Proteomic characterization of Babesia divergens extracellular vesicles

Protozoan parasites of the genus Babesia are considered a serious threat to humans and animals worldwide. Babesia parasites are naturally transmitted by ticks and infect the erythrocytes or red blood cells (RBCs) of many vertebrates causing babesiosis. Infectious species include B. divergens, which...

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Detalles Bibliográficos
Autores: Sotillo, Javier, Gonzalez, Luis Miguel, Montero-Clemente, Estrella
Tipo de recurso: conjunto de datos
Fecha de publicación:2025
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/26896
Acceso en línea:https://hdl.handle.net/20.500.12105/26896
Access Level:acceso abierto
Palabra clave:Babesia divergens
Extracellular vesicles
Proteomic characterization
Babesia
Extracellular Vesicles
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spelling Proteomic characterization of Babesia divergens extracellular vesiclesSotillo, JavierGonzalez, Luis MiguelMontero-Clemente, EstrellaBabesia divergensExtracellular vesiclesProteomic characterizationBabesiaExtracellular VesiclesProtozoan parasites of the genus Babesia are considered a serious threat to humans and animals worldwide. Babesia parasites are naturally transmitted by ticks and infect the erythrocytes or red blood cells (RBCs) of many vertebrates causing babesiosis. Infectious species include B. divergens, which causes asymptomatic to fatal babesiosis in humans and red water fever in cattle in Europe. To better understand the biology of B. divergens and to develop diagnostic and control strategies for babesiosis, we studied the extracellular vesicles (EVs) released into the environment by B. divergens-infected red blood cells (iRBCs). B. divergens parasites were cultured in human erythrocytes in vitro in a complete medium containing low human vesicle (LHV) serum. Uninfected erythrocytes (uRBC) were also maintained in the LHV complete medium. Bd-derived EVs and uRBC-derived EVs were collected and purified from the supernatants of B. divergens and uRBC cultures, respectively. Proteins were extracted from Bd-derived EVs and uRBC-derived EVs.EMBL-European Bioinformatics Institute (EMBL-EBI)20252025-09-1820252025-07-2220252025-07-22datasethttp://purl.org/coar/resource_type/c_ddb1info:eu-repo/semantics/datasetimage/pnghttps://hdl.handle.net/20.500.12105/26896reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2CC0https://creativecommons.org/public-domain/cc0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/268962026-06-12T12:43:37Z
dc.title.none.fl_str_mv Proteomic characterization of Babesia divergens extracellular vesicles
title Proteomic characterization of Babesia divergens extracellular vesicles
spellingShingle Proteomic characterization of Babesia divergens extracellular vesicles
Sotillo, Javier
Babesia divergens
Extracellular vesicles
Proteomic characterization
Babesia
Extracellular Vesicles
title_short Proteomic characterization of Babesia divergens extracellular vesicles
title_full Proteomic characterization of Babesia divergens extracellular vesicles
title_fullStr Proteomic characterization of Babesia divergens extracellular vesicles
title_full_unstemmed Proteomic characterization of Babesia divergens extracellular vesicles
title_sort Proteomic characterization of Babesia divergens extracellular vesicles
dc.creator.none.fl_str_mv Sotillo, Javier
Gonzalez, Luis Miguel
Montero-Clemente, Estrella
author Sotillo, Javier
author_facet Sotillo, Javier
Gonzalez, Luis Miguel
Montero-Clemente, Estrella
author_role author
author2 Gonzalez, Luis Miguel
Montero-Clemente, Estrella
author2_role author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Babesia divergens
Extracellular vesicles
Proteomic characterization
Babesia
Extracellular Vesicles
topic Babesia divergens
Extracellular vesicles
Proteomic characterization
Babesia
Extracellular Vesicles
description Protozoan parasites of the genus Babesia are considered a serious threat to humans and animals worldwide. Babesia parasites are naturally transmitted by ticks and infect the erythrocytes or red blood cells (RBCs) of many vertebrates causing babesiosis. Infectious species include B. divergens, which causes asymptomatic to fatal babesiosis in humans and red water fever in cattle in Europe. To better understand the biology of B. divergens and to develop diagnostic and control strategies for babesiosis, we studied the extracellular vesicles (EVs) released into the environment by B. divergens-infected red blood cells (iRBCs). B. divergens parasites were cultured in human erythrocytes in vitro in a complete medium containing low human vesicle (LHV) serum. Uninfected erythrocytes (uRBC) were also maintained in the LHV complete medium. Bd-derived EVs and uRBC-derived EVs were collected and purified from the supernatants of B. divergens and uRBC cultures, respectively. Proteins were extracted from Bd-derived EVs and uRBC-derived EVs.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025-09-18
2025
2025-07-22
2025
2025-07-22
dc.type.none.fl_str_mv dataset
http://purl.org/coar/resource_type/c_ddb1
dc.type.openaire.fl_str_mv info:eu-repo/semantics/dataset
format dataset
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.12105/26896
url https://hdl.handle.net/20.500.12105/26896
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
CC0
https://creativecommons.org/public-domain/cc0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
CC0
https://creativecommons.org/public-domain/cc0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv image/png
dc.publisher.none.fl_str_mv EMBL-European Bioinformatics Institute (EMBL-EBI)
publisher.none.fl_str_mv EMBL-European Bioinformatics Institute (EMBL-EBI)
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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