The fungus Tremella mesenterica encodes the longest metallothionein currently known: gene, protein and metal binding characterization

Fungal Cu-thioneins, and among them, the paradigmatic Neurospora crassa metallothio- nein (MT) (26 residues), were once considered as the shortest MTs -the ubiquitous, versa- tile metal-binding proteins- among all organisms, and thus representatives of their primeval forms. Nowadays, fungal MTs of d...

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Autores: Iturbe Espinoza, Paul, Gil Moreno, Selene, Lin, Weiyu, Calatayud, Sara, Palacios Bonilla, Òscar, Capdevila Vidal, Mercè, Atrian i Ventura, Sílvia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/104427
Acceso en línea:https://hdl.handle.net/2445/104427
Access Level:acceso abierto
Palabra clave:Fongs
Fongs patògens
Expressió gènica
Fungi
Pathogenic fungi
Gene expression
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spelling The fungus Tremella mesenterica encodes the longest metallothionein currently known: gene, protein and metal binding characterizationIturbe Espinoza, PaulGil Moreno, SeleneLin, WeiyuCalatayud, SaraPalacios Bonilla, ÒscarCapdevila Vidal, MercèAtrian i Ventura, SílviaFongsFongs patògensExpressió gènicaFungiPathogenic fungiGene expressionFungal Cu-thioneins, and among them, the paradigmatic Neurospora crassa metallothio- nein (MT) (26 residues), were once considered as the shortest MTs -the ubiquitous, versa- tile metal-binding proteins- among all organisms, and thus representatives of their primeval forms. Nowadays, fungal MTs of diverse lengths and sequence features are known, follow- ing the huge heterogeneity of the Kingdom of Fungi. At the opposite end of N . crassa MT, the recently reported Cryptococcus neoformans CnMT1 and CnMT2 (122 and 186 aa) con- stitute the longest reported fungal MTs, having been identified as virulence factors of this pathogen. CnMTs are high-capacity Cu-thioneins that appear to be built by tandem amplifi- cation of a basic unit, a 7-Cys segment homologous to N . crassa MT. Here, we report the in silico , in vivo and in vitro study of a still longer fungal MT, belonging to Tremella mesenter- ica (TmMT), a saprophytic ascomycete. The TmMT gene has 10 exons, and it yields a 779-bp mature transcript that encodes a 257 residue-long protein. This MT is also built by repeated fragments, but of variable number of Cys: six units of the 7-Cys building blocks- CXCX 3 CSCPPGXCXCAXCP-, two fragments of six Cys, plus three Cys at the N-terminus. TmMT metal binding abilities have been analyzed through the spectrophotometric and spectrometric characterization of its recombinant Zn-, Cd- and Cu-complexes. Results allow it to be unambiguous classified as a Cu-thionein, also of extraordinary coordinating capacity. According to this feature, when the TmMT cDNA is expressed in MT-devoid yeast cells, it is capable of restoring a high Cu tolerance level. Since it is not obvious that T . mesenterica shares the same physiological needs for a high capacity Cu-binding protein with C . neoformans , the existence of this peculiar MT might be better explained on the basis of a possible role in Cu-handling for the Cu-enzymes responsible in lignin degradation pathways.Public Library of Science (PLoS)2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/104427Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1371/journal.pone.0148651PLoS One, 2016, vol. 11, num. 2, p. e0148651https://doi.org/10.1371/journal.pone.0148651cc-by (c) Iturbe Espinoza, Paul. et al., 2016http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1044272026-05-27T06:46:51Z
dc.title.none.fl_str_mv The fungus Tremella mesenterica encodes the longest metallothionein currently known: gene, protein and metal binding characterization
title The fungus Tremella mesenterica encodes the longest metallothionein currently known: gene, protein and metal binding characterization
spellingShingle The fungus Tremella mesenterica encodes the longest metallothionein currently known: gene, protein and metal binding characterization
Iturbe Espinoza, Paul
Fongs
Fongs patògens
Expressió gènica
Fungi
Pathogenic fungi
Gene expression
title_short The fungus Tremella mesenterica encodes the longest metallothionein currently known: gene, protein and metal binding characterization
title_full The fungus Tremella mesenterica encodes the longest metallothionein currently known: gene, protein and metal binding characterization
title_fullStr The fungus Tremella mesenterica encodes the longest metallothionein currently known: gene, protein and metal binding characterization
title_full_unstemmed The fungus Tremella mesenterica encodes the longest metallothionein currently known: gene, protein and metal binding characterization
title_sort The fungus Tremella mesenterica encodes the longest metallothionein currently known: gene, protein and metal binding characterization
dc.creator.none.fl_str_mv Iturbe Espinoza, Paul
Gil Moreno, Selene
Lin, Weiyu
Calatayud, Sara
Palacios Bonilla, Òscar
Capdevila Vidal, Mercè
Atrian i Ventura, Sílvia
author Iturbe Espinoza, Paul
author_facet Iturbe Espinoza, Paul
Gil Moreno, Selene
Lin, Weiyu
Calatayud, Sara
Palacios Bonilla, Òscar
Capdevila Vidal, Mercè
Atrian i Ventura, Sílvia
author_role author
author2 Gil Moreno, Selene
Lin, Weiyu
Calatayud, Sara
Palacios Bonilla, Òscar
Capdevila Vidal, Mercè
Atrian i Ventura, Sílvia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Fongs
Fongs patògens
Expressió gènica
Fungi
Pathogenic fungi
Gene expression
topic Fongs
Fongs patògens
Expressió gènica
Fungi
Pathogenic fungi
Gene expression
description Fungal Cu-thioneins, and among them, the paradigmatic Neurospora crassa metallothio- nein (MT) (26 residues), were once considered as the shortest MTs -the ubiquitous, versa- tile metal-binding proteins- among all organisms, and thus representatives of their primeval forms. Nowadays, fungal MTs of diverse lengths and sequence features are known, follow- ing the huge heterogeneity of the Kingdom of Fungi. At the opposite end of N . crassa MT, the recently reported Cryptococcus neoformans CnMT1 and CnMT2 (122 and 186 aa) con- stitute the longest reported fungal MTs, having been identified as virulence factors of this pathogen. CnMTs are high-capacity Cu-thioneins that appear to be built by tandem amplifi- cation of a basic unit, a 7-Cys segment homologous to N . crassa MT. Here, we report the in silico , in vivo and in vitro study of a still longer fungal MT, belonging to Tremella mesenter- ica (TmMT), a saprophytic ascomycete. The TmMT gene has 10 exons, and it yields a 779-bp mature transcript that encodes a 257 residue-long protein. This MT is also built by repeated fragments, but of variable number of Cys: six units of the 7-Cys building blocks- CXCX 3 CSCPPGXCXCAXCP-, two fragments of six Cys, plus three Cys at the N-terminus. TmMT metal binding abilities have been analyzed through the spectrophotometric and spectrometric characterization of its recombinant Zn-, Cd- and Cu-complexes. Results allow it to be unambiguous classified as a Cu-thionein, also of extraordinary coordinating capacity. According to this feature, when the TmMT cDNA is expressed in MT-devoid yeast cells, it is capable of restoring a high Cu tolerance level. Since it is not obvious that T . mesenterica shares the same physiological needs for a high capacity Cu-binding protein with C . neoformans , the existence of this peculiar MT might be better explained on the basis of a possible role in Cu-handling for the Cu-enzymes responsible in lignin degradation pathways.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/104427
url https://hdl.handle.net/2445/104427
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0148651
PLoS One, 2016, vol. 11, num. 2, p. e0148651
https://doi.org/10.1371/journal.pone.0148651
dc.rights.none.fl_str_mv cc-by (c) Iturbe Espinoza, Paul. et al., 2016
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Iturbe Espinoza, Paul. et al., 2016
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLoS)
publisher.none.fl_str_mv Public Library of Science (PLoS)
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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