EV-Associated miRNAs from Peritoneal Lavage are a Source of Biomarkers in Endometrial Cancer

Endometrial cancer (EC) is the sixth most common cancer in women worldwide and is responsible for more than 89,000 deaths every year. Mortality is associated with presence of poor prognostic factors at diagnosis, i.e., diagnosis at an advanced stage, with a high grade and/or an aggressive histology....

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Authors: Roman Canal, Berta, Moiola, Cristian Pablo, Gatius, Sònia, Bonnin, Sarah, Ruiz Miró, Maria, González, Esperanza, González Tallada, Xavier, Llordella, Ivanna, Hernández Alvarez, María Isabel, Porcel, José M., Gil Moreno, Antonio, Falcón Pérez, Juan Manuel, Ponomarenko, Julia, Matias-Guiu, Xavier, 1958-, Colás, Eva
Format: article
Status:Published version
Publication Date:2019
Country:España
Institution:Universidad de Barcelona
Repository:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/171549
Online Access:https://hdl.handle.net/2445/171549
Access Level:Open access
Keyword:Càncer d'endometri
Marcadors bioquímics
Endometrial cancer
Biochemical markers
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spelling EV-Associated miRNAs from Peritoneal Lavage are a Source of Biomarkers in Endometrial CancerRoman Canal, BertaMoiola, Cristian PabloGatius, SòniaBonnin, SarahRuiz Miró, MariaGonzález, EsperanzaGonzález Tallada, XavierLlordella, IvannaHernández Alvarez, María Isabel Porcel, José M.Gil Moreno, AntonioFalcón Pérez, Juan ManuelPonomarenko, JuliaMatias-Guiu, Xavier, 1958-Colás, EvaCàncer d'endometriMarcadors bioquímicsEndometrial cancerBiochemical markersEndometrial cancer (EC) is the sixth most common cancer in women worldwide and is responsible for more than 89,000 deaths every year. Mortality is associated with presence of poor prognostic factors at diagnosis, i.e., diagnosis at an advanced stage, with a high grade and/or an aggressive histology. Development of novel approaches that would permit us to improve the clinical management of EC patients is an unmet need. In this study, we investigate a novel approach to identify highly sensitive and specific biomarkers of EC using extracellular vesicles (EVs) isolated from the peritoneal lavage of EC patients. EVs of peritoneal lavages of 25 EC patients were isolated and their miRNA content was compared with miRNAs of EVs isolated from the ascitic fluid of 25 control patients. Expression of the EV-associated miRNAs was measured using the Taqman OpenArray technology that allowed us to detect 371 miRNAs. The analysis showed that 114 miRNAs were significantly dysregulated in EC patients, among which eight miRNAs, miRNA-383-5p, miRNA-10b-5p, miRNA-34c-3p, miRNA-449b-5p, miRNA-34c-5p, miRNA-200b-3p, miRNA-2110, and miRNA-34b-3p, demonstrated a classification performance at area under the receiver operating characteristic curve (AUC) values above 0.9. This finding opens an avenue for the use of EV-associated miRNAs of peritoneal lavages as an untapped source of biomarkers for EC.MDPI2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/171549Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3390/cancers11060839Cancers, 2019, vol. 11, num. 6https://doi.org/10.3390/cancers11060839cc by (c) Roman Canal et al., 2019http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1715492026-05-27T06:46:51Z
dc.title.none.fl_str_mv EV-Associated miRNAs from Peritoneal Lavage are a Source of Biomarkers in Endometrial Cancer
title EV-Associated miRNAs from Peritoneal Lavage are a Source of Biomarkers in Endometrial Cancer
spellingShingle EV-Associated miRNAs from Peritoneal Lavage are a Source of Biomarkers in Endometrial Cancer
Roman Canal, Berta
Càncer d'endometri
Marcadors bioquímics
Endometrial cancer
Biochemical markers
title_short EV-Associated miRNAs from Peritoneal Lavage are a Source of Biomarkers in Endometrial Cancer
title_full EV-Associated miRNAs from Peritoneal Lavage are a Source of Biomarkers in Endometrial Cancer
title_fullStr EV-Associated miRNAs from Peritoneal Lavage are a Source of Biomarkers in Endometrial Cancer
title_full_unstemmed EV-Associated miRNAs from Peritoneal Lavage are a Source of Biomarkers in Endometrial Cancer
title_sort EV-Associated miRNAs from Peritoneal Lavage are a Source of Biomarkers in Endometrial Cancer
dc.creator.none.fl_str_mv Roman Canal, Berta
Moiola, Cristian Pablo
Gatius, Sònia
Bonnin, Sarah
Ruiz Miró, Maria
González, Esperanza
González Tallada, Xavier
Llordella, Ivanna
Hernández Alvarez, María Isabel
Porcel, José M.
Gil Moreno, Antonio
Falcón Pérez, Juan Manuel
Ponomarenko, Julia
Matias-Guiu, Xavier, 1958-
Colás, Eva
author Roman Canal, Berta
author_facet Roman Canal, Berta
Moiola, Cristian Pablo
Gatius, Sònia
Bonnin, Sarah
Ruiz Miró, Maria
González, Esperanza
González Tallada, Xavier
Llordella, Ivanna
Hernández Alvarez, María Isabel
Porcel, José M.
Gil Moreno, Antonio
Falcón Pérez, Juan Manuel
Ponomarenko, Julia
Matias-Guiu, Xavier, 1958-
Colás, Eva
author_role author
author2 Moiola, Cristian Pablo
Gatius, Sònia
Bonnin, Sarah
Ruiz Miró, Maria
González, Esperanza
González Tallada, Xavier
Llordella, Ivanna
Hernández Alvarez, María Isabel
Porcel, José M.
Gil Moreno, Antonio
Falcón Pérez, Juan Manuel
Ponomarenko, Julia
Matias-Guiu, Xavier, 1958-
Colás, Eva
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Càncer d'endometri
Marcadors bioquímics
Endometrial cancer
Biochemical markers
topic Càncer d'endometri
Marcadors bioquímics
Endometrial cancer
Biochemical markers
description Endometrial cancer (EC) is the sixth most common cancer in women worldwide and is responsible for more than 89,000 deaths every year. Mortality is associated with presence of poor prognostic factors at diagnosis, i.e., diagnosis at an advanced stage, with a high grade and/or an aggressive histology. Development of novel approaches that would permit us to improve the clinical management of EC patients is an unmet need. In this study, we investigate a novel approach to identify highly sensitive and specific biomarkers of EC using extracellular vesicles (EVs) isolated from the peritoneal lavage of EC patients. EVs of peritoneal lavages of 25 EC patients were isolated and their miRNA content was compared with miRNAs of EVs isolated from the ascitic fluid of 25 control patients. Expression of the EV-associated miRNAs was measured using the Taqman OpenArray technology that allowed us to detect 371 miRNAs. The analysis showed that 114 miRNAs were significantly dysregulated in EC patients, among which eight miRNAs, miRNA-383-5p, miRNA-10b-5p, miRNA-34c-3p, miRNA-449b-5p, miRNA-34c-5p, miRNA-200b-3p, miRNA-2110, and miRNA-34b-3p, demonstrated a classification performance at area under the receiver operating characteristic curve (AUC) values above 0.9. This finding opens an avenue for the use of EV-associated miRNAs of peritoneal lavages as an untapped source of biomarkers for EC.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/171549
url https://hdl.handle.net/2445/171549
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/cancers11060839
Cancers, 2019, vol. 11, num. 6
https://doi.org/10.3390/cancers11060839
dc.rights.none.fl_str_mv cc by (c) Roman Canal et al., 2019
http://creativecommons.org/licenses/by/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by (c) Roman Canal et al., 2019
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869406282943823872
score 15,300724

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