Cellular and Molecular Events that Occur in the Oocyte during Prolonged Ovarian Storage in Sheep

[EN] For the past two decades, there has been a growing interest in the application of in vitro embryo production (IVP) in small ruminants such as sheep. To improve efficiency, a large number abattoir-derived ovaries must be used, and long distances from the laboratory are usually inevitable when ad...

Descripción completa

Detalles Bibliográficos
Autores: Martín Maestro, Alicia, Sánchez Ajofrín, Irene, Maside Mielgo, Carolina, Peris Frau, Patricia, Medina Chávez, Daniela Alejandra, Cardoso, Beatriz, Navarro, José Carlos, Fernández Santos, María Rocío, Garde, José Julián, Soler, Ana Josefa
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Universidad de León
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/23602
Acceso en línea:https://www.mdpi.com/2076-2615/10/12/2414
https://hdl.handle.net/10612/23602
Access Level:acceso abierto
Palabra clave:Veterinaria
Sheep
Ovary storage
Transport
Oocyte
In vitro embryo production
id ES_3d4b107220fdb8b7451e7f2bc4e53584
oai_identifier_str oai:buleria.unileon.es:10612/23602
network_acronym_str ES
network_name_str España
repository_id_str
spelling Cellular and Molecular Events that Occur in the Oocyte during Prolonged Ovarian Storage in SheepMartín Maestro, AliciaSánchez Ajofrín, IreneMaside Mielgo, CarolinaPeris Frau, PatriciaMedina Chávez, Daniela AlejandraCardoso, BeatrizNavarro, José CarlosFernández Santos, María RocíoGarde, José JuliánSoler, Ana JosefaVeterinariaSheepOvary storageTransportOocyteIn vitro embryo production[EN] For the past two decades, there has been a growing interest in the application of in vitro embryo production (IVP) in small ruminants such as sheep. To improve efficiency, a large number abattoir-derived ovaries must be used, and long distances from the laboratory are usually inevitable when adult animals are used. In that scenario, prolonged sheep ovary transportation may negatively affect oocyte developmental competence. Here, we evaluated the effect of ovary storage time (3, 5, 7, 9, 11 and 13 h) and the medium in which they were transported (TCM199 and saline solution) on oocyte quality. Thus, live/dead status, early apoptosis, DNA fragmentation, reduced glutathione (GSH) and reactive oxygen species (ROS) content, caspase-3 activity, mitochondrial membrane potential and distribution, and relative abundance of mRNA transcript levels were assessed in oocytes. After in vitro maturation (IVM), cumulus cell viability and quality, meiotic and fertilization competence, embryo rates and blastocyst quality were also evaluated. The results revealed that, after 7 h of storage, oocyte quality and developmental potential were significantly impaired since higher rates of dead oocytes and DNA fragmentation and lower rates of viable, matured and fertilized oocytes were observed. The percentage of cleavage, blastocyst rates and cumulus cell parameters (viability, active mitochondria and GSH/ROS ratio) were also decreased. Moreover, the preservation of ovaries in medium TCM199 had a detrimental effect on cumulus cells and oocyte competence. In conclusion, ovary transport times up to 5 h in saline solution are the most adequate storage conditions to maintain oocyte quality as well as developmental capacity in sheep. A strategy to rescue the poor developmental potential of stored oocytes will be necessary for successful production of high-quality embryos when longer ovarian preservation times are necessary.SISpanish Ministry of Economy and CompetitivenessUniversity of Castilla-La Mancha scholarshipMDPISanidad AnimalFacultad de Veterinaria2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://www.mdpi.com/2076-2615/10/12/2414https://hdl.handle.net/10612/23602reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad de LeónIngléshttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/236022026-06-24T12:43:27Z
dc.title.none.fl_str_mv Cellular and Molecular Events that Occur in the Oocyte during Prolonged Ovarian Storage in Sheep
title Cellular and Molecular Events that Occur in the Oocyte during Prolonged Ovarian Storage in Sheep
spellingShingle Cellular and Molecular Events that Occur in the Oocyte during Prolonged Ovarian Storage in Sheep
Martín Maestro, Alicia
Veterinaria
Sheep
Ovary storage
Transport
Oocyte
In vitro embryo production
title_short Cellular and Molecular Events that Occur in the Oocyte during Prolonged Ovarian Storage in Sheep
title_full Cellular and Molecular Events that Occur in the Oocyte during Prolonged Ovarian Storage in Sheep
title_fullStr Cellular and Molecular Events that Occur in the Oocyte during Prolonged Ovarian Storage in Sheep
title_full_unstemmed Cellular and Molecular Events that Occur in the Oocyte during Prolonged Ovarian Storage in Sheep
title_sort Cellular and Molecular Events that Occur in the Oocyte during Prolonged Ovarian Storage in Sheep
dc.creator.none.fl_str_mv Martín Maestro, Alicia
Sánchez Ajofrín, Irene
Maside Mielgo, Carolina
Peris Frau, Patricia
Medina Chávez, Daniela Alejandra
Cardoso, Beatriz
Navarro, José Carlos
Fernández Santos, María Rocío
Garde, José Julián
Soler, Ana Josefa
author Martín Maestro, Alicia
author_facet Martín Maestro, Alicia
Sánchez Ajofrín, Irene
Maside Mielgo, Carolina
Peris Frau, Patricia
Medina Chávez, Daniela Alejandra
Cardoso, Beatriz
Navarro, José Carlos
Fernández Santos, María Rocío
Garde, José Julián
Soler, Ana Josefa
author_role author
author2 Sánchez Ajofrín, Irene
Maside Mielgo, Carolina
Peris Frau, Patricia
Medina Chávez, Daniela Alejandra
Cardoso, Beatriz
Navarro, José Carlos
Fernández Santos, María Rocío
Garde, José Julián
Soler, Ana Josefa
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Sanidad Animal
Facultad de Veterinaria
dc.subject.none.fl_str_mv Veterinaria
Sheep
Ovary storage
Transport
Oocyte
In vitro embryo production
topic Veterinaria
Sheep
Ovary storage
Transport
Oocyte
In vitro embryo production
description [EN] For the past two decades, there has been a growing interest in the application of in vitro embryo production (IVP) in small ruminants such as sheep. To improve efficiency, a large number abattoir-derived ovaries must be used, and long distances from the laboratory are usually inevitable when adult animals are used. In that scenario, prolonged sheep ovary transportation may negatively affect oocyte developmental competence. Here, we evaluated the effect of ovary storage time (3, 5, 7, 9, 11 and 13 h) and the medium in which they were transported (TCM199 and saline solution) on oocyte quality. Thus, live/dead status, early apoptosis, DNA fragmentation, reduced glutathione (GSH) and reactive oxygen species (ROS) content, caspase-3 activity, mitochondrial membrane potential and distribution, and relative abundance of mRNA transcript levels were assessed in oocytes. After in vitro maturation (IVM), cumulus cell viability and quality, meiotic and fertilization competence, embryo rates and blastocyst quality were also evaluated. The results revealed that, after 7 h of storage, oocyte quality and developmental potential were significantly impaired since higher rates of dead oocytes and DNA fragmentation and lower rates of viable, matured and fertilized oocytes were observed. The percentage of cleavage, blastocyst rates and cumulus cell parameters (viability, active mitochondria and GSH/ROS ratio) were also decreased. Moreover, the preservation of ovaries in medium TCM199 had a detrimental effect on cumulus cells and oocyte competence. In conclusion, ovary transport times up to 5 h in saline solution are the most adequate storage conditions to maintain oocyte quality as well as developmental capacity in sheep. A strategy to rescue the poor developmental potential of stored oocytes will be necessary for successful production of high-quality embryos when longer ovarian preservation times are necessary.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.mdpi.com/2076-2615/10/12/2414
https://hdl.handle.net/10612/23602
url https://www.mdpi.com/2076-2615/10/12/2414
https://hdl.handle.net/10612/23602
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad de León
instname_str Universidad de León
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869406430904188928
score 15,812429

Similares en LA Referencia