Dietary Milk or Isolated Legume Proteins Modulate Intestinal Microbiota Composition in Rats

Shifts toward increased proteolytic fermentation, such as, for example, in athlete and high-protein weight loss diets, may alter the relative abundance of microbial species in the gut and generate bioactive, potentially deleterious metabolic products. In the current investigation, intestinal (caecal...

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Detalhes bibliográficos
Autor: Rubio, Luis A.
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2024
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/341631
Acesso em linha:http://hdl.handle.net/10261/341631
Access Level:Acceso aberto
Palavra-chave:Intestinal microbiota composition
Lactalbumin
Casein
Cicer arietinum
Lupinus angustifiolius
Protein isolates
Rat
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spelling Dietary Milk or Isolated Legume Proteins Modulate Intestinal Microbiota Composition in RatsRubio, Luis A.Intestinal microbiota compositionLactalbuminCaseinCicer arietinumLupinus angustifioliusProtein isolatesRatShifts toward increased proteolytic fermentation, such as, for example, in athlete and high-protein weight loss diets, may alter the relative abundance of microbial species in the gut and generate bioactive, potentially deleterious metabolic products. In the current investigation, intestinal (caecal) microbiota composition was studied in rats fed diets differing only in their constituent proteins: milk (casein (CAS), lactalbumin (LA)) or legume (Cicer arietinum, Lupinus angustifolius) protein isolates (chickpea protein isolate (CPI), lupin protein isolate (LI)). ANOSIM and Discriminant Analysis showed significant (p < 0.05) differences at both family and genus levels in both microbiota composition and functionality as a consequence of feeding the different proteins. Differences were also significant (p < 0.05) for predicted functionality parameters as determined by PICRUSt analysis. LA induced a generally healthier microbiota composition than CAS, and higher amounts of Methanobrevibacter spp. and Methanogenic_PWY were found in the LI group. LEfSe analysis of bacterial composition and functional activities revealed a number of groups/functions able to explain the different effects found with milk and legume protein isolates. In conclusion, the mostly beneficial modulation of intestinal microbiota generally found with legume-based diets is likely to be due, at least in part, to their constituent proteins.This research was supported by Consejo Superior de Investigaciones Científicas (CSIC), and the Spanish Ministry of Science and Innovation under the projects RTC-2017-6540-1, RTI2018-100934-B-I00 and AGL2017-83772-R. The work was also partially supported by the Fondo Europeo de Desarrollo Regional (FEDER) and Fondo Social Europeo (FSE) funds from the European Union.Peer reviewedMultidisciplinary Digital Publishing InstituteConsejo Superior de Investigaciones Científicas (España)Ministerio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)European CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2024202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/341631reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI//RTC-2017-6540-1info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-100934-B-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-83772-RThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.3390/nu16010149https://doi.org/10.3390/nu16010149Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3416312026-05-22T06:33:51Z
dc.title.none.fl_str_mv Dietary Milk or Isolated Legume Proteins Modulate Intestinal Microbiota Composition in Rats
title Dietary Milk or Isolated Legume Proteins Modulate Intestinal Microbiota Composition in Rats
spellingShingle Dietary Milk or Isolated Legume Proteins Modulate Intestinal Microbiota Composition in Rats
Rubio, Luis A.
Intestinal microbiota composition
Lactalbumin
Casein
Cicer arietinum
Lupinus angustifiolius
Protein isolates
Rat
title_short Dietary Milk or Isolated Legume Proteins Modulate Intestinal Microbiota Composition in Rats
title_full Dietary Milk or Isolated Legume Proteins Modulate Intestinal Microbiota Composition in Rats
title_fullStr Dietary Milk or Isolated Legume Proteins Modulate Intestinal Microbiota Composition in Rats
title_full_unstemmed Dietary Milk or Isolated Legume Proteins Modulate Intestinal Microbiota Composition in Rats
title_sort Dietary Milk or Isolated Legume Proteins Modulate Intestinal Microbiota Composition in Rats
dc.creator.none.fl_str_mv Rubio, Luis A.
author Rubio, Luis A.
author_facet Rubio, Luis A.
author_role author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Intestinal microbiota composition
Lactalbumin
Casein
Cicer arietinum
Lupinus angustifiolius
Protein isolates
Rat
topic Intestinal microbiota composition
Lactalbumin
Casein
Cicer arietinum
Lupinus angustifiolius
Protein isolates
Rat
description Shifts toward increased proteolytic fermentation, such as, for example, in athlete and high-protein weight loss diets, may alter the relative abundance of microbial species in the gut and generate bioactive, potentially deleterious metabolic products. In the current investigation, intestinal (caecal) microbiota composition was studied in rats fed diets differing only in their constituent proteins: milk (casein (CAS), lactalbumin (LA)) or legume (Cicer arietinum, Lupinus angustifolius) protein isolates (chickpea protein isolate (CPI), lupin protein isolate (LI)). ANOSIM and Discriminant Analysis showed significant (p < 0.05) differences at both family and genus levels in both microbiota composition and functionality as a consequence of feeding the different proteins. Differences were also significant (p < 0.05) for predicted functionality parameters as determined by PICRUSt analysis. LA induced a generally healthier microbiota composition than CAS, and higher amounts of Methanobrevibacter spp. and Methanogenic_PWY were found in the LI group. LEfSe analysis of bacterial composition and functional activities revealed a number of groups/functions able to explain the different effects found with milk and legume protein isolates. In conclusion, the mostly beneficial modulation of intestinal microbiota generally found with legume-based diets is likely to be due, at least in part, to their constituent proteins.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/341631
url http://hdl.handle.net/10261/341631
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI//RTC-2017-6540-1
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-100934-B-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-83772-R
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.3390/nu16010149
https://doi.org/10.3390/nu16010149
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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