Nanoplasmonic biosensor device for the monitoring of acenocoumarol therapeutic drug in plasma
Acenocoumarol (Sintrom®) is an oral anticoagulant prescribed for the treatment of a variety of thromboembolic disorders such as atrial fibrillation and thrombosis or embolism. It inhibits fibrin production preventing clot formation. Acenocoumarol has a narrow therapeutic range, and its effects depen...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2018 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:210801 |
| Acceso en línea: | https://ddd.uab.cat/record/210801 https://dx.doi.org/urn:doi:10.1016/j.bios.2018.08.011 |
| Access Level: | acceso abierto |
| Palabra clave: | Acenocoumarol LSPR immunosensor Gold nanodisks Point-of-care device |
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Nanoplasmonic biosensor device for the monitoring of acenocoumarol therapeutic drug in plasmaPeláez Gutiérrez, Enelia Cristina|||0000-0002-9482-4566Estévez, M. Carmen|||0000-0003-3694-7186Portela Otaño, Alejandro|||0000-0003-0868-346XSalvador, J. PabloMarco, María Pilar|||0000-0002-4064-1668Lechuga, Laura M|||0000-0001-5187-5358AcenocoumarolLSPR immunosensorGold nanodisksPoint-of-care deviceAcenocoumarol (Sintrom®) is an oral anticoagulant prescribed for the treatment of a variety of thromboembolic disorders such as atrial fibrillation and thrombosis or embolism. It inhibits fibrin production preventing clot formation. Acenocoumarol has a narrow therapeutic range, and its effects depend on several factors, such as body weight, age, metabolism, diet, certain medical conditions or the intake of additional drugs, among others. A higher dose may result in the risk of bleeding, while if it is too low, the risk of blood clot can increase. Complementary tools that allow the therapeutic drug monitoring (TDM) of acenocoumarol plasmatic levels from the starting of the treatment would be of paramount importance to personalize the treatment. Point-of-care (POC) devices can offer an added value in facilitating on-site monitoring (i.e. hospitals, primary care doctor or even by the patient itself) and can aid in dosage management. With this aim, we have developed a compact and simple nanoplasmonic sensing device based on gold nanodisks for the rapid monitoring of acenocoumarol, using highly specific polyclonal antibodies produced against this drug. A specific and reproducible label free indirect competitive assay has been developed and the viability of performing the evaluation directly in plasma diluted 1:1 has been demonstrated. A limit of detection (LOD) of only 0.77 ± 0.69 nM, an IC of 48.2 ± 5.12 nM and a dynamic range between 3.38 ± 1.33 nM and 1154 ± 437 nM were achieved, which easily fit within the drug plasma levels of acenocoumarol, making this approach a highly attractive option for its decentralized monitoring in human plasma. 22018-01-0120182018-01-01Articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/210801https://dx.doi.org/urn:doi:10.1016/j.bios.2018.08.011reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 SEV-2013-0295Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-1441open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2108012026-06-06T12:50:31Z |
| dc.title.none.fl_str_mv |
Nanoplasmonic biosensor device for the monitoring of acenocoumarol therapeutic drug in plasma |
| title |
Nanoplasmonic biosensor device for the monitoring of acenocoumarol therapeutic drug in plasma |
| spellingShingle |
Nanoplasmonic biosensor device for the monitoring of acenocoumarol therapeutic drug in plasma Peláez Gutiérrez, Enelia Cristina|||0000-0002-9482-4566 Acenocoumarol LSPR immunosensor Gold nanodisks Point-of-care device |
| title_short |
Nanoplasmonic biosensor device for the monitoring of acenocoumarol therapeutic drug in plasma |
| title_full |
Nanoplasmonic biosensor device for the monitoring of acenocoumarol therapeutic drug in plasma |
| title_fullStr |
Nanoplasmonic biosensor device for the monitoring of acenocoumarol therapeutic drug in plasma |
| title_full_unstemmed |
Nanoplasmonic biosensor device for the monitoring of acenocoumarol therapeutic drug in plasma |
| title_sort |
Nanoplasmonic biosensor device for the monitoring of acenocoumarol therapeutic drug in plasma |
| dc.creator.none.fl_str_mv |
Peláez Gutiérrez, Enelia Cristina|||0000-0002-9482-4566 Estévez, M. Carmen|||0000-0003-3694-7186 Portela Otaño, Alejandro|||0000-0003-0868-346X Salvador, J. Pablo Marco, María Pilar|||0000-0002-4064-1668 Lechuga, Laura M|||0000-0001-5187-5358 |
| author |
Peláez Gutiérrez, Enelia Cristina|||0000-0002-9482-4566 |
| author_facet |
Peláez Gutiérrez, Enelia Cristina|||0000-0002-9482-4566 Estévez, M. Carmen|||0000-0003-3694-7186 Portela Otaño, Alejandro|||0000-0003-0868-346X Salvador, J. Pablo Marco, María Pilar|||0000-0002-4064-1668 Lechuga, Laura M|||0000-0001-5187-5358 |
| author_role |
author |
| author2 |
Estévez, M. Carmen|||0000-0003-3694-7186 Portela Otaño, Alejandro|||0000-0003-0868-346X Salvador, J. Pablo Marco, María Pilar|||0000-0002-4064-1668 Lechuga, Laura M|||0000-0001-5187-5358 |
| author2_role |
author author author author author |
| dc.subject.none.fl_str_mv |
Acenocoumarol LSPR immunosensor Gold nanodisks Point-of-care device |
| topic |
Acenocoumarol LSPR immunosensor Gold nanodisks Point-of-care device |
| description |
Acenocoumarol (Sintrom®) is an oral anticoagulant prescribed for the treatment of a variety of thromboembolic disorders such as atrial fibrillation and thrombosis or embolism. It inhibits fibrin production preventing clot formation. Acenocoumarol has a narrow therapeutic range, and its effects depend on several factors, such as body weight, age, metabolism, diet, certain medical conditions or the intake of additional drugs, among others. A higher dose may result in the risk of bleeding, while if it is too low, the risk of blood clot can increase. Complementary tools that allow the therapeutic drug monitoring (TDM) of acenocoumarol plasmatic levels from the starting of the treatment would be of paramount importance to personalize the treatment. Point-of-care (POC) devices can offer an added value in facilitating on-site monitoring (i.e. hospitals, primary care doctor or even by the patient itself) and can aid in dosage management. With this aim, we have developed a compact and simple nanoplasmonic sensing device based on gold nanodisks for the rapid monitoring of acenocoumarol, using highly specific polyclonal antibodies produced against this drug. A specific and reproducible label free indirect competitive assay has been developed and the viability of performing the evaluation directly in plasma diluted 1:1 has been demonstrated. A limit of detection (LOD) of only 0.77 ± 0.69 nM, an IC of 48.2 ± 5.12 nM and a dynamic range between 3.38 ± 1.33 nM and 1154 ± 437 nM were achieved, which easily fit within the drug plasma levels of acenocoumarol, making this approach a highly attractive option for its decentralized monitoring in human plasma. |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2 2018-01-01 2018 2018-01-01 |
| dc.type.none.fl_str_mv |
Article http://purl.org/coar/resource_type/c_6501 AM http://purl.org/coar/version/c_ab4af688f83e57aa |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://ddd.uab.cat/record/210801 https://dx.doi.org/urn:doi:10.1016/j.bios.2018.08.011 |
| url |
https://ddd.uab.cat/record/210801 https://dx.doi.org/urn:doi:10.1016/j.bios.2018.08.011 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 SEV-2013-0295 Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-1441 |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc-nd/4.0/ |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
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application/pdf |
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reponame:Dipòsit Digital de Documents de la UAB instname:Universitat Autònoma de Barcelona |
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Dipòsit Digital de Documents de la UAB |
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