Nucleoid-associated PhaF phasin drives intracellular location and segregation of poly-hydroxyalkanoate granules in Pseudomonas putida KT2442

43 p._9 fig.-1 fig. supl.

Detalles Bibliográficos
Autores: Galán, Beatriz, Eugenio, Laura I. de, Escapa, Isabel F., Maestro, Beatriz, Sanz, Jesús M., García, José Luis, Prieto, María Auxiliadora
Tipo de recurso: artículo
Estado:Versión enviada para evaluación y publicación
Fecha de publicación:2011
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/51804
Acceso en línea:http://hdl.handle.net/10261/51804
Access Level:acceso abierto
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spelling Nucleoid-associated PhaF phasin drives intracellular location and segregation of poly-hydroxyalkanoate granules in Pseudomonas putida KT2442Galán, BeatrizEugenio, Laura I. deEscapa, Isabel F.Maestro, BeatrizSanz, Jesús M.García, José LuisPrieto, María Auxiliadora43 p._9 fig.-1 fig. supl.The PhaF is a nucleoid-associated like protein of Pseudomonas putida KT2442 involved in the polyhydroxyalkanoate (PHA) metabolism. Its primary structure shows two modular domains; the N-terminal PHA granule-binding domain (phasin domain) and the C-terminal half containing AAKP-like tandem repeats characteristic of the histone H1 family. Although the PhaF binding to PHA granules and its role as transcriptional regulator have been previously demonstrated, the cell physiology meaning of these properties remains unknown. This work demonstrates that PhaF plays a crucial role in granule localization within the cell. TEM and flow cytometry studies of cells producing granules at early growth stage demonstrated that PhaF directs the PHA granules to the centre of the cells, forming a characteristic needle array. Our studies demonstrated the existence of two markedly different cell populations in the strain lacking PhaF protein, i.e. cells with and without PHA. Complementation studies definitively demonstrated a key role of PhaF in granule segregation during the cell division ensuring the equal distribution of granules between daughter cells. In vitro studies showed that PhaF binds DNA through its C-terminal domain in a non-specific manner. All these findings suggested a main role of PhaF in PHA apparatus through interactions with the segregating chromosomeThis work was supported by grants from the Comunidad Autónoma de Madrid (P-AMB-259-0505), the Comisión Interministerial de Ciencia y Tecnología (BIO2007-67304-C02, BIO2010-21049, CSD2007-00005) and by European Union Grants (GEN 2006-27750-C5-3-E and NMP2-CT-2007-026515)Peer reviewedBlackwell Publishing2011info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Preprintinfo:eu-repo/semantics/submittedVersionhttp://hdl.handle.net/10261/51804reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1111/j.1365-2958.2010.07450.xinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/518042026-05-22T06:33:51Z
dc.title.none.fl_str_mv Nucleoid-associated PhaF phasin drives intracellular location and segregation of poly-hydroxyalkanoate granules in Pseudomonas putida KT2442
title Nucleoid-associated PhaF phasin drives intracellular location and segregation of poly-hydroxyalkanoate granules in Pseudomonas putida KT2442
spellingShingle Nucleoid-associated PhaF phasin drives intracellular location and segregation of poly-hydroxyalkanoate granules in Pseudomonas putida KT2442
Galán, Beatriz
title_short Nucleoid-associated PhaF phasin drives intracellular location and segregation of poly-hydroxyalkanoate granules in Pseudomonas putida KT2442
title_full Nucleoid-associated PhaF phasin drives intracellular location and segregation of poly-hydroxyalkanoate granules in Pseudomonas putida KT2442
title_fullStr Nucleoid-associated PhaF phasin drives intracellular location and segregation of poly-hydroxyalkanoate granules in Pseudomonas putida KT2442
title_full_unstemmed Nucleoid-associated PhaF phasin drives intracellular location and segregation of poly-hydroxyalkanoate granules in Pseudomonas putida KT2442
title_sort Nucleoid-associated PhaF phasin drives intracellular location and segregation of poly-hydroxyalkanoate granules in Pseudomonas putida KT2442
dc.creator.none.fl_str_mv Galán, Beatriz
Eugenio, Laura I. de
Escapa, Isabel F.
Maestro, Beatriz
Sanz, Jesús M.
García, José Luis
Prieto, María Auxiliadora
author Galán, Beatriz
author_facet Galán, Beatriz
Eugenio, Laura I. de
Escapa, Isabel F.
Maestro, Beatriz
Sanz, Jesús M.
García, José Luis
Prieto, María Auxiliadora
author_role author
author2 Eugenio, Laura I. de
Escapa, Isabel F.
Maestro, Beatriz
Sanz, Jesús M.
García, José Luis
Prieto, María Auxiliadora
author2_role author
author
author
author
author
author
description 43 p._9 fig.-1 fig. supl.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Preprint
info:eu-repo/semantics/submittedVersion
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/51804
url http://hdl.handle.net/10261/51804
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1111/j.1365-2958.2010.07450.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Blackwell Publishing
publisher.none.fl_str_mv Blackwell Publishing
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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