Identification and expression analysis of GRAS transcription factor genes involved in the control of arbuscular mycorrhizal development in tomato

The formation and functioning of arbuscular mycorrhizal (AM) symbiosis are complex and tightly regulated processes. Transcriptional regulation mechanisms have been reported to mediate gene expression changes closely associated with arbuscule formation, where nutrients move between the plant and fung...

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Autores: Ho-Plágaro, Tania, Molinero Rosales, Nuria, Fariña Flores, D., Villena Díaz, M., García-Garrido, J. M.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/183567
Acceso en línea:http://hdl.handle.net/10261/183567
Access Level:acceso abierto
Palabra clave:Arbuscular mycorrhiza
RNAi interference
Gibberellins
GRAS transcription factors
Tomato
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spelling Identification and expression analysis of GRAS transcription factor genes involved in the control of arbuscular mycorrhizal development in tomatoHo-Plágaro, TaniaMolinero Rosales, NuriaFariña Flores, D.Villena Díaz, M.García-Garrido, J. M.Arbuscular mycorrhizaRNAi interferenceGibberellinsGRAS transcription factorsTomatoThe formation and functioning of arbuscular mycorrhizal (AM) symbiosis are complex and tightly regulated processes. Transcriptional regulation mechanisms have been reported to mediate gene expression changes closely associated with arbuscule formation, where nutrients move between the plant and fungus. Numerous genes encoding transcription factors (TFs), with those belonging to the GRAS family being of particular importance, are induced upon mycorrhization. In this study, a screening for candidate transcription factor genes differentially regulated in AM tomato roots showed that more than 30% of known GRAS tomato genes are upregulated upon mycorrhization. Some AM-upregulated GRAS genes were identified as encoding for transcription factors which are putative orthologs of previously identified regulators of mycorrhization in other plant species. The symbiotic role played by other newly identified AM-upregulated GRAS genes remains unknown. Preliminary results on the involvement of tomato SlGRAS18, SlGRAS38, and SlGRAS43 from the SCL3, SCL32, and SCR clades, respectively, in mycorrhization are presented. All three showed high transcript levels in the late stages of mycorrhization, and the analysis of promoter activity demonstrated that SlGRAS18 and SlGRAS43 are significantly induced in cells containing arbuscules. When SlGRAS18 and SlGRAS38 genes were silenced using RNA interference in hairy root composite tomato plants, a delay in mycorrhizal infection was observed, while an increase in mycorrhizal colonization was observed in SlGRAS43 RNAi roots. The possible mode of action of these TFs during mycorrhization is discussed, with a particular emphasis on the potential involvement of the SHR/SCR/SCL3 module of GRAS TFs in the regulation of gibberellin signaling during mycorrhization, which is analogous to other plant developmental processes.This study was supported by grants from the Comisión Interministerial de Ciencia y Tecnología (CICYT) and Fondos Europeos de Desarrollo Regional (FEDER) through the Ministerio de Economía y Competitividad in Spain (AGL2014-52298-P, AGL2017-83871-P).Peer ReviewedFrontiers MediaMinisterio de Economía y Competitividad (España)European CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2019201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/183567reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-52298-Pinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/AGL2017-83871-Phttps://www.frontiersin.org/articles/10.3389/fpls.2019.00268/fullSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1835672026-05-22T06:33:51Z
dc.title.none.fl_str_mv Identification and expression analysis of GRAS transcription factor genes involved in the control of arbuscular mycorrhizal development in tomato
title Identification and expression analysis of GRAS transcription factor genes involved in the control of arbuscular mycorrhizal development in tomato
spellingShingle Identification and expression analysis of GRAS transcription factor genes involved in the control of arbuscular mycorrhizal development in tomato
Ho-Plágaro, Tania
Arbuscular mycorrhiza
RNAi interference
Gibberellins
GRAS transcription factors
Tomato
title_short Identification and expression analysis of GRAS transcription factor genes involved in the control of arbuscular mycorrhizal development in tomato
title_full Identification and expression analysis of GRAS transcription factor genes involved in the control of arbuscular mycorrhizal development in tomato
title_fullStr Identification and expression analysis of GRAS transcription factor genes involved in the control of arbuscular mycorrhizal development in tomato
title_full_unstemmed Identification and expression analysis of GRAS transcription factor genes involved in the control of arbuscular mycorrhizal development in tomato
title_sort Identification and expression analysis of GRAS transcription factor genes involved in the control of arbuscular mycorrhizal development in tomato
dc.creator.none.fl_str_mv Ho-Plágaro, Tania
Molinero Rosales, Nuria
Fariña Flores, D.
Villena Díaz, M.
García-Garrido, J. M.
author Ho-Plágaro, Tania
author_facet Ho-Plágaro, Tania
Molinero Rosales, Nuria
Fariña Flores, D.
Villena Díaz, M.
García-Garrido, J. M.
author_role author
author2 Molinero Rosales, Nuria
Fariña Flores, D.
Villena Díaz, M.
García-Garrido, J. M.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Arbuscular mycorrhiza
RNAi interference
Gibberellins
GRAS transcription factors
Tomato
topic Arbuscular mycorrhiza
RNAi interference
Gibberellins
GRAS transcription factors
Tomato
description The formation and functioning of arbuscular mycorrhizal (AM) symbiosis are complex and tightly regulated processes. Transcriptional regulation mechanisms have been reported to mediate gene expression changes closely associated with arbuscule formation, where nutrients move between the plant and fungus. Numerous genes encoding transcription factors (TFs), with those belonging to the GRAS family being of particular importance, are induced upon mycorrhization. In this study, a screening for candidate transcription factor genes differentially regulated in AM tomato roots showed that more than 30% of known GRAS tomato genes are upregulated upon mycorrhization. Some AM-upregulated GRAS genes were identified as encoding for transcription factors which are putative orthologs of previously identified regulators of mycorrhization in other plant species. The symbiotic role played by other newly identified AM-upregulated GRAS genes remains unknown. Preliminary results on the involvement of tomato SlGRAS18, SlGRAS38, and SlGRAS43 from the SCL3, SCL32, and SCR clades, respectively, in mycorrhization are presented. All three showed high transcript levels in the late stages of mycorrhization, and the analysis of promoter activity demonstrated that SlGRAS18 and SlGRAS43 are significantly induced in cells containing arbuscules. When SlGRAS18 and SlGRAS38 genes were silenced using RNA interference in hairy root composite tomato plants, a delay in mycorrhizal infection was observed, while an increase in mycorrhizal colonization was observed in SlGRAS43 RNAi roots. The possible mode of action of these TFs during mycorrhization is discussed, with a particular emphasis on the potential involvement of the SHR/SCR/SCL3 module of GRAS TFs in the regulation of gibberellin signaling during mycorrhization, which is analogous to other plant developmental processes.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/183567
url http://hdl.handle.net/10261/183567
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-52298-P
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/AGL2017-83871-P
https://www.frontiersin.org/articles/10.3389/fpls.2019.00268/full
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
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