The wastewater microbiome: A novel insight for COVID-19 surveillance

Wastewater-Based Epidemiology is a tool to face and mitigate COVID-19 outbreaks by evaluating conditions in a specific community. This study aimed to analyze the microbiome profiles using nanopore technology for full-length 16S rRNA sequencing in wastewater samples collected from a penitentiary (P),...

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Detalles Bibliográficos
Autores: Gallardo-Escárate, Cristian, Valenzuela-Muñoz, Valentina, Núñez-Acuña, Gustavo, Valenzuela-Miranda, Diego, Benavente-Cartes, Bárbara, Sáez-Vera, Constanza, Urrutia, Homero E., Novoa, Beatriz, Figueras Huerta, Antonio, Roberts, Steven, Assmann, Paulina, Bravo, Marta
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/222460
Acceso en línea:http://hdl.handle.net/10261/222460
Access Level:acceso abierto
Palabra clave:SARS-CoV-2
Wastewater
Nanopore sequencing
Microbiome
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spelling The wastewater microbiome: A novel insight for COVID-19 surveillanceGallardo-Escárate, CristianValenzuela-Muñoz, ValentinaNúñez-Acuña, GustavoValenzuela-Miranda, DiegoBenavente-Cartes, BárbaraSáez-Vera, ConstanzaUrrutia, Homero E.Novoa, BeatrizFigueras Huerta, AntonioRoberts, StevenAssmann, PaulinaBravo, MartaSARS-CoV-2WastewaterNanopore sequencingMicrobiomeWastewater-Based Epidemiology is a tool to face and mitigate COVID-19 outbreaks by evaluating conditions in a specific community. This study aimed to analyze the microbiome profiles using nanopore technology for full-length 16S rRNA sequencing in wastewater samples collected from a penitentiary (P), a residential care home (RCH), and a quarantine or health care facilities (HCF). During the study, the wastewater samples from the RCH and the P were negative for SARS-CoV-2 based on qPCRs, except during the fourth week when was detected. Unexpectedly, the wastewater microbiome from RCH and P prior to week four was correlated with the samples collected from the HCF, suggesting a core bacterial community is expelled from the digest tract of individuals infected with SARS-CoV-2. The microbiota of wastewater sample positives for SARS-CoV-2 was strongly associated with enteric bacteria previously reported in patients with risk factors for COVID-19. We provide novel evidence that the wastewater microbiome associated with gastrointestinal manifestations appears to precede the SARS-CoV-2 detection in sewage. This finding suggests that the wastewaters microbiome can be applied as an indicator of community-wide SARS-CoV-2 surveillance.This study was funded by “Fondo de Emergencia Sanitaria COVID-19, Intendencia Región de Ñuble, Chile” and FONDAP #15110027 granted by National Research and Development Agency (ANID), Chile. We also thank MINSAL and MinCiencia to support this research and provide the epidemiological data.Peer reviewedElsevier BVAgencia Nacional de Investigación y Desarrollo (Chile)Ministerio de Salud (Chile)Ministerio de Ciencia, Tecnología, Conocimiento e Innovación (Chile)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/222460reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1016/j.scitotenv.2020.142867Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2224602026-05-22T06:33:51Z
dc.title.none.fl_str_mv The wastewater microbiome: A novel insight for COVID-19 surveillance
title The wastewater microbiome: A novel insight for COVID-19 surveillance
spellingShingle The wastewater microbiome: A novel insight for COVID-19 surveillance
Gallardo-Escárate, Cristian
SARS-CoV-2
Wastewater
Nanopore sequencing
Microbiome
title_short The wastewater microbiome: A novel insight for COVID-19 surveillance
title_full The wastewater microbiome: A novel insight for COVID-19 surveillance
title_fullStr The wastewater microbiome: A novel insight for COVID-19 surveillance
title_full_unstemmed The wastewater microbiome: A novel insight for COVID-19 surveillance
title_sort The wastewater microbiome: A novel insight for COVID-19 surveillance
dc.creator.none.fl_str_mv Gallardo-Escárate, Cristian
Valenzuela-Muñoz, Valentina
Núñez-Acuña, Gustavo
Valenzuela-Miranda, Diego
Benavente-Cartes, Bárbara
Sáez-Vera, Constanza
Urrutia, Homero E.
Novoa, Beatriz
Figueras Huerta, Antonio
Roberts, Steven
Assmann, Paulina
Bravo, Marta
author Gallardo-Escárate, Cristian
author_facet Gallardo-Escárate, Cristian
Valenzuela-Muñoz, Valentina
Núñez-Acuña, Gustavo
Valenzuela-Miranda, Diego
Benavente-Cartes, Bárbara
Sáez-Vera, Constanza
Urrutia, Homero E.
Novoa, Beatriz
Figueras Huerta, Antonio
Roberts, Steven
Assmann, Paulina
Bravo, Marta
author_role author
author2 Valenzuela-Muñoz, Valentina
Núñez-Acuña, Gustavo
Valenzuela-Miranda, Diego
Benavente-Cartes, Bárbara
Sáez-Vera, Constanza
Urrutia, Homero E.
Novoa, Beatriz
Figueras Huerta, Antonio
Roberts, Steven
Assmann, Paulina
Bravo, Marta
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Agencia Nacional de Investigación y Desarrollo (Chile)
Ministerio de Salud (Chile)
Ministerio de Ciencia, Tecnología, Conocimiento e Innovación (Chile)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv SARS-CoV-2
Wastewater
Nanopore sequencing
Microbiome
topic SARS-CoV-2
Wastewater
Nanopore sequencing
Microbiome
description Wastewater-Based Epidemiology is a tool to face and mitigate COVID-19 outbreaks by evaluating conditions in a specific community. This study aimed to analyze the microbiome profiles using nanopore technology for full-length 16S rRNA sequencing in wastewater samples collected from a penitentiary (P), a residential care home (RCH), and a quarantine or health care facilities (HCF). During the study, the wastewater samples from the RCH and the P were negative for SARS-CoV-2 based on qPCRs, except during the fourth week when was detected. Unexpectedly, the wastewater microbiome from RCH and P prior to week four was correlated with the samples collected from the HCF, suggesting a core bacterial community is expelled from the digest tract of individuals infected with SARS-CoV-2. The microbiota of wastewater sample positives for SARS-CoV-2 was strongly associated with enteric bacteria previously reported in patients with risk factors for COVID-19. We provide novel evidence that the wastewater microbiome associated with gastrointestinal manifestations appears to precede the SARS-CoV-2 detection in sewage. This finding suggests that the wastewaters microbiome can be applied as an indicator of community-wide SARS-CoV-2 surveillance.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/222460
url http://hdl.handle.net/10261/222460
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1016/j.scitotenv.2020.142867
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier BV
publisher.none.fl_str_mv Elsevier BV
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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