Sensitivity analysis and study of the mixing uniformity of a microfluidic mixer

We consider a microfluidic mixer based on hydrodynamic focusing, which is used to initiate the folding process of individual proteins. The folding process is initiated by quickly diluting a local denaturant concentration, and we define mixing time as the time advecting proteins experience a specifie...

Descripción completa

Detalles Bibliográficos
Autores: Ivorra, Benjamín Pierre Paul, Ramos, Angel M, López Redondo, Juana, Santiago, Juan G.
Tipo de recurso: artículo
Fecha de publicación:2015
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/33811
Acceso en línea:https://hdl.handle.net/20.500.14352/33811
Access Level:acceso abierto
Palabra clave:532.5:51-7
519.85
Microfluidic mixers
Device design
Numerical Modeling
Sensitivity analysis
Mixing uniformity
Física-Modelos matemáticos
Investigación operativa (Matemáticas)
1207 Investigación Operativa
id ES_3cdbf3bc5a831a64dbec6ae68f63ae6d
oai_identifier_str oai:docta.ucm.es:20.500.14352/33811
network_acronym_str ES
network_name_str España
repository_id_str
spelling Sensitivity analysis and study of the mixing uniformity of a microfluidic mixerIvorra, Benjamín Pierre PaulRamos, Angel MLópez Redondo, JuanaSantiago, Juan G.532.5:51-7519.85Microfluidic mixersDevice designNumerical ModelingSensitivity analysisMixing uniformityFísica-Modelos matemáticosInvestigación operativa (Matemáticas)1207 Investigación OperativaWe consider a microfluidic mixer based on hydrodynamic focusing, which is used to initiate the folding process of individual proteins. The folding process is initiated by quickly diluting a local denaturant concentration, and we define mixing time as the time advecting proteins experience a specified to achieve a local drop in denaturant concentration. In previous work, we presented a minimization of mixing time which considered optimal geometry and flow conditions, and achieved a design with a predicted mixing time of 0.10 μs. The aim of the current paper is twofold. First, we explore the sensitivity of mixing time to key geometric and flow parameters. In particular, we study the angle between inlets, the shape of the channel intersections, channel widths, mixer depth, mixer symmetry, inlet velocities, working fluid physical properties, and denaturant concentration thresholds. Second, we analyze the uniformity of mixing times as a function of inlet flow streamlines. We find the shape of the intersection, channel width, inlet velocity ratio, and asymmetries have strong effects on mixing time; while inlet angles, mixer depth, fluid properties, and concentration thresholds have weaker effects. Also, the uniformity of the mixing time is preserved for most of the inlet flow and distances of down to within about 0.4 μm of the mixer wall. We offer these analyses of sensitivities to imperfections in mixer geometry and flow conditions as a guide to experimental efforts which aim to fabricate and use these types of mixers. Our study also highlights key issues and provides a guide to the optimization and practical design of other microfluidic devices dependent on both geometry and flow conditionsCornell University LibraryUniversidad Complutense de Madrid20152015-01-0120152015-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/33811reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/338112026-06-02T12:44:21Z
dc.title.none.fl_str_mv Sensitivity analysis and study of the mixing uniformity of a microfluidic mixer
title Sensitivity analysis and study of the mixing uniformity of a microfluidic mixer
spellingShingle Sensitivity analysis and study of the mixing uniformity of a microfluidic mixer
Ivorra, Benjamín Pierre Paul
532.5:51-7
519.85
Microfluidic mixers
Device design
Numerical Modeling
Sensitivity analysis
Mixing uniformity
Física-Modelos matemáticos
Investigación operativa (Matemáticas)
1207 Investigación Operativa
title_short Sensitivity analysis and study of the mixing uniformity of a microfluidic mixer
title_full Sensitivity analysis and study of the mixing uniformity of a microfluidic mixer
title_fullStr Sensitivity analysis and study of the mixing uniformity of a microfluidic mixer
title_full_unstemmed Sensitivity analysis and study of the mixing uniformity of a microfluidic mixer
title_sort Sensitivity analysis and study of the mixing uniformity of a microfluidic mixer
dc.creator.none.fl_str_mv Ivorra, Benjamín Pierre Paul
Ramos, Angel M
López Redondo, Juana
Santiago, Juan G.
author Ivorra, Benjamín Pierre Paul
author_facet Ivorra, Benjamín Pierre Paul
Ramos, Angel M
López Redondo, Juana
Santiago, Juan G.
author_role author
author2 Ramos, Angel M
López Redondo, Juana
Santiago, Juan G.
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 532.5:51-7
519.85
Microfluidic mixers
Device design
Numerical Modeling
Sensitivity analysis
Mixing uniformity
Física-Modelos matemáticos
Investigación operativa (Matemáticas)
1207 Investigación Operativa
topic 532.5:51-7
519.85
Microfluidic mixers
Device design
Numerical Modeling
Sensitivity analysis
Mixing uniformity
Física-Modelos matemáticos
Investigación operativa (Matemáticas)
1207 Investigación Operativa
description We consider a microfluidic mixer based on hydrodynamic focusing, which is used to initiate the folding process of individual proteins. The folding process is initiated by quickly diluting a local denaturant concentration, and we define mixing time as the time advecting proteins experience a specified to achieve a local drop in denaturant concentration. In previous work, we presented a minimization of mixing time which considered optimal geometry and flow conditions, and achieved a design with a predicted mixing time of 0.10 μs. The aim of the current paper is twofold. First, we explore the sensitivity of mixing time to key geometric and flow parameters. In particular, we study the angle between inlets, the shape of the channel intersections, channel widths, mixer depth, mixer symmetry, inlet velocities, working fluid physical properties, and denaturant concentration thresholds. Second, we analyze the uniformity of mixing times as a function of inlet flow streamlines. We find the shape of the intersection, channel width, inlet velocity ratio, and asymmetries have strong effects on mixing time; while inlet angles, mixer depth, fluid properties, and concentration thresholds have weaker effects. Also, the uniformity of the mixing time is preserved for most of the inlet flow and distances of down to within about 0.4 μm of the mixer wall. We offer these analyses of sensitivities to imperfections in mixer geometry and flow conditions as a guide to experimental efforts which aim to fabricate and use these types of mixers. Our study also highlights key issues and provides a guide to the optimization and practical design of other microfluidic devices dependent on both geometry and flow conditions
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01
2015
2015-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/33811
url https://hdl.handle.net/20.500.14352/33811
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Cornell University Library
publisher.none.fl_str_mv Cornell University Library
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869406403221782528
score 15.301603

Similares en LA Referencia