Resistencia a la infección por virus de salmónidos: inducción y potenciación, in vitro e in vivo, de un estado antivírico celular
The present study aimed to analyse comparatively the protection induced by polycytidylic acid (poly I:C), a potent interferon inductor, and several salmonid fish viruses against homologous or heterologous viruses in vitro (in BF-2 cells) and in vivo in the brown trout Salmo trutta L., 1758), in sing...
| Autores: | , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2005 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/319612 |
| Acceso en línea: | http://hdl.handle.net/10261/319612 |
| Access Level: | acceso abierto |
| Palabra clave: | Medio Marino IPNV IHNV VHSV coinfecciones víricas interferón Mx inmunidad inespecífica virus de peces |
| Sumario: | The present study aimed to analyse comparatively the protection induced by polycytidylic acid (poly I:C), a potent interferon inductor, and several salmonid fish viruses against homologous or heterologous viruses in vitro (in BF-2 cells) and in vivo in the brown trout Salmo trutta L., 1758), in single or dual infections. The expression of MxmRNA protein was used as an interferon marker. The results showed strong interference activity against rhabdoviruses in the supernatants of macrophages stimulated with poly I:C and with heterologous rhabdoviruses. The antiviral effectiveness of the cytokine-like activities differed amongst the several viruses tested. Moreover, high reduction of cumulative mortality was shown in poly I:C stimulated (3 days before infection) and IPNV-IHNV coinfected trout. A correlation between stimulation, antiviral activity and mRNA-Mx protein expression was also observed. The results indicate that interferon-mediated activity plays a significant role in the loss of IHNV virulence when it is coinfected with IPNV. |
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