A consensus linkage map for molecular markers and Quantitative Trait Loci associated with economically important traits in melon (Cucumis melo L.)

A number of molecular marker linkage maps have been developed for melon (Cucumis melo L.) over the last two decades. However, these maps were constructed using different marker sets, thus, making comparative analysis among maps difficult. In order to solve this problem, a consensus genetic map in me...

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Autores: Díaz, Aurora|||0000-0001-7297-1699, Fergany, Mohamed, Formisano, Gelsomina, Ziarsolo, Peio, Blanca, José, Fei, Zhanjun, Staub, Jack E., Zalapa, Juan, Cuevas, Hugo E., Dace, Gayle, Oliver, Marc, Boissot, Nathalie, Dogimont, Catherine, Pitrat, Michel, Hofstede, René, Van Koert, Paul, Harel-Beja, Rotem, Tzuri, Galil, Portnoy, Vitaly, Cohen, Shahar, Schaffer, Arthur A.., Katzir, Nurit, Xu, Yong, Zhang, Haiying, Fukino, Nobuko, Matsumoto, Satoru, Garcia-Mas, Jordi|||0000-0001-7101-9049, Monforte, Antonio J.|||0000-0003-3461-3094
Tipo de recurso: artículo
Fecha de publicación:2011
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:180284
Acceso en línea:https://ddd.uab.cat/record/180284
Access Level:acceso abierto
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spelling A consensus linkage map for molecular markers and Quantitative Trait Loci associated with economically important traits in melon (Cucumis melo L.)Díaz, Aurora|||0000-0001-7297-1699Fergany, MohamedFormisano, GelsominaZiarsolo, PeioBlanca, JoséFei, ZhanjunStaub, Jack E.Zalapa, JuanCuevas, Hugo E.Dace, GayleOliver, MarcBoissot, NathalieDogimont, CatherinePitrat, MichelHofstede, RenéVan Koert, PaulHarel-Beja, RotemTzuri, GalilPortnoy, VitalyCohen, ShaharSchaffer, Arthur A..Katzir, NuritXu, YongZhang, HaiyingFukino, NobukoMatsumoto, SatoruGarcia-Mas, Jordi|||0000-0001-7101-9049Monforte, Antonio J.|||0000-0003-3461-3094A number of molecular marker linkage maps have been developed for melon (Cucumis melo L.) over the last two decades. However, these maps were constructed using different marker sets, thus, making comparative analysis among maps difficult. In order to solve this problem, a consensus genetic map in melon was constructed using primarily highly transferable anchor markers that have broad potential use for mapping, synteny, and comparative quantitative trait loci (QTL) analysis, increasing breeding effectiveness and efficiency via markerassisted selection (MAS). - Results: Under the framework of the International Cucurbit Genomics Initiative (ICuGI, http://www.icugi.org), an integrated genetic map has been constructed by merging data from eight independent mapping experiments using a genetically diverse array of parental lines. The consensus map spans 1150 cM across the 12 melon linkage groups and is composed of 1592 markers (640 SSRs, 330 SNPs, 252 AFLPs, 239 RFLPs, 89 RAPDs, 15 IMAs, 16 indels and 11 morphological traits) with a mean marker density of 0.72 cM/marker. One hundred and ninety-six of these markers (157 SSRs, 32 SNPs, 6 indels and 1 RAPD) were newly developed, mapped or provided by industry representatives as released markers, including 27 SNPs and 5 indels from genes involved in the organic acid metabolism and transport, and 58 EST-SSRs. Additionally, 85 of 822 SSR markers contributed by Syngenta Seeds were included in the integrated map. In addition, 370 QTL controlling 62 traits from 18 previously reported mapping experiments using genetically diverse parental genotypes were also integrated into the consensus map. Some QTL associated with economically important traits detected in separate studies mapped to similar genòmic positions. For example, independently identified QTL controlling fruit shape were mapped on similar genòmic positions, suggesting that such QTL are possibly responsible for the phenotypic variability observed for this trait in a broad array of melon germplasm. - Conclusions: Even though relatively unsaturated genetic maps in a diverse set of melon market types have been published, the integrated saturated map presented herein should be considered the initial reference map for melon. Most of the mapped markers contained in the reference map are polymorphic in diverse collection of germplasm, and thus are potentially transferrable to a broad array of genetic experimentation (e.g., integration of physical and genetic maps, colinearity analysis, map-based gene cloning, epistasis dissection, and marker-assisted selection). 22011-01-0120112011-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/180284reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Ciencia e Innovación https://doi.org/10.13039/501100004837 AGL2009/12698-C02-02open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:1802842026-06-06T12:50:31Z
dc.title.none.fl_str_mv A consensus linkage map for molecular markers and Quantitative Trait Loci associated with economically important traits in melon (Cucumis melo L.)
title A consensus linkage map for molecular markers and Quantitative Trait Loci associated with economically important traits in melon (Cucumis melo L.)
spellingShingle A consensus linkage map for molecular markers and Quantitative Trait Loci associated with economically important traits in melon (Cucumis melo L.)
Díaz, Aurora|||0000-0001-7297-1699
title_short A consensus linkage map for molecular markers and Quantitative Trait Loci associated with economically important traits in melon (Cucumis melo L.)
title_full A consensus linkage map for molecular markers and Quantitative Trait Loci associated with economically important traits in melon (Cucumis melo L.)
title_fullStr A consensus linkage map for molecular markers and Quantitative Trait Loci associated with economically important traits in melon (Cucumis melo L.)
title_full_unstemmed A consensus linkage map for molecular markers and Quantitative Trait Loci associated with economically important traits in melon (Cucumis melo L.)
title_sort A consensus linkage map for molecular markers and Quantitative Trait Loci associated with economically important traits in melon (Cucumis melo L.)
dc.creator.none.fl_str_mv Díaz, Aurora|||0000-0001-7297-1699
Fergany, Mohamed
Formisano, Gelsomina
Ziarsolo, Peio
Blanca, José
Fei, Zhanjun
Staub, Jack E.
Zalapa, Juan
Cuevas, Hugo E.
Dace, Gayle
Oliver, Marc
Boissot, Nathalie
Dogimont, Catherine
Pitrat, Michel
Hofstede, René
Van Koert, Paul
Harel-Beja, Rotem
Tzuri, Galil
Portnoy, Vitaly
Cohen, Shahar
Schaffer, Arthur A..
Katzir, Nurit
Xu, Yong
Zhang, Haiying
Fukino, Nobuko
Matsumoto, Satoru
Garcia-Mas, Jordi|||0000-0001-7101-9049
Monforte, Antonio J.|||0000-0003-3461-3094
author Díaz, Aurora|||0000-0001-7297-1699
author_facet Díaz, Aurora|||0000-0001-7297-1699
Fergany, Mohamed
Formisano, Gelsomina
Ziarsolo, Peio
Blanca, José
Fei, Zhanjun
Staub, Jack E.
Zalapa, Juan
Cuevas, Hugo E.
Dace, Gayle
Oliver, Marc
Boissot, Nathalie
Dogimont, Catherine
Pitrat, Michel
Hofstede, René
Van Koert, Paul
Harel-Beja, Rotem
Tzuri, Galil
Portnoy, Vitaly
Cohen, Shahar
Schaffer, Arthur A..
Katzir, Nurit
Xu, Yong
Zhang, Haiying
Fukino, Nobuko
Matsumoto, Satoru
Garcia-Mas, Jordi|||0000-0001-7101-9049
Monforte, Antonio J.|||0000-0003-3461-3094
author_role author
author2 Fergany, Mohamed
Formisano, Gelsomina
Ziarsolo, Peio
Blanca, José
Fei, Zhanjun
Staub, Jack E.
Zalapa, Juan
Cuevas, Hugo E.
Dace, Gayle
Oliver, Marc
Boissot, Nathalie
Dogimont, Catherine
Pitrat, Michel
Hofstede, René
Van Koert, Paul
Harel-Beja, Rotem
Tzuri, Galil
Portnoy, Vitaly
Cohen, Shahar
Schaffer, Arthur A..
Katzir, Nurit
Xu, Yong
Zhang, Haiying
Fukino, Nobuko
Matsumoto, Satoru
Garcia-Mas, Jordi|||0000-0001-7101-9049
Monforte, Antonio J.|||0000-0003-3461-3094
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
description A number of molecular marker linkage maps have been developed for melon (Cucumis melo L.) over the last two decades. However, these maps were constructed using different marker sets, thus, making comparative analysis among maps difficult. In order to solve this problem, a consensus genetic map in melon was constructed using primarily highly transferable anchor markers that have broad potential use for mapping, synteny, and comparative quantitative trait loci (QTL) analysis, increasing breeding effectiveness and efficiency via markerassisted selection (MAS). - Results: Under the framework of the International Cucurbit Genomics Initiative (ICuGI, http://www.icugi.org), an integrated genetic map has been constructed by merging data from eight independent mapping experiments using a genetically diverse array of parental lines. The consensus map spans 1150 cM across the 12 melon linkage groups and is composed of 1592 markers (640 SSRs, 330 SNPs, 252 AFLPs, 239 RFLPs, 89 RAPDs, 15 IMAs, 16 indels and 11 morphological traits) with a mean marker density of 0.72 cM/marker. One hundred and ninety-six of these markers (157 SSRs, 32 SNPs, 6 indels and 1 RAPD) were newly developed, mapped or provided by industry representatives as released markers, including 27 SNPs and 5 indels from genes involved in the organic acid metabolism and transport, and 58 EST-SSRs. Additionally, 85 of 822 SSR markers contributed by Syngenta Seeds were included in the integrated map. In addition, 370 QTL controlling 62 traits from 18 previously reported mapping experiments using genetically diverse parental genotypes were also integrated into the consensus map. Some QTL associated with economically important traits detected in separate studies mapped to similar genòmic positions. For example, independently identified QTL controlling fruit shape were mapped on similar genòmic positions, suggesting that such QTL are possibly responsible for the phenotypic variability observed for this trait in a broad array of melon germplasm. - Conclusions: Even though relatively unsaturated genetic maps in a diverse set of melon market types have been published, the integrated saturated map presented herein should be considered the initial reference map for melon. Most of the mapped markers contained in the reference map are polymorphic in diverse collection of germplasm, and thus are potentially transferrable to a broad array of genetic experimentation (e.g., integration of physical and genetic maps, colinearity analysis, map-based gene cloning, epistasis dissection, and marker-assisted selection).
publishDate 2011
dc.date.none.fl_str_mv 2
2011-01-01
2011
2011-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/180284
url https://ddd.uab.cat/record/180284
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 AGL2009/12698-C02-02
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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