Multiplexed Ultrasensitive Determination of Adrenocorticotropin and Cortisol Hormones at a Dual Electrochemical Immunosensor

A novel dual electrochemical immunosensor for the multiplexed determination of adrenocorticotropin (ACTH) and cortisol is reported. Aminophenylboronic acid-modified dual screen-printed carbon electrodes were prepared on which the corresponding ACTH and cortisol antibodies were immobilized. Competiti...

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Detalles Bibliográficos
Autores: Moreno Guzmán, María, González Cortés, Araceli, Yáñez-Sedeño Orive, Paloma
Tipo de recurso: artículo
Fecha de publicación:2012
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/42742
Acceso en línea:https://hdl.handle.net/20.500.14352/42742
Access Level:acceso abierto
Palabra clave:543
Adrenocorticotropin
Cortisol
Electrochemical immunosensor
Phenyl boronic acid
Química analítica (Química)
2301 Química Analítica
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repository_id_str
spelling Multiplexed Ultrasensitive Determination of Adrenocorticotropin and Cortisol Hormones at a Dual Electrochemical ImmunosensorMoreno Guzmán, MaríaGonzález Cortés, AraceliYáñez-Sedeño Orive, Paloma543AdrenocorticotropinCortisolElectrochemical immunosensorPhenyl boronic acidQuímica analítica (Química)2301 Química AnalíticaA novel dual electrochemical immunosensor for the multiplexed determination of adrenocorticotropin (ACTH) and cortisol is reported. Aminophenylboronic acid-modified dual screen-printed carbon electrodes were prepared on which the corresponding ACTH and cortisol antibodies were immobilized. Competitive immunoassays involved biotinylated ACTH and alkaline phosphatase labelled streptavidin, or alkaline phosphatase labelled cortisol. Differential pulse voltammetry upon 1-naphtyl phosphate addition was employed to monitor the affinity reactions. The ranges of linearity were 5.0×10−5−0.1 and 0.1−500 ng/mL for ACTH and cortisol. The usefulness of the dual immunosensor was demonstrated by analyzing certified human serum samples with good recoveries.Wiley-VCHUniversidad Complutense de Madrid20122012-05-0120122012-05-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/42742reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/427422026-06-02T12:44:21Z
dc.title.none.fl_str_mv Multiplexed Ultrasensitive Determination of Adrenocorticotropin and Cortisol Hormones at a Dual Electrochemical Immunosensor
title Multiplexed Ultrasensitive Determination of Adrenocorticotropin and Cortisol Hormones at a Dual Electrochemical Immunosensor
spellingShingle Multiplexed Ultrasensitive Determination of Adrenocorticotropin and Cortisol Hormones at a Dual Electrochemical Immunosensor
Moreno Guzmán, María
543
Adrenocorticotropin
Cortisol
Electrochemical immunosensor
Phenyl boronic acid
Química analítica (Química)
2301 Química Analítica
title_short Multiplexed Ultrasensitive Determination of Adrenocorticotropin and Cortisol Hormones at a Dual Electrochemical Immunosensor
title_full Multiplexed Ultrasensitive Determination of Adrenocorticotropin and Cortisol Hormones at a Dual Electrochemical Immunosensor
title_fullStr Multiplexed Ultrasensitive Determination of Adrenocorticotropin and Cortisol Hormones at a Dual Electrochemical Immunosensor
title_full_unstemmed Multiplexed Ultrasensitive Determination of Adrenocorticotropin and Cortisol Hormones at a Dual Electrochemical Immunosensor
title_sort Multiplexed Ultrasensitive Determination of Adrenocorticotropin and Cortisol Hormones at a Dual Electrochemical Immunosensor
dc.creator.none.fl_str_mv Moreno Guzmán, María
González Cortés, Araceli
Yáñez-Sedeño Orive, Paloma
author Moreno Guzmán, María
author_facet Moreno Guzmán, María
González Cortés, Araceli
Yáñez-Sedeño Orive, Paloma
author_role author
author2 González Cortés, Araceli
Yáñez-Sedeño Orive, Paloma
author2_role author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 543
Adrenocorticotropin
Cortisol
Electrochemical immunosensor
Phenyl boronic acid
Química analítica (Química)
2301 Química Analítica
topic 543
Adrenocorticotropin
Cortisol
Electrochemical immunosensor
Phenyl boronic acid
Química analítica (Química)
2301 Química Analítica
description A novel dual electrochemical immunosensor for the multiplexed determination of adrenocorticotropin (ACTH) and cortisol is reported. Aminophenylboronic acid-modified dual screen-printed carbon electrodes were prepared on which the corresponding ACTH and cortisol antibodies were immobilized. Competitive immunoassays involved biotinylated ACTH and alkaline phosphatase labelled streptavidin, or alkaline phosphatase labelled cortisol. Differential pulse voltammetry upon 1-naphtyl phosphate addition was employed to monitor the affinity reactions. The ranges of linearity were 5.0×10−5−0.1 and 0.1−500 ng/mL for ACTH and cortisol. The usefulness of the dual immunosensor was demonstrated by analyzing certified human serum samples with good recoveries.
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-05-01
2012
2012-05-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/42742
url https://hdl.handle.net/20.500.14352/42742
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley-VCH
publisher.none.fl_str_mv Wiley-VCH
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869406398617485312
score 15,300724

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