Cytological profile of antibacterial FtsZ inhibitors and synthetic peptide MciZ

17 p.-9 fig.

Detalles Bibliográficos
Autores: Araújo-Bazán, Lidia, Ruiz-Avila, Laura B., Andreu, David, Huecas, Sonia, Andreu, José Manuel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/146327
Acceso en línea:http://hdl.handle.net/10261/146327
Access Level:acceso abierto
Palabra clave:Bacterial cell division
FtsZ ring
Small molecule inhibitors
MciZ
Cytological profiling
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spelling Cytological profile of antibacterial FtsZ inhibitors and synthetic peptide MciZAraújo-Bazán, LidiaRuiz-Avila, Laura B.Andreu, DavidHuecas, SoniaAndreu, José ManuelBacterial cell divisionFtsZ ringSmall molecule inhibitorsMciZCytological profiling17 p.-9 fig.Cell division protein FtsZ is the organizer of the cytokinetic ring in almost all bacteria and a target for the discovery of new antibacterial agents that are needed to counter widespread antibiotic resistance. Bacterial cytological profiling, using quantitative microscopy, is a powerful approach for identifying the mechanism of action of antibacterial molecules affecting different cellular pathways. We have determined the cytological profile on Bacillus subtilis cells of a selection of small molecule inhibitors targeting FtsZ on different binding sites. FtsZ inhibitors lead to long undivided cells, impair the normal assembly of FtsZ into the midcell Z-rings, induce aberrant ring distributions, punctate FtsZ foci, membrane spots and also modify nucleoid length. Quantitative analysis of cell and nucleoid length combined, or the Z-ring distribution, allows categorizing FtsZ inhibitors and to distinguish them from antibiotics with other mechanisms of action, which should be useful for identifying new antibacterial FtsZ inhibitors. Biochemical assays of FtsZ polymerization and GTPase activity combined explain the cellular effects of the FtsZ polymer stabilizing agent PC190723 and its fragments. MciZ is a 40-aminoacid endogenous inhibitor of cell division normally expressed during sporulation in B. subtilis. Using FtsZ cytological profiling we have determined that exogenous synthetic MciZ is an effective inhibitor of B. subtilis cell division, Z-ring formation and localization. This finding supports our cell-based approach to screen for FtsZ inhibitors and opens new possibilities for peptide inhibitors of bacterial cell division.This work was supported by grants BFU2014-51823-R and CM 2010/BMD-2353 to JA.Peer reviewedFrontiers MediaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201720172016info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/146327reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.3389/fmicb.2016.01558Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1463272026-05-22T06:33:51Z
dc.title.none.fl_str_mv Cytological profile of antibacterial FtsZ inhibitors and synthetic peptide MciZ
title Cytological profile of antibacterial FtsZ inhibitors and synthetic peptide MciZ
spellingShingle Cytological profile of antibacterial FtsZ inhibitors and synthetic peptide MciZ
Araújo-Bazán, Lidia
Bacterial cell division
FtsZ ring
Small molecule inhibitors
MciZ
Cytological profiling
title_short Cytological profile of antibacterial FtsZ inhibitors and synthetic peptide MciZ
title_full Cytological profile of antibacterial FtsZ inhibitors and synthetic peptide MciZ
title_fullStr Cytological profile of antibacterial FtsZ inhibitors and synthetic peptide MciZ
title_full_unstemmed Cytological profile of antibacterial FtsZ inhibitors and synthetic peptide MciZ
title_sort Cytological profile of antibacterial FtsZ inhibitors and synthetic peptide MciZ
dc.creator.none.fl_str_mv Araújo-Bazán, Lidia
Ruiz-Avila, Laura B.
Andreu, David
Huecas, Sonia
Andreu, José Manuel
author Araújo-Bazán, Lidia
author_facet Araújo-Bazán, Lidia
Ruiz-Avila, Laura B.
Andreu, David
Huecas, Sonia
Andreu, José Manuel
author_role author
author2 Ruiz-Avila, Laura B.
Andreu, David
Huecas, Sonia
Andreu, José Manuel
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Bacterial cell division
FtsZ ring
Small molecule inhibitors
MciZ
Cytological profiling
topic Bacterial cell division
FtsZ ring
Small molecule inhibitors
MciZ
Cytological profiling
description 17 p.-9 fig.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/146327
url http://hdl.handle.net/10261/146327
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.3389/fmicb.2016.01558
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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