Tools for discrimination and analysis of lake bacterioplankton subgroups measured by flow cytometry in a high-resolution depth profile.

9 páginas, 4 figuras, 1 tabla.

Detalles Bibliográficos
Autores: Andreatta, Stefan, Wallinger, M. M., Piera, Jaume, Catalán, Jordi, Psenner, Roland, Hofer, J. S., Sommaruga, Rubén
Tipo de recurso: artículo
Fecha de publicación:2004
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/44440
Acceso en línea:http://hdl.handle.net/10261/44440
Access Level:acceso abierto
Palabra clave:Image analysis
Turbulence
Bacteria
Bacterial subgroups
Flow cytometry
Lakes
Temperature microstructure
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spelling Tools for discrimination and analysis of lake bacterioplankton subgroups measured by flow cytometry in a high-resolution depth profile.Andreatta, StefanWallinger, M. M.Piera, JaumeCatalán, JordiPsenner, RolandHofer, J. S.Sommaruga, RubénImage analysisTurbulenceBacteriaBacterial subgroupsFlow cytometryLakesTemperature microstructure9 páginas, 4 figuras, 1 tabla.A 24 m deep mesotrophic lake was sampled at 25 cm depth intervals throughout the whole water column for flow cytometric analysis of bacterioplankton, with the object of (1) assessing the suitability of image analysis algorithms to objectively discriminate bacterial subgroups in natural samples; (2) testing 2 models to evaluate the effect of changes in signal intensity versus changes in the relative abundance of the individual subgroups on bulk cell properties of the bacterial community; and (3) to examine the suitability of a numerical index for quantifying small-scale spatial variability in cell abundance. Within the heterotrophic bacterial community, 5 subgroups were detected by image analysis of DAPI fluorescence versus side scatter (SSC) histograms. On average for the whole profile, 91% (range: 86 to 94%) of all measured bacteria belonged to the 5 subgroups. Along the depth profile, abundances within these subgroups showed trends which were different from that for the bacterial community as a whole. The comparison of the 2 numerical models suggested that shifts in average DAPI and SSC signals of the whole community are better explained by changes in relative abundance within individual subgroups rather than by signal shifts within individual subgroups. Spatial variability in cell abundance for most of the heterotrophic bacterioplankton subgroups was highest in the upper 4 m of the water column, corresponding to the zone of turbulent mixing, and between 9 and 12 m, at the depth of maximum picocyanobacterial abundance. Our results show that flow cytometry in combination with image analysis of DAPI–SSC histograms at the level of bacterial subgroups allow objective assessment of the structure of the bacterial community and underpin potential sources of small-scale variability in bacterioplankton distribution.This work was supported by the Austrian Science Foundation through grants P-12718-BIO and P-14387-BIO. Participation of J.C. and J.P. was made possible through the collaborative scientific program between Austria and Spain (Grant ÖAD #17/2002).Peer reviewedInter Research201220122004info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/44440reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://www.utm.csic.es/doc/idi/pubs/Andreatta_etal04.pdfinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/444402026-05-22T06:33:51Z
dc.title.none.fl_str_mv Tools for discrimination and analysis of lake bacterioplankton subgroups measured by flow cytometry in a high-resolution depth profile.
title Tools for discrimination and analysis of lake bacterioplankton subgroups measured by flow cytometry in a high-resolution depth profile.
spellingShingle Tools for discrimination and analysis of lake bacterioplankton subgroups measured by flow cytometry in a high-resolution depth profile.
Andreatta, Stefan
Image analysis
Turbulence
Bacteria
Bacterial subgroups
Flow cytometry
Lakes
Temperature microstructure
title_short Tools for discrimination and analysis of lake bacterioplankton subgroups measured by flow cytometry in a high-resolution depth profile.
title_full Tools for discrimination and analysis of lake bacterioplankton subgroups measured by flow cytometry in a high-resolution depth profile.
title_fullStr Tools for discrimination and analysis of lake bacterioplankton subgroups measured by flow cytometry in a high-resolution depth profile.
title_full_unstemmed Tools for discrimination and analysis of lake bacterioplankton subgroups measured by flow cytometry in a high-resolution depth profile.
title_sort Tools for discrimination and analysis of lake bacterioplankton subgroups measured by flow cytometry in a high-resolution depth profile.
dc.creator.none.fl_str_mv Andreatta, Stefan
Wallinger, M. M.
Piera, Jaume
Catalán, Jordi
Psenner, Roland
Hofer, J. S.
Sommaruga, Rubén
author Andreatta, Stefan
author_facet Andreatta, Stefan
Wallinger, M. M.
Piera, Jaume
Catalán, Jordi
Psenner, Roland
Hofer, J. S.
Sommaruga, Rubén
author_role author
author2 Wallinger, M. M.
Piera, Jaume
Catalán, Jordi
Psenner, Roland
Hofer, J. S.
Sommaruga, Rubén
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Image analysis
Turbulence
Bacteria
Bacterial subgroups
Flow cytometry
Lakes
Temperature microstructure
topic Image analysis
Turbulence
Bacteria
Bacterial subgroups
Flow cytometry
Lakes
Temperature microstructure
description 9 páginas, 4 figuras, 1 tabla.
publishDate 2004
dc.date.none.fl_str_mv 2004
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/44440
url http://hdl.handle.net/10261/44440
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://www.utm.csic.es/doc/idi/pubs/Andreatta_etal04.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Inter Research
publisher.none.fl_str_mv Inter Research
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869406261093597184
score 15.811543

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