Arabidopsis CDF3 transcription factor increases carbon and nitrogen assimilation and yield in trans-grafted tomato plants

[EN] Grafting in tomato ( Solanum lycopersicum L.) has mainly been used to prevent damage by soil-borne pathogens and the negative effects of abiotic stresses, although productivity and fruit quality can also be enhanced using high vigor rootstocks. In the context of a low nutrients input agricultur...

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Autores: Renau-Morata, Begoña, Jiménez-Benavente, Eva, Gil Vilar, D., Romero-Hernández, Gara, Carrillo, Laura, Vicente-Carbajosa, Jesús, Medina, Joaquín, Cebolla Cornejo, Jaime|||0000-0002-2607-9920, Molina Romero, Rosa Victoria|||0000-0001-9922-1185, Nebauer, Sergio G.|||0000-0001-7978-6680
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/214712
Acceso en línea:https://riunet.upv.es/handle/10251/214712
Access Level:acceso abierto
Palabra clave:Tomato
Grafting
NUE
Yield
Long-distance transport
C/N metabolism
Low N inputs
Rootstock
FISIOLOGIA VEGETAL
GENETICA
02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible
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network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Arabidopsis CDF3 transcription factor increases carbon and nitrogen assimilation and yield in trans-grafted tomato plants
title Arabidopsis CDF3 transcription factor increases carbon and nitrogen assimilation and yield in trans-grafted tomato plants
spellingShingle Arabidopsis CDF3 transcription factor increases carbon and nitrogen assimilation and yield in trans-grafted tomato plants
Renau-Morata, Begoña
Tomato
Grafting
NUE
Yield
Long-distance transport
C/N metabolism
Low N inputs
Rootstock
FISIOLOGIA VEGETAL
GENETICA
02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible
title_short Arabidopsis CDF3 transcription factor increases carbon and nitrogen assimilation and yield in trans-grafted tomato plants
title_full Arabidopsis CDF3 transcription factor increases carbon and nitrogen assimilation and yield in trans-grafted tomato plants
title_fullStr Arabidopsis CDF3 transcription factor increases carbon and nitrogen assimilation and yield in trans-grafted tomato plants
title_full_unstemmed Arabidopsis CDF3 transcription factor increases carbon and nitrogen assimilation and yield in trans-grafted tomato plants
title_sort Arabidopsis CDF3 transcription factor increases carbon and nitrogen assimilation and yield in trans-grafted tomato plants
dc.creator.none.fl_str_mv Renau-Morata, Begoña
Jiménez-Benavente, Eva
Gil Vilar, D.
Romero-Hernández, Gara
Carrillo, Laura
Vicente-Carbajosa, Jesús
Medina, Joaquín
Cebolla Cornejo, Jaime|||0000-0002-2607-9920
Molina Romero, Rosa Victoria|||0000-0001-9922-1185
Nebauer, Sergio G.|||0000-0001-7978-6680
author Renau-Morata, Begoña
author_facet Renau-Morata, Begoña
Jiménez-Benavente, Eva
Gil Vilar, D.
Romero-Hernández, Gara
Carrillo, Laura
Vicente-Carbajosa, Jesús
Medina, Joaquín
Cebolla Cornejo, Jaime|||0000-0002-2607-9920
Molina Romero, Rosa Victoria|||0000-0001-9922-1185
Nebauer, Sergio G.|||0000-0001-7978-6680
author_role author
author2 Jiménez-Benavente, Eva
Gil Vilar, D.
Romero-Hernández, Gara
Carrillo, Laura
Vicente-Carbajosa, Jesús
Medina, Joaquín
Cebolla Cornejo, Jaime|||0000-0002-2607-9920
Molina Romero, Rosa Victoria|||0000-0001-9922-1185
Nebauer, Sergio G.|||0000-0001-7978-6680
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Producción Vegetal
Departamento de Biotecnología
Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana
Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural
Agencia Estatal de Investigación
Universitat Politècnica de València
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Tomato
Grafting
NUE
Yield
Long-distance transport
C/N metabolism
Low N inputs
Rootstock
FISIOLOGIA VEGETAL
GENETICA
02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible
topic Tomato
Grafting
NUE
Yield
Long-distance transport
C/N metabolism
Low N inputs
Rootstock
FISIOLOGIA VEGETAL
GENETICA
02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible
description [EN] Grafting in tomato ( Solanum lycopersicum L.) has mainly been used to prevent damage by soil-borne pathogens and the negative effects of abiotic stresses, although productivity and fruit quality can also be enhanced using high vigor rootstocks. In the context of a low nutrients input agriculture, the grafting of elite cultivars onto rootstocks displaying higher Nitrogen Use Efficiency (NUE) supports a direct strategy for yield maximization. In this study we assessed the use of plants overexpressing the Arabidopsis ( AtCDF3 ) or tomato ( SlCDF3 ) CDF3 genes, previously reported to increase NUE in tomato, as rootstocks to improve yield in the grafted scion under low N inputs. We found that the AtCDF3 gene induced greater production of sugars and amino acids, which allowed for greater biomass and fruit yield under both sufficient and limiting N supplies. Conversely, no positive impact was found with the SlCDF3 gene. Hormone analyses suggest that gibberellins (GA 4 ), auxin and cytokinins (tZ) might be involved in the AtCDF3 responses to N. The differential responses triggered by the two genes could be related, at least in part, to the mobility of the AtCDF3 transcript through the phloem to the shoot. Consistently, a higher expression of the target genes of the transcription factor, such as glutamine synthase 2 ( SlGS2 ) and GA oxidase 3 ( SlGA3ox ), involved in amino acid and gibberellin biosynthesis, respectively, was observed in the leaves of this graft combination. Altogether, our results provided further insights into the mode of action of CDF3 genes and their biotechnology potential for transgrafting approaches.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-05-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/214712
url https://riunet.upv.es/handle/10251/214712
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2020-114165RR-C21 ANALISIS MOLECULAR DE LAS RESPUESTAS A NITROGENO EN GENOTIPOS DE TOMATE QUE PRESENTAN DIFERENTE NUE
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023 PID2022-136541OB-I00 PRODUCCION DE TOMATE EN CONDICIONES DE BAJOS INSUMOS: IDENTIFICACION DE PERMEASAS DE AMINOACIDOS IMPLICADAS EN LA PARTICION DE NITROGENO AL FRUTO COMO DIANAS DE MEJORA
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 CEX2020-000999-S
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 RTA2015-00014-C02-00
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento - No comercial - Sin obra derivada (by-nc-nd)
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento - No comercial - Sin obra derivada (by-nc-nd)
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Arabidopsis CDF3 transcription factor increases carbon and nitrogen assimilation and yield in trans-grafted tomato plantsRenau-Morata, BegoñaJiménez-Benavente, EvaGil Vilar, D.Romero-Hernández, GaraCarrillo, LauraVicente-Carbajosa, JesúsMedina, JoaquínCebolla Cornejo, Jaime|||0000-0002-2607-9920Molina Romero, Rosa Victoria|||0000-0001-9922-1185Nebauer, Sergio G.|||0000-0001-7978-6680TomatoGraftingNUEYieldLong-distance transportC/N metabolismLow N inputsRootstockFISIOLOGIA VEGETALGENETICA02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible[EN] Grafting in tomato ( Solanum lycopersicum L.) has mainly been used to prevent damage by soil-borne pathogens and the negative effects of abiotic stresses, although productivity and fruit quality can also be enhanced using high vigor rootstocks. In the context of a low nutrients input agriculture, the grafting of elite cultivars onto rootstocks displaying higher Nitrogen Use Efficiency (NUE) supports a direct strategy for yield maximization. In this study we assessed the use of plants overexpressing the Arabidopsis ( AtCDF3 ) or tomato ( SlCDF3 ) CDF3 genes, previously reported to increase NUE in tomato, as rootstocks to improve yield in the grafted scion under low N inputs. We found that the AtCDF3 gene induced greater production of sugars and amino acids, which allowed for greater biomass and fruit yield under both sufficient and limiting N supplies. Conversely, no positive impact was found with the SlCDF3 gene. Hormone analyses suggest that gibberellins (GA 4 ), auxin and cytokinins (tZ) might be involved in the AtCDF3 responses to N. The differential responses triggered by the two genes could be related, at least in part, to the mobility of the AtCDF3 transcript through the phloem to the shoot. Consistently, a higher expression of the target genes of the transcription factor, such as glutamine synthase 2 ( SlGS2 ) and GA oxidase 3 ( SlGA3ox ), involved in amino acid and gibberellin biosynthesis, respectively, was observed in the leaves of this graft combination. Altogether, our results provided further insights into the mode of action of CDF3 genes and their biotechnology potential for transgrafting approaches.This work was supported by the Ministerio de Ciencia e Innovacion (MCIN) and the Agencia Estatal de Investigacion (grants RTA2015-00014-c02-00 to SGN and JM, PID2020-114165RR-C21 to JM, and PID2022-136541OB-I00 to SGN). We also want to acknowledge the "Severo Ochoa Program for Centers of Excellence in R&D" (CEX2020-000999-S) supported by MCIN/AEI/10.13039/501100011033.ElsevierDepartamento de Producción VegetalDepartamento de BiotecnologíaInstituto Universitario de Conservación y Mejora de la Agrodiversidad ValencianaEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalAgencia Estatal de InvestigaciónUniversitat Politècnica de ValènciaRepositorio Institucional de la Universitat Politècnica de València Riunet20242024-05-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/214712reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2020-114165RR-C21 ANALISIS MOLECULAR DE LAS RESPUESTAS A NITROGENO EN GENOTIPOS DE TOMATE QUE PRESENTAN DIFERENTE NUEAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023 PID2022-136541OB-I00 PRODUCCION DE TOMATE EN CONDICIONES DE BAJOS INSUMOS: IDENTIFICACION DE PERMEASAS DE AMINOACIDOS IMPLICADAS EN LA PARTICION DE NITROGENO AL FRUTO COMO DIANAS DE MEJORAAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 CEX2020-000999-SAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 RTA2015-00014-C02-00open accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/2147122026-06-13T07:49:27Z
score 15,812429

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