Effect of 16h of rabbit sperm incubation on capacitation and heterologous in vitro fertilization

In vitro fertilization (IVF) in rabbits is an important biotechnology, but its success remains low due to inefficient sperm capacitation. This study examines relationships between motility parameters (MP), protein tyrosine phosphorylation (PTP) and acrosomal exocytosis (AE) between sperm recovered a...

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Detalles Bibliográficos
Autores: Ruiz-Díaz, Sara, Maroto, María, Fernández-González, Raúl, Frutos, Celia de, Lombó, Marta, Arias-Álvarez, María
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/394958
Acceso en línea:http://hdl.handle.net/10261/394958
https://api.elsevier.com/content/abstract/scopus_id/105003683727
Access Level:acceso abierto
Palabra clave:Acrosome reaction
Annulus-ring
Assisted reproductive technologies
Protein tyrosine phosphorylation
Rabbit
Sperm capacitation
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spelling Effect of 16h of rabbit sperm incubation on capacitation and heterologous in vitro fertilizationRuiz-Díaz, SaraMaroto, MaríaFernández-González, RaúlFrutos, Celia deLombó, MartaArias-Álvarez, MaríaAcrosome reactionAnnulus-ringAssisted reproductive technologiesProtein tyrosine phosphorylationRabbitSperm capacitationIn vitro fertilization (IVF) in rabbits is an important biotechnology, but its success remains low due to inefficient sperm capacitation. This study examines relationships between motility parameters (MP), protein tyrosine phosphorylation (PTP) and acrosomal exocytosis (AE) between sperm recovered after direct swim-up (DSU), and after 16 h of in vitro incubation under capacitating conditions, and heterologous IVF capacity. Variables were assessed in fresh ejaculates (ES), and after 2 h of DSU, and 16 h (T16) of incubation. PTP were grouped into four patterns in the different sperm structures according to their time-dependent appearance and AE. Throughout incubation, MP remained stable whereas AE increased after DSU and 16 h of incubation. Mid-piece PTP patterns increased after DSU and persisted until 16 h in reacted (R) and non-reacted spermatozoa (NR). In R and NR, annulus-ring PTP levels were also significantly higher in T16 versus DSU or ES. Flagellum PTP levels rose significantly during incubation such that highest levels were recorded in T16. In R sperm, levels of PTP patterns associated with the equatorial zone in T16 were significantly higher compared to NR and to DSU and ES. Cleavage rates were significantly higher at T16 than DSU and these were similar to rates recorded for homologous IVF used as positive control. Our capacitation protocol and PTP patterns at 16 h rendered the sperm competent to fertilize an oocyte in vitro. This time point matches the physiological timing of fertilization in vivo. We also describe a new annulus ring PTP pattern for this species.This research was supported by Grants PID2019–111641RB-I00 and PID2021–122507OB-I00 funded by MCIN/AEI/10.13039/501100011033/ and European Union “NextGenerationEU”/PRTR), RTI2018–094404-B-C22 and PID2022–138223OB-100. M.L. was supported by a Juan de la Cierva postdoctoral contract (FJC2019-040385-I) from the MICINN. S.R.D. was supported by a “Doctorados Industriales 2018” fellowship of Comunidad de Madrid (IND2018/BIO-9610).Peer reviewedElsevier BVMinisterio de Ciencia e Innovación (España)Agencia Estatal de Investigación (España)European CommissionComunidad de MadridConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/394958https://api.elsevier.com/content/abstract/scopus_id/105003683727reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-111641RB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-122507OB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094404-B-C22info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-138223OB-I00S2018/BIO-9610https://doi.org/10.1016/j.anireprosci.2025.107841Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3949582026-05-22T06:33:51Z
dc.title.none.fl_str_mv Effect of 16h of rabbit sperm incubation on capacitation and heterologous in vitro fertilization
title Effect of 16h of rabbit sperm incubation on capacitation and heterologous in vitro fertilization
spellingShingle Effect of 16h of rabbit sperm incubation on capacitation and heterologous in vitro fertilization
Ruiz-Díaz, Sara
Acrosome reaction
Annulus-ring
Assisted reproductive technologies
Protein tyrosine phosphorylation
Rabbit
Sperm capacitation
title_short Effect of 16h of rabbit sperm incubation on capacitation and heterologous in vitro fertilization
title_full Effect of 16h of rabbit sperm incubation on capacitation and heterologous in vitro fertilization
title_fullStr Effect of 16h of rabbit sperm incubation on capacitation and heterologous in vitro fertilization
title_full_unstemmed Effect of 16h of rabbit sperm incubation on capacitation and heterologous in vitro fertilization
title_sort Effect of 16h of rabbit sperm incubation on capacitation and heterologous in vitro fertilization
dc.creator.none.fl_str_mv Ruiz-Díaz, Sara
Maroto, María
Fernández-González, Raúl
Frutos, Celia de
Lombó, Marta
Arias-Álvarez, María
author Ruiz-Díaz, Sara
author_facet Ruiz-Díaz, Sara
Maroto, María
Fernández-González, Raúl
Frutos, Celia de
Lombó, Marta
Arias-Álvarez, María
author_role author
author2 Maroto, María
Fernández-González, Raúl
Frutos, Celia de
Lombó, Marta
Arias-Álvarez, María
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Agencia Estatal de Investigación (España)
European Commission
Comunidad de Madrid
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Acrosome reaction
Annulus-ring
Assisted reproductive technologies
Protein tyrosine phosphorylation
Rabbit
Sperm capacitation
topic Acrosome reaction
Annulus-ring
Assisted reproductive technologies
Protein tyrosine phosphorylation
Rabbit
Sperm capacitation
description In vitro fertilization (IVF) in rabbits is an important biotechnology, but its success remains low due to inefficient sperm capacitation. This study examines relationships between motility parameters (MP), protein tyrosine phosphorylation (PTP) and acrosomal exocytosis (AE) between sperm recovered after direct swim-up (DSU), and after 16 h of in vitro incubation under capacitating conditions, and heterologous IVF capacity. Variables were assessed in fresh ejaculates (ES), and after 2 h of DSU, and 16 h (T16) of incubation. PTP were grouped into four patterns in the different sperm structures according to their time-dependent appearance and AE. Throughout incubation, MP remained stable whereas AE increased after DSU and 16 h of incubation. Mid-piece PTP patterns increased after DSU and persisted until 16 h in reacted (R) and non-reacted spermatozoa (NR). In R and NR, annulus-ring PTP levels were also significantly higher in T16 versus DSU or ES. Flagellum PTP levels rose significantly during incubation such that highest levels were recorded in T16. In R sperm, levels of PTP patterns associated with the equatorial zone in T16 were significantly higher compared to NR and to DSU and ES. Cleavage rates were significantly higher at T16 than DSU and these were similar to rates recorded for homologous IVF used as positive control. Our capacitation protocol and PTP patterns at 16 h rendered the sperm competent to fertilize an oocyte in vitro. This time point matches the physiological timing of fertilization in vivo. We also describe a new annulus ring PTP pattern for this species.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/394958
https://api.elsevier.com/content/abstract/scopus_id/105003683727
url http://hdl.handle.net/10261/394958
https://api.elsevier.com/content/abstract/scopus_id/105003683727
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-111641RB-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-122507OB-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094404-B-C22
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-138223OB-I00
S2018/BIO-9610
https://doi.org/10.1016/j.anireprosci.2025.107841
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier BV
publisher.none.fl_str_mv Elsevier BV
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
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