Variation in DNA methylation and response to short-term herbivory in Thlaspi arvense

Plant metabolic pathways and gene networks involved in the response to herbivory are well-established, but the impact of epigenetic factors as modulators of those responses is less understood. Here, we use the demethylating agent 5-azacytidine to uncover the role of DNA cytosine methylation on pheno...

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Autores: Niloya Troyee, A., Medrano, Mónica, Müller, Caroline, Alonso, Conchita
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/284943
Acceso en línea:http://hdl.handle.net/10261/284943
Access Level:acceso abierto
Palabra clave:Brassicaceae
DNA methylation
Epigenetics
Glucosinolates
Herbivory
Thlaspi arvense
id ES_3b99a86da174f790bdbc6804e78efa7d
oai_identifier_str oai:digital.csic.es:10261/284943
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Variation in DNA methylation and response to short-term herbivory in Thlaspi arvense
title Variation in DNA methylation and response to short-term herbivory in Thlaspi arvense
spellingShingle Variation in DNA methylation and response to short-term herbivory in Thlaspi arvense
Niloya Troyee, A.
Brassicaceae
DNA methylation
Epigenetics
Glucosinolates
Herbivory
Thlaspi arvense
title_short Variation in DNA methylation and response to short-term herbivory in Thlaspi arvense
title_full Variation in DNA methylation and response to short-term herbivory in Thlaspi arvense
title_fullStr Variation in DNA methylation and response to short-term herbivory in Thlaspi arvense
title_full_unstemmed Variation in DNA methylation and response to short-term herbivory in Thlaspi arvense
title_sort Variation in DNA methylation and response to short-term herbivory in Thlaspi arvense
dc.creator.none.fl_str_mv Niloya Troyee, A.
Medrano, Mónica
Müller, Caroline
Alonso, Conchita
author Niloya Troyee, A.
author_facet Niloya Troyee, A.
Medrano, Mónica
Müller, Caroline
Alonso, Conchita
author_role author
author2 Medrano, Mónica
Müller, Caroline
Alonso, Conchita
author2_role author
author
author
dc.contributor.none.fl_str_mv European Commission
Agencia Estatal de Investigación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Brassicaceae
DNA methylation
Epigenetics
Glucosinolates
Herbivory
Thlaspi arvense
topic Brassicaceae
DNA methylation
Epigenetics
Glucosinolates
Herbivory
Thlaspi arvense
description Plant metabolic pathways and gene networks involved in the response to herbivory are well-established, but the impact of epigenetic factors as modulators of those responses is less understood. Here, we use the demethylating agent 5-azacytidine to uncover the role of DNA cytosine methylation on phenotypic responses after short-term herbivory in Thlaspi arvense plants that came from two European populations with contrasting flowering phenotypes expected to differ in the response to experimental demethylation. The experimental design followed a 2 × 3 factorial design, that was replicated for each flowering-type. First, half the seeds were submerged in a water solution of 5-azacytidine and the other half only in water, as controls. Then, we assigned control and demethylated plants to three herbivory categories (i) insect herbivory, (ii) artificial herbivory, and (iii) undamaged plants. The effects of the demethylation and herbivory treatments were assessed by quantifying genome-wide global DNA cytosine methylation, concentration of leaf glucosinolates, final stem biomass, fruit and seed production, and seed size. For most of the plant traits analysed, individuals from the two flowering-types responded differently. In late-flowering plants, global DNA methylation did not differ between control and demethylated plants but it was significantly reduced by herbivory. Conversely, in early-flowering plants, demethylation at seed stage was still evident in leaf DNA of reproductive individuals whereas herbivory did not affect their global DNA methylation. In late-flowering plants, artificial herbivory imposed a stronger reduction than insect herbivory in global DNA methylation and final stem biomass, and induced higher concentration of aliphatic glucosinolates. In early-flowering plants, the effects of herbivory were non-significant for the same traits. Finally, the effect of herbivory on reproductive parameters varied with the level of demethylation and the plant flowering-type. Although further investigations with more populations and families are required to confirm our results, they suggest that the genetic background of experimental plants and timing of damage can affect the response to herbivory and point towards multifaceted genetic-epigenetic interactions in determining herbivory-induced phenotypic plasticity.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/284943
url http://hdl.handle.net/10261/284943
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104365GB-I00
Niloya Troyee, A.; Alonso, Conchita; Medrano, Mónica; Müller, Caroline; 2022; Variation in DNA methylation and response to short-term herbivory in Thlaspi arvense [Dataset]; http://doi.org/ 10.5281/zenodo.6363512
Niloya Troyee, A.; Medrano, Mónica; Müller, Caroline; Alonso, Conchita; 2022; Variation in DNA Methylation and Response to Short-Term Herbivory in Thlaspi Arvense [Preprint]; SSRN; http://dx.doi.org/10.2139/ssrn.4005961
https://doi.org/10.1016/j.flora.2022.152106
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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spelling Variation in DNA methylation and response to short-term herbivory in Thlaspi arvenseNiloya Troyee, A.Medrano, MónicaMüller, CarolineAlonso, ConchitaBrassicaceaeDNA methylationEpigeneticsGlucosinolatesHerbivoryThlaspi arvensePlant metabolic pathways and gene networks involved in the response to herbivory are well-established, but the impact of epigenetic factors as modulators of those responses is less understood. Here, we use the demethylating agent 5-azacytidine to uncover the role of DNA cytosine methylation on phenotypic responses after short-term herbivory in Thlaspi arvense plants that came from two European populations with contrasting flowering phenotypes expected to differ in the response to experimental demethylation. The experimental design followed a 2 × 3 factorial design, that was replicated for each flowering-type. First, half the seeds were submerged in a water solution of 5-azacytidine and the other half only in water, as controls. Then, we assigned control and demethylated plants to three herbivory categories (i) insect herbivory, (ii) artificial herbivory, and (iii) undamaged plants. The effects of the demethylation and herbivory treatments were assessed by quantifying genome-wide global DNA cytosine methylation, concentration of leaf glucosinolates, final stem biomass, fruit and seed production, and seed size. For most of the plant traits analysed, individuals from the two flowering-types responded differently. In late-flowering plants, global DNA methylation did not differ between control and demethylated plants but it was significantly reduced by herbivory. Conversely, in early-flowering plants, demethylation at seed stage was still evident in leaf DNA of reproductive individuals whereas herbivory did not affect their global DNA methylation. In late-flowering plants, artificial herbivory imposed a stronger reduction than insect herbivory in global DNA methylation and final stem biomass, and induced higher concentration of aliphatic glucosinolates. In early-flowering plants, the effects of herbivory were non-significant for the same traits. Finally, the effect of herbivory on reproductive parameters varied with the level of demethylation and the plant flowering-type. Although further investigations with more populations and families are required to confirm our results, they suggest that the genetic background of experimental plants and timing of damage can affect the response to herbivory and point towards multifaceted genetic-epigenetic interactions in determining herbivory-induced phenotypic plasticity.This work was supported and funded by the European Union's Horizon 2020 research and innovation program via the Marie Sklodowska-Curie ITN ‘EpiDiverse’ (grant agreement No 764965). C.A. and M.M. were also supported by the Spanish Government through the Research Project ‘Epinter’ (PID2019-104365GB-I00, Ref. AEI/10.13039/501100011033).Peer reviewedElsevierEuropean CommissionAgencia Estatal de Investigación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/284943reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104365GB-I00Niloya Troyee, A.; Alonso, Conchita; Medrano, Mónica; Müller, Caroline; 2022; Variation in DNA methylation and response to short-term herbivory in Thlaspi arvense [Dataset]; http://doi.org/ 10.5281/zenodo.6363512Niloya Troyee, A.; Medrano, Mónica; Müller, Caroline; Alonso, Conchita; 2022; Variation in DNA Methylation and Response to Short-Term Herbivory in Thlaspi Arvense [Preprint]; SSRN; http://dx.doi.org/10.2139/ssrn.4005961https://doi.org/10.1016/j.flora.2022.152106Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2849432026-05-22T06:33:51Z
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