Use of ATP-Binding Cassette Subfamily A Member 13 (ABCA13) for Sensitive Detection of Focal Pathological Forms of Subclinical Bovine Paratuberculosis
[EN] Bovine paratuberculosis (PTB) is a chronic enteritis caused by Mycobacterium avium subspecies paratuberculosis (Map) that causes a heavy economic impact worldwide. Map infected animals can remain asymptomatic for years while transmitting the mycobacteria to other members of the herd. Therefore,...
| Autores: | , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Universidad Rey Juan Carlos |
| Repositorio: | BULERIA. Repositorio Institucional de la Universidad de León |
| OAI Identifier: | oai:buleria.unileon.es:10612/18833 |
| Acceso en línea: | https://www.frontiersin.org/articles/10.3389/fvets.2022.816135/full https://hdl.handle.net/10612/18833 |
| Access Level: | acceso abierto |
| Palabra clave: | Sanidad animal Paratuberculosis Subclinical infection Focal lesions Diagnosis Biomarkers ATP binding cassette subfamily A member 13 3109 Ciencias Veterinarias |
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Use of ATP-Binding Cassette Subfamily A Member 13 (ABCA13) for Sensitive Detection of Focal Pathological Forms of Subclinical Bovine ParatuberculosisBlanco Vázquez, CristinaAlonso Hearn, MartaIglesias, NataliaVázquez, PatriciaJuste, Ramón A.Garrido, Joseba M.Balseiro Morales, Ana MaríaCanive, MaríaAmado, JavierQueipo, Manuel AntonioIglesias, TaniaCasais, RosaSanidad animalParatuberculosisSubclinical infectionFocal lesionsDiagnosisBiomarkersATP binding cassette subfamily A member 133109 Ciencias Veterinarias[EN] Bovine paratuberculosis (PTB) is a chronic enteritis caused by Mycobacterium avium subspecies paratuberculosis (Map) that causes a heavy economic impact worldwide. Map infected animals can remain asymptomatic for years while transmitting the mycobacteria to other members of the herd. Therefore, accurate detection of subclinically infected animals is crucial for disease control. In a previous RNA-Seq study, we identified several mRNAs that were overexpressed in whole blood of cows with different PTB-associated histological lesions compared with control animals without detected lesions. The proteins encoded by two of these mRNAs, ATP binding cassette subfamily A member 13 (ABCA13) and Matrix Metallopeptidase 8 (MMP8) were significantly overexpressed in whole blood of animals with focal histological lesions, the most frequent pathological form in the subclinical stages of the disease. In the current study, the potential of sensitive early diagnostic tools of commercial ELISAs, based on the detection of these two biomarkers, was evaluated in serum samples of 704 Holstein Friesian cows (566 infected animals and 138 control animals from PTB-free farms). For this evaluation, infected animals were classified into three groups, according to the type of histological lesions present in their gut tissues: focal (n = 447), multifocal (n = 59), and diffuse (n = 60). The ELISA based on the detection of ABCA13 was successfully validated showing good discriminatory power between animals with focal lesions and control animals (sensitivity 82.99% and specificity 80.43%). Conversely, the MMP8-based ELISA showed a poor discriminatory power between the different histological groups and non-infected controls. The ABCA13-based ELISA showed a higher diagnostic value (0.822) than the IDEXX ELISA (0.517), the fecal bacterial isolation (0.523) and the real-time PCR (0.531) for the detection of animals with focal lesions. Overall, our results indicate that this ABCA13 ELISA greatly improves the identification of subclinically infected animals with focal lesions that are undetectable using current diagnostic methodsSIThis study was part of the I+D+i project (RTI2018- 094192-R-C22) and was funded by the Spanish MCIN/AEI/10.13039/501100011033/ Ministry of Science, Innovation and the European Regional Development Funds (FEDER Una manera de hacer Europa) and by the Gobierno del Principado de Asturias, Regional funds PCTI 2021– 2023 (GRUPIN: IDI2021-000102) co-funded by FEDER. We acknowledge the National Institute for Agricultural Research (INIA) for the scholarships of CB-V (Ayuda CPD2016-0142 financiada por MCIN/AEI/ 10.13039/501100011033 y FSE El FSE invierte en tu future) and MCFrontiers mediaSanidad AnimalFacultad de Veterinaria2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://www.frontiersin.org/articles/10.3389/fvets.2022.816135/fullhttps://hdl.handle.net/10612/18833reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad Rey Juan CarlosInglésinfo:eu-repo/grantAgreement/AEI/ Programa Estatal de I+D+i Orientada a los Retos de la Sociedad / RTI2018-094192-R-C22http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/188332026-06-24T12:43:27Z |
| dc.title.none.fl_str_mv |
Use of ATP-Binding Cassette Subfamily A Member 13 (ABCA13) for Sensitive Detection of Focal Pathological Forms of Subclinical Bovine Paratuberculosis |
| title |
Use of ATP-Binding Cassette Subfamily A Member 13 (ABCA13) for Sensitive Detection of Focal Pathological Forms of Subclinical Bovine Paratuberculosis |
| spellingShingle |
Use of ATP-Binding Cassette Subfamily A Member 13 (ABCA13) for Sensitive Detection of Focal Pathological Forms of Subclinical Bovine Paratuberculosis Blanco Vázquez, Cristina Sanidad animal Paratuberculosis Subclinical infection Focal lesions Diagnosis Biomarkers ATP binding cassette subfamily A member 13 3109 Ciencias Veterinarias |
| title_short |
Use of ATP-Binding Cassette Subfamily A Member 13 (ABCA13) for Sensitive Detection of Focal Pathological Forms of Subclinical Bovine Paratuberculosis |
| title_full |
Use of ATP-Binding Cassette Subfamily A Member 13 (ABCA13) for Sensitive Detection of Focal Pathological Forms of Subclinical Bovine Paratuberculosis |
| title_fullStr |
Use of ATP-Binding Cassette Subfamily A Member 13 (ABCA13) for Sensitive Detection of Focal Pathological Forms of Subclinical Bovine Paratuberculosis |
| title_full_unstemmed |
Use of ATP-Binding Cassette Subfamily A Member 13 (ABCA13) for Sensitive Detection of Focal Pathological Forms of Subclinical Bovine Paratuberculosis |
| title_sort |
Use of ATP-Binding Cassette Subfamily A Member 13 (ABCA13) for Sensitive Detection of Focal Pathological Forms of Subclinical Bovine Paratuberculosis |
| dc.creator.none.fl_str_mv |
Blanco Vázquez, Cristina Alonso Hearn, Marta Iglesias, Natalia Vázquez, Patricia Juste, Ramón A. Garrido, Joseba M. Balseiro Morales, Ana María Canive, María Amado, Javier Queipo, Manuel Antonio Iglesias, Tania Casais, Rosa |
| author |
Blanco Vázquez, Cristina |
| author_facet |
Blanco Vázquez, Cristina Alonso Hearn, Marta Iglesias, Natalia Vázquez, Patricia Juste, Ramón A. Garrido, Joseba M. Balseiro Morales, Ana María Canive, María Amado, Javier Queipo, Manuel Antonio Iglesias, Tania Casais, Rosa |
| author_role |
author |
| author2 |
Alonso Hearn, Marta Iglesias, Natalia Vázquez, Patricia Juste, Ramón A. Garrido, Joseba M. Balseiro Morales, Ana María Canive, María Amado, Javier Queipo, Manuel Antonio Iglesias, Tania Casais, Rosa |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Sanidad Animal Facultad de Veterinaria |
| dc.subject.none.fl_str_mv |
Sanidad animal Paratuberculosis Subclinical infection Focal lesions Diagnosis Biomarkers ATP binding cassette subfamily A member 13 3109 Ciencias Veterinarias |
| topic |
Sanidad animal Paratuberculosis Subclinical infection Focal lesions Diagnosis Biomarkers ATP binding cassette subfamily A member 13 3109 Ciencias Veterinarias |
| description |
[EN] Bovine paratuberculosis (PTB) is a chronic enteritis caused by Mycobacterium avium subspecies paratuberculosis (Map) that causes a heavy economic impact worldwide. Map infected animals can remain asymptomatic for years while transmitting the mycobacteria to other members of the herd. Therefore, accurate detection of subclinically infected animals is crucial for disease control. In a previous RNA-Seq study, we identified several mRNAs that were overexpressed in whole blood of cows with different PTB-associated histological lesions compared with control animals without detected lesions. The proteins encoded by two of these mRNAs, ATP binding cassette subfamily A member 13 (ABCA13) and Matrix Metallopeptidase 8 (MMP8) were significantly overexpressed in whole blood of animals with focal histological lesions, the most frequent pathological form in the subclinical stages of the disease. In the current study, the potential of sensitive early diagnostic tools of commercial ELISAs, based on the detection of these two biomarkers, was evaluated in serum samples of 704 Holstein Friesian cows (566 infected animals and 138 control animals from PTB-free farms). For this evaluation, infected animals were classified into three groups, according to the type of histological lesions present in their gut tissues: focal (n = 447), multifocal (n = 59), and diffuse (n = 60). The ELISA based on the detection of ABCA13 was successfully validated showing good discriminatory power between animals with focal lesions and control animals (sensitivity 82.99% and specificity 80.43%). Conversely, the MMP8-based ELISA showed a poor discriminatory power between the different histological groups and non-infected controls. The ABCA13-based ELISA showed a higher diagnostic value (0.822) than the IDEXX ELISA (0.517), the fecal bacterial isolation (0.523) and the real-time PCR (0.531) for the detection of animals with focal lesions. Overall, our results indicate that this ABCA13 ELISA greatly improves the identification of subclinically infected animals with focal lesions that are undetectable using current diagnostic methods |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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https://www.frontiersin.org/articles/10.3389/fvets.2022.816135/full https://hdl.handle.net/10612/18833 |
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https://www.frontiersin.org/articles/10.3389/fvets.2022.816135/full https://hdl.handle.net/10612/18833 |
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Inglés |
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Inglés |
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info:eu-repo/grantAgreement/AEI/ Programa Estatal de I+D+i Orientada a los Retos de la Sociedad / RTI2018-094192-R-C22 |
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http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by/4.0/ |
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openAccess |
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Frontiers media |
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