The influence of the carrier molecule on amoxicillin recognition by specific IgE in patients with immediate hypersensitivity reactions to betalactams.

Ariza, Adriana; Mayorga, Cristobalina; Salas, María; Doña, Inmaculada; Martín-Serrano, Ángela; Pérez-Inestrosa, Ezequiel; Pérez-Sala, Dolores; Guzmán, Antonio E; Montañez, María I; Torres, María J

The influence of the carrier molecule on amoxicillin recognition by specific IgE in patients with immediate hypersensitivity reactions to betalactams.

The optimal recognition of penicillin determinants, including amoxicillin (AX), by specific IgE antibodies is widely believed to require covalent binding to a carrier molecule. The nature of the carrier and its contribution to the antigenic determinant is not well known. Here we aimed to evaluate th...

Descripción completa

Detalles Bibliográficos
Autores:	Ariza, Adriana, Mayorga, Cristobalina, Salas, María, Doña, Inmaculada, Martín-Serrano, Ángela, Pérez-Inestrosa, Ezequiel, Pérez-Sala, Dolores, Guzmán, Antonio E, Montañez, María I, Torres, María J
Tipo de recurso:	artículo
Fecha de publicación:	2016
País:	España
Institución:	Instituto de Salud Carlos III (ISCIII)
Repositorio:	Repisalud
Idioma:	inglés
OAI Identifier:	oai:repisalud.isciii.es:20.500.12105/17163
Acceso en línea:	http://hdl.handle.net/20.500.12105/17163
Access Level:	acceso abierto
Palabra clave:	Adult Aged Amoxicillin Anaphylaxis Antibody Specificity Butylamines Carrier Proteins Cross Reactions Drug Hypersensitivity Female Haptens Humans Hypersensitivity, Immediate Immunoglobulin E Male Middle Aged Penicillins Polylysine Serum Albumin, Human Urticaria Young Adult beta-Lactams

id	ES_3cfd475359a2e7fb6ff70627fb58cb20
oai_identifier_str	oai:repisalud.isciii.es:20.500.12105/17163
network_acronym_str	ES
network_name_str	España
repository_id_str
spelling	The influence of the carrier molecule on amoxicillin recognition by specific IgE in patients with immediate hypersensitivity reactions to betalactams.Ariza, AdrianaMayorga, CristobalinaSalas, MaríaDoña, InmaculadaMartín-Serrano, ÁngelaPérez-Inestrosa, EzequielPérez-Sala, DoloresGuzmán, Antonio EMontañez, María ITorres, María JAdultAgedAmoxicillinAnaphylaxisAntibody SpecificityButylaminesCarrier ProteinsCross ReactionsDrug HypersensitivityFemaleHaptensHumansHypersensitivity, ImmediateImmunoglobulin EMaleMiddle AgedPenicillinsPolylysineSerum Albumin, HumanUrticariaYoung Adultbeta-LactamsThe optimal recognition of penicillin determinants, including amoxicillin (AX), by specific IgE antibodies is widely believed to require covalent binding to a carrier molecule. The nature of the carrier and its contribution to the antigenic determinant is not well known. Here we aimed to evaluate the specific-IgE recognition of different AX-derived structures. We studied patients with immediate hypersensitivity reactions to AX, classified as selective or cross-reactors to penicillins. Competitive immunoassays were performed using AX itself, amoxicilloic acid, AX bound to butylamine (AXO-BA) or to human serum albumin (AXO-HSA) in the fluid phase, as inhibitors, and amoxicilloyl-poli-L-lysine (AXO-PLL) in the solid-phase. Two distinct patterns of AX recognition by IgE were found: Group A showed a higher recognition of AX itself and AX-modified components of low molecular weights, whilst Group B showed similar recognition of both unconjugated and conjugated AX. Amoxicilloic acid was poorly recognized in both groups, which reinforces the need for AX conjugation to a carrier for optimal recognition. Remarkably, IgE recognition in Group A (selective responders to AX) is influenced by the mode of binding and/or the nature of the carrier; whereas IgE in Group B (cross-responders to penicillins) recognizes AX independently of the nature of the carrier.20242024-01-1620162016-10-1220162016-10-12research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://hdl.handle.net/20.500.12105/17163reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/171632026-06-12T12:43:37Z
dc.title.none.fl_str_mv	The influence of the carrier molecule on amoxicillin recognition by specific IgE in patients with immediate hypersensitivity reactions to betalactams.
title	The influence of the carrier molecule on amoxicillin recognition by specific IgE in patients with immediate hypersensitivity reactions to betalactams.
spellingShingle	The influence of the carrier molecule on amoxicillin recognition by specific IgE in patients with immediate hypersensitivity reactions to betalactams. Ariza, Adriana Adult Aged Amoxicillin Anaphylaxis Antibody Specificity Butylamines Carrier Proteins Cross Reactions Drug Hypersensitivity Female Haptens Humans Hypersensitivity, Immediate Immunoglobulin E Male Middle Aged Penicillins Polylysine Serum Albumin, Human Urticaria Young Adult beta-Lactams
title_short	The influence of the carrier molecule on amoxicillin recognition by specific IgE in patients with immediate hypersensitivity reactions to betalactams.
title_full	The influence of the carrier molecule on amoxicillin recognition by specific IgE in patients with immediate hypersensitivity reactions to betalactams.
title_fullStr	The influence of the carrier molecule on amoxicillin recognition by specific IgE in patients with immediate hypersensitivity reactions to betalactams.
title_full_unstemmed	The influence of the carrier molecule on amoxicillin recognition by specific IgE in patients with immediate hypersensitivity reactions to betalactams.
title_sort	The influence of the carrier molecule on amoxicillin recognition by specific IgE in patients with immediate hypersensitivity reactions to betalactams.
dc.creator.none.fl_str_mv	Ariza, Adriana Mayorga, Cristobalina Salas, María Doña, Inmaculada Martín-Serrano, Ángela Pérez-Inestrosa, Ezequiel Pérez-Sala, Dolores Guzmán, Antonio E Montañez, María I Torres, María J
author	Ariza, Adriana
author_facet	Ariza, Adriana Mayorga, Cristobalina Salas, María Doña, Inmaculada Martín-Serrano, Ángela Pérez-Inestrosa, Ezequiel Pérez-Sala, Dolores Guzmán, Antonio E Montañez, María I Torres, María J
author_role	author
author2	Mayorga, Cristobalina Salas, María Doña, Inmaculada Martín-Serrano, Ángela Pérez-Inestrosa, Ezequiel Pérez-Sala, Dolores Guzmán, Antonio E Montañez, María I Torres, María J
author2_role	author author author author author author author author author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv	Adult Aged Amoxicillin Anaphylaxis Antibody Specificity Butylamines Carrier Proteins Cross Reactions Drug Hypersensitivity Female Haptens Humans Hypersensitivity, Immediate Immunoglobulin E Male Middle Aged Penicillins Polylysine Serum Albumin, Human Urticaria Young Adult beta-Lactams
topic	Adult Aged Amoxicillin Anaphylaxis Antibody Specificity Butylamines Carrier Proteins Cross Reactions Drug Hypersensitivity Female Haptens Humans Hypersensitivity, Immediate Immunoglobulin E Male Middle Aged Penicillins Polylysine Serum Albumin, Human Urticaria Young Adult beta-Lactams
description	The optimal recognition of penicillin determinants, including amoxicillin (AX), by specific IgE antibodies is widely believed to require covalent binding to a carrier molecule. The nature of the carrier and its contribution to the antigenic determinant is not well known. Here we aimed to evaluate the specific-IgE recognition of different AX-derived structures. We studied patients with immediate hypersensitivity reactions to AX, classified as selective or cross-reactors to penicillins. Competitive immunoassays were performed using AX itself, amoxicilloic acid, AX bound to butylamine (AXO-BA) or to human serum albumin (AXO-HSA) in the fluid phase, as inhibitors, and amoxicilloyl-poli-L-lysine (AXO-PLL) in the solid-phase. Two distinct patterns of AX recognition by IgE were found: Group A showed a higher recognition of AX itself and AX-modified components of low molecular weights, whilst Group B showed similar recognition of both unconjugated and conjugated AX. Amoxicilloic acid was poorly recognized in both groups, which reinforces the need for AX conjugation to a carrier for optimal recognition. Remarkably, IgE recognition in Group A (selective responders to AX) is influenced by the mode of binding and/or the nature of the carrier; whereas IgE in Group B (cross-responders to penicillins) recognizes AX independently of the nature of the carrier.
publishDate	2016
dc.date.none.fl_str_mv	2016 2016-10-12 2016 2016-10-12 2024 2024-01-16
dc.type.none.fl_str_mv	research article http://purl.org/coar/resource_type/c_2df8fbb1 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	http://hdl.handle.net/20.500.12105/17163
url	http://hdl.handle.net/20.500.12105/17163
dc.language.none.fl_str_mv	Inglés eng
language_invalid_str_mv	Inglés
language	eng
dc.rights.none.fl_str_mv	open access http://purl.org/coar/access_right/c_abf2 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv	info:eu-repo/semantics/openAccess
rights_invalid_str_mv	open access http://purl.org/coar/access_right/c_abf2 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv	openAccess
dc.source.none.fl_str_mv	reponame:Repisalud instname:Instituto de Salud Carlos III (ISCIII)
instname_str	Instituto de Salud Carlos III (ISCIII)
reponame_str	Repisalud
collection	Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_	1869406411445764096
score	15,812429

The influence of the carrier molecule on amoxicillin recognition by specific IgE in patients with immediate hypersensitivity reactions to betalactams.

Similares en LA Referencia