -- ReceptorsCannabinoides -- Efectes fisiològicsCB1 receptors5-HT1A receptors5-HT2A receptorsAntidepressantsInteraction between brain endocannabinoid (EC) and serotonin (5-HT) systems was investigated by examining 5-HT-dependent behavioural and biochemical responses in CB1 receptor knockout mice. CB1 knockout animals exhibited a significant reduction in the induction of head twitches and paw tremor by the 5-HT2A receptor selective agonist ()DOI, as well as a reduced hypothermic response following administration of the 5-HT1A receptor agonist (±)-8-OH-DPAT. Additionally, exposure to the tail suspension test induced enhanced despair responses in CB1 knockout mice. However, the tricyclic antidepressant imipramine and the 5-HT selective reuptake inhibitor fluoxetine induced similar decreases in the time of immobility in the tail suspension test in CB1 receptor knockout and wild-type mice. No differences were found between both genotypes with regard to 5-HT2A receptor and 5-HT1A receptors levels, measured by autoradiography in different brain areas. However, a significant decrease in the ability of the 5-HT1A receptor agonist (±)-8-OH-DPAT to stimulate 35SGTPS binding was detected in the hippocampal CA1 area of CB1 receptor knockout mice. This study provides evidence that CB1 receptors are involved in the regulation of serotonergic responses mediated by 5-HT2A and 5-HT1A receptors, and suggests that a reduced coupling of 5-HT1A receptors to Gi/o proteins in the hippocampus might be involved in these effects.S. Mato and E. Aso were recipients of predoctoral fellowships from the Marqués de Valdecilla Foundation and the Spanish MCYT (BES-2005-8265), respectively. E. Castro is a fellow researcher under an University of Cantabria- Faes Farma contract. The technical assistance of Ms. Lourdes Lanza and Ms. Josefa Castillo is kindly acknowledged. This study was partially supported by Ministerio de Ciencia y Tecnología (CICYT SAF04-00941 to AP; SAF2004/0568 to OV; BFU2004/00920BFI to RM,and GEN2003-20651-C06-06 to RM), the European Communities (NEWMOOD LSHM-CT-2003 5003474 to RM) and Generalitat de Catalunya (2005SGR00131 to RM)Wiley-Blackwell201120112007info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/12418http://dx.doi.org/10.1111/j.1471-4159.2007.04961.xreponame:Repositorio Digital de la UPFinstname:Universitat Pompeu FabraInglésJournal of Neurochemistry. 2007;103(5):2111-20© 2007 Ester Asp et al. Journal Compilation © 2007 International Society for Neurochemistryinfo:eu-repo/semantics/openAccessoai:repositori.upf.edu:10230/124182026-06-12T07:21:37Z |
| dc.title.none.fl_str_mv |
CB1 knockout mice display impaired functionality of 5-HT1A and 5-HT2A/C receptors |
| title |
CB1 knockout mice display impaired functionality of 5-HT1A and 5-HT2A/C receptors |
| spellingShingle |
CB1 knockout mice display impaired functionality of 5-HT1A and 5-HT2A/C receptors Mato, Susana Serotonina Cannabinoides -- Receptors Cannabinoides -- Efectes fisiològics CB1 receptors 5-HT1A receptors 5-HT2A receptors Antidepressants |
| title_short |
CB1 knockout mice display impaired functionality of 5-HT1A and 5-HT2A/C receptors |
| title_full |
CB1 knockout mice display impaired functionality of 5-HT1A and 5-HT2A/C receptors |
| title_fullStr |
CB1 knockout mice display impaired functionality of 5-HT1A and 5-HT2A/C receptors |
| title_full_unstemmed |
CB1 knockout mice display impaired functionality of 5-HT1A and 5-HT2A/C receptors |
| title_sort |
CB1 knockout mice display impaired functionality of 5-HT1A and 5-HT2A/C receptors |
| dc.creator.none.fl_str_mv |
Mato, Susana Aso Pérez, Ester Castro, Elena Martín, Miguel Valverde Granados, Olga Maldonado, Rafael, 1961- Pazos, Ángel |
| author |
Mato, Susana |
| author_facet |
Mato, Susana Aso Pérez, Ester Castro, Elena Martín, Miguel Valverde Granados, Olga Maldonado, Rafael, 1961- Pazos, Ángel |
| author_role |
author |
| author2 |
Aso Pérez, Ester Castro, Elena Martín, Miguel Valverde Granados, Olga Maldonado, Rafael, 1961- Pazos, Ángel |
| author2_role |
author author author author author author |
| dc.subject.none.fl_str_mv |
Serotonina Cannabinoides -- Receptors Cannabinoides -- Efectes fisiològics CB1 receptors 5-HT1A receptors 5-HT2A receptors Antidepressants |
| topic |
Serotonina Cannabinoides -- Receptors Cannabinoides -- Efectes fisiològics CB1 receptors 5-HT1A receptors 5-HT2A receptors Antidepressants |
| description |
Interaction between brain endocannabinoid (EC) and serotonin (5-HT) systems was investigated by examining 5-HT-dependent behavioural and biochemical responses in CB1 receptor knockout mice. CB1 knockout animals exhibited a significant reduction in the induction of head twitches and paw tremor by the 5-HT2A receptor selective agonist ()DOI, as well as a reduced hypothermic response following administration of the 5-HT1A receptor agonist (±)-8-OH-DPAT. Additionally, exposure to the tail suspension test induced enhanced despair responses in CB1 knockout mice. However, the tricyclic antidepressant imipramine and the 5-HT selective reuptake inhibitor fluoxetine induced similar decreases in the time of immobility in the tail suspension test in CB1 receptor knockout and wild-type mice. No differences were found between both genotypes with regard to 5-HT2A receptor and 5-HT1A receptors levels, measured by autoradiography in different brain areas. However, a significant decrease in the ability of the 5-HT1A receptor agonist (±)-8-OH-DPAT to stimulate 35SGTPS binding was detected in the hippocampal CA1 area of CB1 receptor knockout mice. This study provides evidence that CB1 receptors are involved in the regulation of serotonergic responses mediated by 5-HT2A and 5-HT1A receptors, and suggests that a reduced coupling of 5-HT1A receptors to Gi/o proteins in the hippocampus might be involved in these effects. |
| publishDate |
2007 |
| dc.date.none.fl_str_mv |
2007 2011 2011 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/submittedVersion |
| format |
article |
| status_str |
submittedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10230/12418 http://dx.doi.org/10.1111/j.1471-4159.2007.04961.x |
| url |
http://hdl.handle.net/10230/12418 http://dx.doi.org/10.1111/j.1471-4159.2007.04961.x |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Journal of Neurochemistry. 2007;103(5):2111-20 |
| dc.rights.none.fl_str_mv |
© 2007 Ester Asp et al. Journal Compilation © 2007 International Society for Neurochemistry info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
© 2007 Ester Asp et al. Journal Compilation © 2007 International Society for Neurochemistry |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf application/pdf |
| dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
| publisher.none.fl_str_mv |
Wiley-Blackwell |
| dc.source.none.fl_str_mv |
reponame:Repositorio Digital de la UPF instname:Universitat Pompeu Fabra |
| instname_str |
Universitat Pompeu Fabra |
| reponame_str |
Repositorio Digital de la UPF |
| collection |
Repositorio Digital de la UPF |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
|
| _version_ |
1869406442158555136 |
| score |
15,81155 |