Determination of atropine and scopolamine in honey using a miniaturized polymer-based solid-phase extraction protocol prior to the analysis by HPLC-MS/MS

Two homopolymers have been prepared employing methacrylic acid and 4-vynylpiridine as functional monomers (p-MAA, p-4-VP) through an easy and quick precipitation polymerization method for application as sorbents in solid-phase extraction (SPE) to determine atropine and scopolamine in honey. The opti...

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Autores: Fernández Pintor, Begoña, Gañán Aceituno, Judith, Morante Zarcero, Sonia, Sierra Alonso, Isabel, Paniagua González, Gema, Fernández Hernando, Pilar, Garcinuño Martínez, Rosa Mª
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universidad Nacional de Educación a Distancia
Repositorio:e-spacio. Repositorio Institucional de la UNED
Idioma:inglés
OAI Identifier:oai:e-spacio.uned.es:20.500.14468/22314
Acceso en línea:https://hdl.handle.net/20.500.14468/22314
Access Level:acceso abierto
Palabra clave:Tropane alkaloids
Atropine
Scopolamine
Honey samples
Solid-phase extraction
HPLC-MS/MS
Polymer
Food safety
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spelling Determination of atropine and scopolamine in honey using a miniaturized polymer-based solid-phase extraction protocol prior to the analysis by HPLC-MS/MSFernández Pintor, BegoñaGañán Aceituno, JudithMorante Zarcero, SoniaSierra Alonso, IsabelPaniagua González, GemaFernández Hernando, PilarGarcinuño Martínez, Rosa MªTropane alkaloidsAtropineScopolamineHoney samplesSolid-phase extractionHPLC-MS/MSPolymerFood safetyTwo homopolymers have been prepared employing methacrylic acid and 4-vynylpiridine as functional monomers (p-MAA, p-4-VP) through an easy and quick precipitation polymerization method for application as sorbents in solid-phase extraction (SPE) to determine atropine and scopolamine in honey. The optimized SPE conditions were as follows: 25 mg of p-MAA, 4 mL of sample loading volume (diluted honey 1:10 with water), 4 mL of elution solvent (methanol/water with 1 % formic acid, 80/20, v/v). The extracts were analyzed by HPLC-MS/MS. The cartridges were reusable for forty cycles demonstrating an environmentally friendly approach. The methodology was validated in terms of linearity, accuracy, precision, selectivity, matrix effect and sensibility, highlighting the absence of matrix effect. The miniaturized polymer-based SPE was successfully applied to fifteen honeys, showing concentrations up to 7.23 ng/g in the most contaminated honey. All quantified honey samples (5 in total) were of multifloral type.Elseviere-Spacio UNED20242024-06-1120242024-04-0920242024-04-09journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14468/22314reponame:e-spacio. Repositorio Institucional de la UNEDinstname:Universidad Nacional de Educación a DistanciaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/4.0/deed.esoai:e-spacio.uned.es:20.500.14468/223142026-06-06T12:38:31Z
dc.title.none.fl_str_mv Determination of atropine and scopolamine in honey using a miniaturized polymer-based solid-phase extraction protocol prior to the analysis by HPLC-MS/MS
title Determination of atropine and scopolamine in honey using a miniaturized polymer-based solid-phase extraction protocol prior to the analysis by HPLC-MS/MS
spellingShingle Determination of atropine and scopolamine in honey using a miniaturized polymer-based solid-phase extraction protocol prior to the analysis by HPLC-MS/MS
Fernández Pintor, Begoña
Tropane alkaloids
Atropine
Scopolamine
Honey samples
Solid-phase extraction
HPLC-MS/MS
Polymer
Food safety
title_short Determination of atropine and scopolamine in honey using a miniaturized polymer-based solid-phase extraction protocol prior to the analysis by HPLC-MS/MS
title_full Determination of atropine and scopolamine in honey using a miniaturized polymer-based solid-phase extraction protocol prior to the analysis by HPLC-MS/MS
title_fullStr Determination of atropine and scopolamine in honey using a miniaturized polymer-based solid-phase extraction protocol prior to the analysis by HPLC-MS/MS
title_full_unstemmed Determination of atropine and scopolamine in honey using a miniaturized polymer-based solid-phase extraction protocol prior to the analysis by HPLC-MS/MS
title_sort Determination of atropine and scopolamine in honey using a miniaturized polymer-based solid-phase extraction protocol prior to the analysis by HPLC-MS/MS
dc.creator.none.fl_str_mv Fernández Pintor, Begoña
Gañán Aceituno, Judith
Morante Zarcero, Sonia
Sierra Alonso, Isabel
Paniagua González, Gema
Fernández Hernando, Pilar
Garcinuño Martínez, Rosa Mª
author Fernández Pintor, Begoña
author_facet Fernández Pintor, Begoña
Gañán Aceituno, Judith
Morante Zarcero, Sonia
Sierra Alonso, Isabel
Paniagua González, Gema
Fernández Hernando, Pilar
Garcinuño Martínez, Rosa Mª
author_role author
author2 Gañán Aceituno, Judith
Morante Zarcero, Sonia
Sierra Alonso, Isabel
Paniagua González, Gema
Fernández Hernando, Pilar
Garcinuño Martínez, Rosa Mª
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv e-Spacio UNED
dc.subject.none.fl_str_mv Tropane alkaloids
Atropine
Scopolamine
Honey samples
Solid-phase extraction
HPLC-MS/MS
Polymer
Food safety
topic Tropane alkaloids
Atropine
Scopolamine
Honey samples
Solid-phase extraction
HPLC-MS/MS
Polymer
Food safety
description Two homopolymers have been prepared employing methacrylic acid and 4-vynylpiridine as functional monomers (p-MAA, p-4-VP) through an easy and quick precipitation polymerization method for application as sorbents in solid-phase extraction (SPE) to determine atropine and scopolamine in honey. The optimized SPE conditions were as follows: 25 mg of p-MAA, 4 mL of sample loading volume (diluted honey 1:10 with water), 4 mL of elution solvent (methanol/water with 1 % formic acid, 80/20, v/v). The extracts were analyzed by HPLC-MS/MS. The cartridges were reusable for forty cycles demonstrating an environmentally friendly approach. The methodology was validated in terms of linearity, accuracy, precision, selectivity, matrix effect and sensibility, highlighting the absence of matrix effect. The miniaturized polymer-based SPE was successfully applied to fifteen honeys, showing concentrations up to 7.23 ng/g in the most contaminated honey. All quantified honey samples (5 in total) were of multifloral type.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-06-11
2024
2024-04-09
2024
2024-04-09
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14468/22314
url https://hdl.handle.net/20.500.14468/22314
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/4.0/deed.es
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc/4.0/deed.es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:e-spacio. Repositorio Institucional de la UNED
instname:Universidad Nacional de Educación a Distancia
instname_str Universidad Nacional de Educación a Distancia
reponame_str e-spacio. Repositorio Institucional de la UNED
collection e-spacio. Repositorio Institucional de la UNED
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,30478

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