Antimicrobial activity of poly(vinyl alcohol)-poly(acrylic acid) electrospun nanofibers
Electrospun nanofibers were prepared from blends of poly(acrylic acid) (PM) and poly(vinyl alcohol) (PVA). The fibers were stabilized by heat curing at 140 degrees C via anhydride and ketone formation and crosslinking esterification. The antimicrobial effect was assessed using strains of Escherichia...
| Autores: | , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2016 |
| País: | España |
| Institución: | Universidad de Alcalá (UAH) |
| Repositorio: | e_Buah Biblioteca Digital Universidad de Alcalá |
| Idioma: | inglés |
| OAI Identifier: | oai:ebuah.uah.es:10017/64559 |
| Acceso en línea: | http://hdl.handle.net/10017/64559 https://dx.doi.org/10.1016/j.colsurfb.2016.04.052 |
| Access Level: | acceso abierto |
| Palabra clave: | Electrospinning Poly(acrylic acid) Poly(vinyl alcohol) Antimicrobial membranes Química Chemistry |
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Antimicrobial activity of poly(vinyl alcohol)-poly(acrylic acid) electrospun nanofibersSantiago Morales, JavierAmariei, Georgiana|||0000-0002-5412-6325Letón García, Pedro|||0000-0002-0384-6189Rosal García, Roberto|||0000-0003-0816-8775ElectrospinningPoly(acrylic acid)Poly(vinyl alcohol)Antimicrobial membranesQuímicaChemistryElectrospun nanofibers were prepared from blends of poly(acrylic acid) (PM) and poly(vinyl alcohol) (PVA). The fibers were stabilized by heat curing at 140 degrees C via anhydride and ketone formation and crosslinking esterification. The antimicrobial effect was assessed using strains of Escherichia coli and Staphylococcus aureus by tracking their capacity to form colonies and their metabolic impairment upon contact with PAA/PVA membranes. Membranes containing >35 wt.% PM displayed significant antibacterial activity, which was particularly high for the gram-positive S. aureus. All membranes were negatively charged, with surface zeta-potential in the (-34.5)-(-45.6) mV range, but the electrostatic interaction with the negatively charged cells was not the reason for the antimicrobial effect. Neither pH reduction nor the passing of non-crosslinked polymers to the solution affected microbial growth. The antibacterial activity was attributed to the chelation of the divalent cations stabilizing the outer cell membrane. The effect on gram-positive bacteria was attributed to the destabilization of the peptidoglycan layer. The sequestration of divalent cations was demonstrated with experiments in which calcium and a chelating agent were added to the cultures in contact with membranes. The damage to bacterial cells was tracked by measuring their surface charge and the evolution of intracellular calcium during the early stages after contact with PAA/PVA membranes. (C) 2016 Elsevier B.V. All rights reserved.20162016-10-01journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10017/64559https://dx.doi.org/10.1016/j.colsurfb.2016.04.052reponame:e_Buah Biblioteca Digital Universidad de Alcaláinstname:Universidad de Alcalá (UAH)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-ShareAlike 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccessoai:ebuah.uah.es:10017/645592026-06-18T11:13:07Z |
| dc.title.none.fl_str_mv |
Antimicrobial activity of poly(vinyl alcohol)-poly(acrylic acid) electrospun nanofibers |
| title |
Antimicrobial activity of poly(vinyl alcohol)-poly(acrylic acid) electrospun nanofibers |
| spellingShingle |
Antimicrobial activity of poly(vinyl alcohol)-poly(acrylic acid) electrospun nanofibers Santiago Morales, Javier Electrospinning Poly(acrylic acid) Poly(vinyl alcohol) Antimicrobial membranes Química Chemistry |
| title_short |
Antimicrobial activity of poly(vinyl alcohol)-poly(acrylic acid) electrospun nanofibers |
| title_full |
Antimicrobial activity of poly(vinyl alcohol)-poly(acrylic acid) electrospun nanofibers |
| title_fullStr |
Antimicrobial activity of poly(vinyl alcohol)-poly(acrylic acid) electrospun nanofibers |
| title_full_unstemmed |
Antimicrobial activity of poly(vinyl alcohol)-poly(acrylic acid) electrospun nanofibers |
| title_sort |
Antimicrobial activity of poly(vinyl alcohol)-poly(acrylic acid) electrospun nanofibers |
| dc.creator.none.fl_str_mv |
Santiago Morales, Javier Amariei, Georgiana|||0000-0002-5412-6325 Letón García, Pedro|||0000-0002-0384-6189 Rosal García, Roberto|||0000-0003-0816-8775 |
| author |
Santiago Morales, Javier |
| author_facet |
Santiago Morales, Javier Amariei, Georgiana|||0000-0002-5412-6325 Letón García, Pedro|||0000-0002-0384-6189 Rosal García, Roberto|||0000-0003-0816-8775 |
| author_role |
author |
| author2 |
Amariei, Georgiana|||0000-0002-5412-6325 Letón García, Pedro|||0000-0002-0384-6189 Rosal García, Roberto|||0000-0003-0816-8775 |
| author2_role |
author author author |
| dc.subject.none.fl_str_mv |
Electrospinning Poly(acrylic acid) Poly(vinyl alcohol) Antimicrobial membranes Química Chemistry |
| topic |
Electrospinning Poly(acrylic acid) Poly(vinyl alcohol) Antimicrobial membranes Química Chemistry |
| description |
Electrospun nanofibers were prepared from blends of poly(acrylic acid) (PM) and poly(vinyl alcohol) (PVA). The fibers were stabilized by heat curing at 140 degrees C via anhydride and ketone formation and crosslinking esterification. The antimicrobial effect was assessed using strains of Escherichia coli and Staphylococcus aureus by tracking their capacity to form colonies and their metabolic impairment upon contact with PAA/PVA membranes. Membranes containing >35 wt.% PM displayed significant antibacterial activity, which was particularly high for the gram-positive S. aureus. All membranes were negatively charged, with surface zeta-potential in the (-34.5)-(-45.6) mV range, but the electrostatic interaction with the negatively charged cells was not the reason for the antimicrobial effect. Neither pH reduction nor the passing of non-crosslinked polymers to the solution affected microbial growth. The antibacterial activity was attributed to the chelation of the divalent cations stabilizing the outer cell membrane. The effect on gram-positive bacteria was attributed to the destabilization of the peptidoglycan layer. The sequestration of divalent cations was demonstrated with experiments in which calcium and a chelating agent were added to the cultures in contact with membranes. The damage to bacterial cells was tracked by measuring their surface charge and the evolution of intracellular calcium during the early stages after contact with PAA/PVA membranes. (C) 2016 Elsevier B.V. All rights reserved. |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016 2016-10-01 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 NA http://purl.org/coar/version/c_be7fb7dd8ff6fe43 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10017/64559 https://dx.doi.org/10.1016/j.colsurfb.2016.04.052 |
| url |
http://hdl.handle.net/10017/64559 https://dx.doi.org/10.1016/j.colsurfb.2016.04.052 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ |
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openAccess |
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application/pdf |
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reponame:e_Buah Biblioteca Digital Universidad de Alcalá instname:Universidad de Alcalá (UAH) |
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Universidad de Alcalá (UAH) |
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e_Buah Biblioteca Digital Universidad de Alcalá |
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e_Buah Biblioteca Digital Universidad de Alcalá |
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