Genomics of the expanding pine pathogen Lecanosticta acicola reveals patterns of ongoing genetic admixture
Lecanosticta acicola is the causal agent for brown spot needle blight that affects pine trees across the northern hemisphere. Based on marker genes and microsatellite data, two distinct lineages have been identified that were introduced into Europe on two separate occasions. Despite their overall di...
| Autores: | , , , , , , , , |
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| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 2024 |
| País: | España |
| Recursos: | Universitat Pompeu Fabra |
| Repositório: | Repositorio Digital de la UPF |
| OAI Identifier: | oai:repositori.upf.edu:10230/60868 |
| Acesso em linha: | http://hdl.handle.net/10230/60868 http://dx.doi.org/10.1128/msystems.00928-23 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Lecanosticta acicola Admixture Comparative genomics Needle blight Plant pathogen Population genomics |
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Genomics of the expanding pine pathogen Lecanosticta acicola reveals patterns of ongoing genetic admixtureMarcet Houben, MarinaCruz, FernandoGómez Garrido, JèssicaAlioto, TylerNúñez Rodríguez, Juan CarlosMesanza, NebaiGut, MartaIturritxa, EugeniaGabaldón Estevan, Juan Antonio, 1973-Lecanosticta acicolaAdmixtureComparative genomicsNeedle blightPlant pathogenPopulation genomicsLecanosticta acicola is the causal agent for brown spot needle blight that affects pine trees across the northern hemisphere. Based on marker genes and microsatellite data, two distinct lineages have been identified that were introduced into Europe on two separate occasions. Despite their overall distinct geographic distribution, they have been found to coexist in regions of northern Spain and France. Here, we present the first genome-wide study of Lecanosticta acicola, including assembly of the reference genome and a population genomics analysis of 70 natural isolates from northern Spain. We show that most of the isolates belong to the southern lineage but show signs of introgression with northern lineage isolates, indicating mating between the two lineages. We also identify phenotypic differences between the two lineages based on the activity profiles of 20 enzymes, with introgressed strains being more phenotypically similar to members of the southern lineage. In conclusion, we show undergoing genetic admixture between the two main lineages of L. acicola in a region of recent expansion. Importance: Lecanosticta acicola is a fungal pathogen causing severe defoliation, growth reduction, and even death in more than 70 conifer species. Despite the increasing incidence of this species, little is known about its population dynamics. Two divergent lineages have been described that have now been found together in regions of France and Spain, but it is unknown how these mixed populations evolve. Here we present the first reference genome for this important plant pathogenic fungi and use it to study the population genomics of 70 isolates from an affected forest in the north of Spain. We find signs of introgression between the two main lineages, indicating that active mating is occurring in this region which could propitiate the appearance of novel traits in this species. We also study the phenotypic differences across this population based on enzymatic activities on 20 compounds.TG group acknowledges support from the Spanish Ministry of Science and Innovation for grants PID2021-126067NB-I00, CPP2021-008552, PCI2022-135066-2, and PDC2022-133266-I00, cofounded by ERDF “A way of making Europe”; from the Catalan Research Agency (AGAUR) SGR01551; from the European Union’s Horizon 2020 research and innovation program (ERC-2016–724173); from the Gordon and Betty Moore Foundation (Grant GBMF9742); from the “La Caixa” foundation (Grant LCF/PR/HR21/00737), and the Instituto de Salud Carlos III (IMPACT Grant IMP/00019 and CIBERINFEC CB21/13/00061- ISCIII-SGEFI/ERDF).American Society for Microbiology202420242024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/60868http://dx.doi.org/10.1128/msystems.00928-23reponame:Repositorio Digital de la UPFinstname:Universitat Pompeu FabraInglésmSystems. 2024 Mar 19;9(3):e0092823info:eu-repo/grantAgreement/EC/H2020/724173info:eu-repo/grantAgreement/ES/3PE/PID2021-126067NB-I00info:eu-repo/grantAgreement/ES/3PE/CPP2021-008552info:eu-repo/grantAgreement/ES/3PE/PCI2022-135066-2info:eu-repo/grantAgreement/ES/3PE/PDC2022-133266-I00© 2024 Marcet-Houben et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/).http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repositori.upf.edu:10230/608682026-06-12T07:21:37Z |
| dc.title.none.fl_str_mv |
Genomics of the expanding pine pathogen Lecanosticta acicola reveals patterns of ongoing genetic admixture |
| title |
Genomics of the expanding pine pathogen Lecanosticta acicola reveals patterns of ongoing genetic admixture |
| spellingShingle |
Genomics of the expanding pine pathogen Lecanosticta acicola reveals patterns of ongoing genetic admixture Marcet Houben, Marina Lecanosticta acicola Admixture Comparative genomics Needle blight Plant pathogen Population genomics |
| title_short |
Genomics of the expanding pine pathogen Lecanosticta acicola reveals patterns of ongoing genetic admixture |
| title_full |
Genomics of the expanding pine pathogen Lecanosticta acicola reveals patterns of ongoing genetic admixture |
| title_fullStr |
Genomics of the expanding pine pathogen Lecanosticta acicola reveals patterns of ongoing genetic admixture |
| title_full_unstemmed |
Genomics of the expanding pine pathogen Lecanosticta acicola reveals patterns of ongoing genetic admixture |
| title_sort |
Genomics of the expanding pine pathogen Lecanosticta acicola reveals patterns of ongoing genetic admixture |
| dc.creator.none.fl_str_mv |
Marcet Houben, Marina Cruz, Fernando Gómez Garrido, Jèssica Alioto, Tyler Núñez Rodríguez, Juan Carlos Mesanza, Nebai Gut, Marta Iturritxa, Eugenia Gabaldón Estevan, Juan Antonio, 1973- |
| author |
Marcet Houben, Marina |
| author_facet |
Marcet Houben, Marina Cruz, Fernando Gómez Garrido, Jèssica Alioto, Tyler Núñez Rodríguez, Juan Carlos Mesanza, Nebai Gut, Marta Iturritxa, Eugenia Gabaldón Estevan, Juan Antonio, 1973- |
| author_role |
author |
| author2 |
Cruz, Fernando Gómez Garrido, Jèssica Alioto, Tyler Núñez Rodríguez, Juan Carlos Mesanza, Nebai Gut, Marta Iturritxa, Eugenia Gabaldón Estevan, Juan Antonio, 1973- |
| author2_role |
author author author author author author author author |
| dc.subject.none.fl_str_mv |
Lecanosticta acicola Admixture Comparative genomics Needle blight Plant pathogen Population genomics |
| topic |
Lecanosticta acicola Admixture Comparative genomics Needle blight Plant pathogen Population genomics |
| description |
Lecanosticta acicola is the causal agent for brown spot needle blight that affects pine trees across the northern hemisphere. Based on marker genes and microsatellite data, two distinct lineages have been identified that were introduced into Europe on two separate occasions. Despite their overall distinct geographic distribution, they have been found to coexist in regions of northern Spain and France. Here, we present the first genome-wide study of Lecanosticta acicola, including assembly of the reference genome and a population genomics analysis of 70 natural isolates from northern Spain. We show that most of the isolates belong to the southern lineage but show signs of introgression with northern lineage isolates, indicating mating between the two lineages. We also identify phenotypic differences between the two lineages based on the activity profiles of 20 enzymes, with introgressed strains being more phenotypically similar to members of the southern lineage. In conclusion, we show undergoing genetic admixture between the two main lineages of L. acicola in a region of recent expansion. Importance: Lecanosticta acicola is a fungal pathogen causing severe defoliation, growth reduction, and even death in more than 70 conifer species. Despite the increasing incidence of this species, little is known about its population dynamics. Two divergent lineages have been described that have now been found together in regions of France and Spain, but it is unknown how these mixed populations evolve. Here we present the first reference genome for this important plant pathogenic fungi and use it to study the population genomics of 70 isolates from an affected forest in the north of Spain. We find signs of introgression between the two main lineages, indicating that active mating is occurring in this region which could propitiate the appearance of novel traits in this species. We also study the phenotypic differences across this population based on enzymatic activities on 20 compounds. |
| publishDate |
2024 |
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2024 2024 2024 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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http://hdl.handle.net/10230/60868 http://dx.doi.org/10.1128/msystems.00928-23 |
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http://hdl.handle.net/10230/60868 http://dx.doi.org/10.1128/msystems.00928-23 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
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mSystems. 2024 Mar 19;9(3):e0092823 info:eu-repo/grantAgreement/EC/H2020/724173 info:eu-repo/grantAgreement/ES/3PE/PID2021-126067NB-I00 info:eu-repo/grantAgreement/ES/3PE/CPP2021-008552 info:eu-repo/grantAgreement/ES/3PE/PCI2022-135066-2 info:eu-repo/grantAgreement/ES/3PE/PDC2022-133266-I00 |
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http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by/4.0/ |
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application/pdf application/pdf |
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American Society for Microbiology |
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American Society for Microbiology |
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reponame:Repositorio Digital de la UPF instname:Universitat Pompeu Fabra |
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