Fast Blue and Diamidino Yellow as retrograde tracers in peripheral nerves: efficacy of combined nerve injection and capsule application to transected nerves in the adult rat

Capsule application of Diamidino Yellow (DY) to the cut end of the sciatic nerve immediately followed by capsule application of Fast Blue (FB) resulted in approximate to 95% double-labelled dorsal root ganglion neurones (DRGn) and motoneurones (Mn). Nerve injection of DY followed either immediately...

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Autores: Puigdellívol Sánchez, Anna, Prats Galino, Alberto, Ruano Gil, Domingo, Molander, Carl
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2000
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/56745
Acceso en línea:https://hdl.handle.net/2445/56745
Access Level:acceso abierto
Palabra clave:Nervis espinals
Neurologia
Rates (Animals de laboratori)
Spinal nerves
Neurology
Rats as laboratory animals
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spelling Fast Blue and Diamidino Yellow as retrograde tracers in peripheral nerves: efficacy of combined nerve injection and capsule application to transected nerves in the adult ratPuigdellívol Sánchez, AnnaPrats Galino, AlbertoRuano Gil, DomingoMolander, CarlNervis espinalsNeurologiaRates (Animals de laboratori)Spinal nervesNeurologyRats as laboratory animalsCapsule application of Diamidino Yellow (DY) to the cut end of the sciatic nerve immediately followed by capsule application of Fast Blue (FB) resulted in approximate to 95% double-labelled dorsal root ganglion neurones (DRGn) and motoneurones (Mn). Nerve injection of DY followed either immediately or 2 months later by capsule application of FB resulted in approximate to 90% double-labelled DRGn and Mn, indicating that DY and FB label similar populations of DRGn and Mn, and that insignificant DY fading occurred during this period. Inversing the order of application, however, i.e. nerve injection of FB followed immediately by capsule application of DY, resulted in double labelling in only approximate to 10% of the DRGn and Mn. These percentages increased to 70% of the DRGn and 60% of the Mn when the FB injection was followed 1 or 2 months after by the DY application, indicating that DY uptake is blocked by recent administration of FB. The results indicate that DY and FB might be useful for sequential labelling before and after nerve injury as a tool to investigate the accuracy of sensory and motor regeneration.Elsevier B.V.2014201420002014info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion22 p.application/pdfhttps://hdl.handle.net/2445/56745Articles publicats en revistes (Fonaments Clínics)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: http://dx.doi.org/10.1016/S0165-0270(99)00164-8Journal of Neuroscience Methods, 2000, vol. 95, num. 2, p. 103 -110http://dx.doi.org/10.1016/S0165-0270(99)00164-8(c) Elsevier B.V., 2000info:eu-repo/semantics/openAccessoai:recercat.cat:2445/567452026-05-29T05:05:01Z
dc.title.none.fl_str_mv Fast Blue and Diamidino Yellow as retrograde tracers in peripheral nerves: efficacy of combined nerve injection and capsule application to transected nerves in the adult rat
title Fast Blue and Diamidino Yellow as retrograde tracers in peripheral nerves: efficacy of combined nerve injection and capsule application to transected nerves in the adult rat
spellingShingle Fast Blue and Diamidino Yellow as retrograde tracers in peripheral nerves: efficacy of combined nerve injection and capsule application to transected nerves in the adult rat
Puigdellívol Sánchez, Anna
Nervis espinals
Neurologia
Rates (Animals de laboratori)
Spinal nerves
Neurology
Rats as laboratory animals
title_short Fast Blue and Diamidino Yellow as retrograde tracers in peripheral nerves: efficacy of combined nerve injection and capsule application to transected nerves in the adult rat
title_full Fast Blue and Diamidino Yellow as retrograde tracers in peripheral nerves: efficacy of combined nerve injection and capsule application to transected nerves in the adult rat
title_fullStr Fast Blue and Diamidino Yellow as retrograde tracers in peripheral nerves: efficacy of combined nerve injection and capsule application to transected nerves in the adult rat
title_full_unstemmed Fast Blue and Diamidino Yellow as retrograde tracers in peripheral nerves: efficacy of combined nerve injection and capsule application to transected nerves in the adult rat
title_sort Fast Blue and Diamidino Yellow as retrograde tracers in peripheral nerves: efficacy of combined nerve injection and capsule application to transected nerves in the adult rat
dc.creator.none.fl_str_mv Puigdellívol Sánchez, Anna
Prats Galino, Alberto
Ruano Gil, Domingo
Molander, Carl
author Puigdellívol Sánchez, Anna
author_facet Puigdellívol Sánchez, Anna
Prats Galino, Alberto
Ruano Gil, Domingo
Molander, Carl
author_role author
author2 Prats Galino, Alberto
Ruano Gil, Domingo
Molander, Carl
author2_role author
author
author
dc.subject.none.fl_str_mv Nervis espinals
Neurologia
Rates (Animals de laboratori)
Spinal nerves
Neurology
Rats as laboratory animals
topic Nervis espinals
Neurologia
Rates (Animals de laboratori)
Spinal nerves
Neurology
Rats as laboratory animals
description Capsule application of Diamidino Yellow (DY) to the cut end of the sciatic nerve immediately followed by capsule application of Fast Blue (FB) resulted in approximate to 95% double-labelled dorsal root ganglion neurones (DRGn) and motoneurones (Mn). Nerve injection of DY followed either immediately or 2 months later by capsule application of FB resulted in approximate to 90% double-labelled DRGn and Mn, indicating that DY and FB label similar populations of DRGn and Mn, and that insignificant DY fading occurred during this period. Inversing the order of application, however, i.e. nerve injection of FB followed immediately by capsule application of DY, resulted in double labelling in only approximate to 10% of the DRGn and Mn. These percentages increased to 70% of the DRGn and 60% of the Mn when the FB injection was followed 1 or 2 months after by the DY application, indicating that DY uptake is blocked by recent administration of FB. The results indicate that DY and FB might be useful for sequential labelling before and after nerve injury as a tool to investigate the accuracy of sensory and motor regeneration.
publishDate 2000
dc.date.none.fl_str_mv 2000
2014
2014
2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/56745
url https://hdl.handle.net/2445/56745
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: http://dx.doi.org/10.1016/S0165-0270(99)00164-8
Journal of Neuroscience Methods, 2000, vol. 95, num. 2, p. 103 -110
http://dx.doi.org/10.1016/S0165-0270(99)00164-8
dc.rights.none.fl_str_mv (c) Elsevier B.V., 2000
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Elsevier B.V., 2000
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 22 p.
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Articles publicats en revistes (Fonaments Clínics)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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