Gibberella fujikuroi mutants obtained with UV radiation and N-methyl-N'-nitro-N-nitrosoguanidine
N-methyi-N'-nitro-N-nitrosoguanidine (nitrosoguanidine) and to a lesser extent UV radiation are very mutagenic for Gibberella microconidia. The recommended nitrosoguanidine doses lead to much higher frequencies of mutants than are found in other microorganisms. The frequency of mutants among th...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 1985 |
| País: | España |
| Institución: | Universidad de Sevilla (US) |
| Repositorio: | idUS. Depósito de Investigación de la Universidad de Sevilla |
| OAI Identifier: | oai:idus.us.es:11441/36600 |
| Acceso en línea: | http://hdl.handle.net/11441/36600 |
| Access Level: | acceso abierto |
| Palabra clave: | Methylnitronitrosoguanidine Etiology Fungus Gibberella fujikuroi Heredity Mutagenesis Nonhuman Priority journal Ultraviolet radiation |
| Sumario: | N-methyi-N'-nitro-N-nitrosoguanidine (nitrosoguanidine) and to a lesser extent UV radiation are very mutagenic for Gibberella microconidia. The recommended nitrosoguanidine doses lead to much higher frequencies of mutants than are found in other microorganisms. The frequency of mutants among the survivors increases linearly with the nitrosoguanidine dose (molar concentration x time); the absolute number of viable mutants in a given population reaches a maximum for a dose of ca. O.7 M · s. The microconidia are uninucleate. The onset of germination brings about increased lethality of nitrosoguanidine, but it does not modify the action of UV radiation. Mycelia are more resistaót than spores to both agents. Visible Ulumination eft'ectively prevents lethality when given immediately alter UV irradiation. Auxotrophs and color mutants are very easily obtained. Pink adenine auxotrophs and several classes of color mutants are aft'ected in the biosynthesis of the carotenoid pigment, neurosporaxanthin. |
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