Sustainable vine shoots-to-ethanol valorisation by a sequential acid/organosolv pretreatment

In this study, a fractionation and valorisation scheme for vine shoots is proposed for biofuel and lignin production. This agricultural waste was fractionated by acid/organosolv sequential pretreatment. In the first step, acid pretreatment was optimised at 150ºC and 1.2% H2SO4 to release hemicellulo...

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Detalles Bibliográficos
Autores: Cardoza, Diego, Contreras, María del Mar, Lara-Serrano, Marta, Morales-delaRosa, Silvia, Campos Martín, José Miguel, Romero, Inmaculada, Castro, Eulogio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/390059
Acceso en línea:http://hdl.handle.net/10261/390059
https://api.elsevier.com/content/abstract/scopus_id/85183929581
Access Level:acceso abierto
Palabra clave:Co-fermentation
Ethanol
Lignin
Organosolv pretreatment
Sugars
Vine shoots
Descripción
Sumario:In this study, a fractionation and valorisation scheme for vine shoots is proposed for biofuel and lignin production. This agricultural waste was fractionated by acid/organosolv sequential pretreatment. In the first step, acid pretreatment was optimised at 150ºC and 1.2% H2SO4 to release hemicellulosic sugars, of which 76% could be recovered. This sugar stream was co-fermented by E. coli with an ethanol yield higher than 98% after detoxification with resins or NH4OH. The solid obtained under optimal acid pretreatment conditions was delignified by organosolv treatment, and a delignification rate of 43% was reached at 180ºC. This substrate with 83% enzymatic digestibility was bio-converted into ethanol by simultaneous saccharification and fermentation, with a yield of 76%. Additionally, lignin was recovered from the organosolv liquor, aiming for the full valorisation of the biomass, which showed a syringyl/guaiacyl ratio of 0.92 by nuclear magnetic resonance, complying with the data provided for Fourier transform infrared spectroscopy and confirming the aromaticity of this fraction for further valorisation.