Sperm characteristics and in vitro fertilization ability of thawed spermatozoa from Black Manchega ram: Electroejaculation and postmortem collection

The aim of this study was to assess two models of sperm collection on the quality and fertility of thawed spermatozoa from Black Manchega rams, a threatened breed. Sperm samples were collected by electroejaculation and postmortem from each male. Samples were diluted with Biladyl and frozen. Motility...

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Detalles Bibliográficos
Autores: García-Álvarez, Olga, Maroto-Morales, Alejandro, Martínez-Pastor, Felipe, Garde, José Julián, Ramón, Manuel, Fernández-Santos, M. Rocío, Esteso, Milagros C., Pérez-Guzmán, M. D., Soler, Ana J.
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2009
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/283881
Acceso en línea:http://hdl.handle.net/10261/283881
Access Level:acceso abierto
Palabra clave:Electroejaculated spermatozoa
Epididymal spermatozoa
Germplasm banking
Heterologous in vitro fertilization
Ram
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spelling Sperm characteristics and in vitro fertilization ability of thawed spermatozoa from Black Manchega ram: Electroejaculation and postmortem collectionGarcía-Álvarez, OlgaMaroto-Morales, AlejandroMartínez-Pastor, FelipeGarde, José JuliánRamón, ManuelFernández-Santos, M. RocíoEsteso, Milagros C.Pérez-Guzmán, M. D.Soler, Ana J.Electroejaculated spermatozoaEpididymal spermatozoaGermplasm bankingHeterologous in vitro fertilizationRamThe aim of this study was to assess two models of sperm collection on the quality and fertility of thawed spermatozoa from Black Manchega rams, a threatened breed. Sperm samples were collected by electroejaculation and postmortem from each male. Samples were diluted with Biladyl and frozen. Motility (subjective and objective by means of computer-assisted semen analysis), membrane integrity, and acrosomal status (microscopy) were assessed on fresh and thawed semen; plasmalemma integrity, mitochondrial membrane potential, DNA integrity, and acrosomal status were evaluated by flow cytometry on thawed semen. Thawed spermatozoa were used in a heterologous in vitro fertilization test. After thawing, the proportion of live spermatozoa with intact membrane (YO-PRO-1−/PI−) was higher for postmortem samples (P < 0.001), although the ratio of YO-PRO-1− spermatozoa within the PI− population was higher for ejaculated samples (P = 0.007). Likewise, the proportion of live spermatozoa having high mitochondrial membrane potential (MitoTracker+) and intact acrosomes (PNA−) was higher for postmortem samples (P < 0.001 and P < 0.001, respectively). Considering only live spermatozoa, the ratio of MitoTracker+/PNA− cells was higher for electroejaculated samples (P = 0.026 and P = 0.003). Both electroejaculated and postmortem samples fertilized oocytes. Nevertheless, electroejaculated samples yielded a higher percentage of hybrid embryos (P = 0.041). In conclusion, although postmortem spermatozoa had better sperm quality after thawing, electroejaculated spermatozoa showed higher ratios for sperm quality when only the live population was considered. Electroejaculated and postmortem samples might be used for germplasm banking of this threatened breed, but the fertility of postmortem spermatozoa might be lower.This work was supported by the Education and Science Council (PBI-05-011), by the Spanish Ministry of Education and Science (RZ2006-00006-C3), and by the Agriculture Council (PREG-05-004) of Junta de Comunidades de Castilla-La Mancha (JCCM). Olga García-Álvarez and Alejandro Maroto Morales were recipients of scholarships from INIA and JCCM, respectively. Felipe Martínez-Pastor, María Rocío Fernández-Santos, and Milagros C. Esteso were supported by the Juan de la Cierva program from the Spanish Ministry of Education and Science.Peer reviewedElsevierElsevierMinisterio de Educación y Ciencia (España)Junta de Comunidades de Castilla-La ManchaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222009info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttp://hdl.handle.net/10261/283881reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1016/j.theriogenology.2009.02.002Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2838812026-05-22T06:33:51Z
dc.title.none.fl_str_mv Sperm characteristics and in vitro fertilization ability of thawed spermatozoa from Black Manchega ram: Electroejaculation and postmortem collection
title Sperm characteristics and in vitro fertilization ability of thawed spermatozoa from Black Manchega ram: Electroejaculation and postmortem collection
spellingShingle Sperm characteristics and in vitro fertilization ability of thawed spermatozoa from Black Manchega ram: Electroejaculation and postmortem collection
García-Álvarez, Olga
Electroejaculated spermatozoa
Epididymal spermatozoa
Germplasm banking
Heterologous in vitro fertilization
Ram
title_short Sperm characteristics and in vitro fertilization ability of thawed spermatozoa from Black Manchega ram: Electroejaculation and postmortem collection
title_full Sperm characteristics and in vitro fertilization ability of thawed spermatozoa from Black Manchega ram: Electroejaculation and postmortem collection
title_fullStr Sperm characteristics and in vitro fertilization ability of thawed spermatozoa from Black Manchega ram: Electroejaculation and postmortem collection
title_full_unstemmed Sperm characteristics and in vitro fertilization ability of thawed spermatozoa from Black Manchega ram: Electroejaculation and postmortem collection
title_sort Sperm characteristics and in vitro fertilization ability of thawed spermatozoa from Black Manchega ram: Electroejaculation and postmortem collection
dc.creator.none.fl_str_mv García-Álvarez, Olga
Maroto-Morales, Alejandro
Martínez-Pastor, Felipe
Garde, José Julián
Ramón, Manuel
Fernández-Santos, M. Rocío
Esteso, Milagros C.
Pérez-Guzmán, M. D.
Soler, Ana J.
author García-Álvarez, Olga
author_facet García-Álvarez, Olga
Maroto-Morales, Alejandro
Martínez-Pastor, Felipe
Garde, José Julián
Ramón, Manuel
Fernández-Santos, M. Rocío
Esteso, Milagros C.
Pérez-Guzmán, M. D.
Soler, Ana J.
author_role author
author2 Maroto-Morales, Alejandro
Martínez-Pastor, Felipe
Garde, José Julián
Ramón, Manuel
Fernández-Santos, M. Rocío
Esteso, Milagros C.
Pérez-Guzmán, M. D.
Soler, Ana J.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Educación y Ciencia (España)
Junta de Comunidades de Castilla-La Mancha
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Electroejaculated spermatozoa
Epididymal spermatozoa
Germplasm banking
Heterologous in vitro fertilization
Ram
topic Electroejaculated spermatozoa
Epididymal spermatozoa
Germplasm banking
Heterologous in vitro fertilization
Ram
description The aim of this study was to assess two models of sperm collection on the quality and fertility of thawed spermatozoa from Black Manchega rams, a threatened breed. Sperm samples were collected by electroejaculation and postmortem from each male. Samples were diluted with Biladyl and frozen. Motility (subjective and objective by means of computer-assisted semen analysis), membrane integrity, and acrosomal status (microscopy) were assessed on fresh and thawed semen; plasmalemma integrity, mitochondrial membrane potential, DNA integrity, and acrosomal status were evaluated by flow cytometry on thawed semen. Thawed spermatozoa were used in a heterologous in vitro fertilization test. After thawing, the proportion of live spermatozoa with intact membrane (YO-PRO-1−/PI−) was higher for postmortem samples (P < 0.001), although the ratio of YO-PRO-1− spermatozoa within the PI− population was higher for ejaculated samples (P = 0.007). Likewise, the proportion of live spermatozoa having high mitochondrial membrane potential (MitoTracker+) and intact acrosomes (PNA−) was higher for postmortem samples (P < 0.001 and P < 0.001, respectively). Considering only live spermatozoa, the ratio of MitoTracker+/PNA− cells was higher for electroejaculated samples (P = 0.026 and P = 0.003). Both electroejaculated and postmortem samples fertilized oocytes. Nevertheless, electroejaculated samples yielded a higher percentage of hybrid embryos (P = 0.041). In conclusion, although postmortem spermatozoa had better sperm quality after thawing, electroejaculated spermatozoa showed higher ratios for sperm quality when only the live population was considered. Electroejaculated and postmortem samples might be used for germplasm banking of this threatened breed, but the fertility of postmortem spermatozoa might be lower.
publishDate 2009
dc.date.none.fl_str_mv 2009
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/283881
url http://hdl.handle.net/10261/283881
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1016/j.theriogenology.2009.02.002
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
Elsevier
publisher.none.fl_str_mv Elsevier
Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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