Study of viscoelasticity and adhesion of human alveolar epithelial cells by atomic force microscopy. The importance of probe geometry

[eng] During the last century, cellular biology has been mostly assessed from the biochemical point of view: studying how biochemical stimuli modify the biochemical composition of living cells. However, most of the cells that form our body constantly exert or are subjected to mechanical forces. Musc...

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Detalles Bibliográficos
Autor: Rico Camps, Félix
Tipo de recurso: tesis doctoral
Estado:Versión publicada
Fecha de publicación:2006
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/36594
Acceso en línea:https://hdl.handle.net/2445/36594
http://www.tdx.cat/TDX-0301107-082508
http://hdl.handle.net/10803/1132
Access Level:acceso abierto
Palabra clave:Cèl·lules epitelials
Citologia
Nanotecnologia
Microscòpia de força atòmica
Epithelial cells
Cytology
Nanotechnology
Atomic force microscopy
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spelling Study of viscoelasticity and adhesion of human alveolar epithelial cells by atomic force microscopy. The importance of probe geometryRico Camps, FélixCèl·lules epitelialsCitologiaNanotecnologiaMicroscòpia de força atòmicaEpithelial cellsCytologyNanotechnologyAtomic force microscopy[eng] During the last century, cellular biology has been mostly assessed from the biochemical point of view: studying how biochemical stimuli modify the biochemical composition of living cells. However, most of the cells that form our body constantly exert or are subjected to mechanical forces. Muscle cells exert forces during contraction, vascular endothelial cells are subjected to shear forces due to blood stream, and pulmonary cells resist cyclic deformations due to spontaneous breathing. Therefore, it appears reasonable that mechanical forces play an important role in determining cell structure, composition, and function. The continuous increase in works relating mechanical properties of cells with cellular function has shown that mechanics are as important as biochemistry at the cellular level. Even the high development of nanotechnology, techniques such as atomic force microscopy (AFM) still present important limitations when applied to biological systems. The general aim of this thesis was to improve and apply AFM methods to the measurement of mechanical properties of living cells, laying especial emphasis on the probe geometry. The studies presented in this thesis are part of the work I carried out during the last four years in the Biophysics and Bioengineering Unit at the University of Barcelona School of Medicine. The thesis can be divided into four main sections: Introduction, Aims, Experimental studies, and Conclusions. The introduction (Chapter 1) attempts to make a brief review of cellular mechanics. The specific aims are presented in Chapter 2. The first work (Chapter 3) describes the design and set-up of an AFM based system to measure the mechanical properties (viscoelasticity and adhesion) of living cells under physiological conditions. The second work (Chapter 4) describes the development and validation of a blunted pyramidal elastic model used to determine the viscoelastic properties of soft gels and living cells. The third study (Chapter 5) validates the use of FIB modified flat-ended cylindrical tips to study the mechanical properties of biopolymer gels and living cells. The last work (Chapter 6) applies the modified cylindrical cantilevers and the developed AFM system to measure elastic and adhesive properties of living cells under inflammatory conditions.Universitat de BarcelonaNavajas Navarro, DanielUniversitat de Barcelona. Departament de Ciències Fisiològiques I2006info:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/36594http://www.tdx.cat/TDX-0301107-082508http://hdl.handle.net/10803/1132Tesis Doctorals - Departament - Ciències Fisiològiques Ireponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglés(c) Rico Camps, 2006info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/365942026-05-27T06:46:51Z
dc.title.none.fl_str_mv Study of viscoelasticity and adhesion of human alveolar epithelial cells by atomic force microscopy. The importance of probe geometry
title Study of viscoelasticity and adhesion of human alveolar epithelial cells by atomic force microscopy. The importance of probe geometry
spellingShingle Study of viscoelasticity and adhesion of human alveolar epithelial cells by atomic force microscopy. The importance of probe geometry
Rico Camps, Félix
Cèl·lules epitelials
Citologia
Nanotecnologia
Microscòpia de força atòmica
Epithelial cells
Cytology
Nanotechnology
Atomic force microscopy
title_short Study of viscoelasticity and adhesion of human alveolar epithelial cells by atomic force microscopy. The importance of probe geometry
title_full Study of viscoelasticity and adhesion of human alveolar epithelial cells by atomic force microscopy. The importance of probe geometry
title_fullStr Study of viscoelasticity and adhesion of human alveolar epithelial cells by atomic force microscopy. The importance of probe geometry
title_full_unstemmed Study of viscoelasticity and adhesion of human alveolar epithelial cells by atomic force microscopy. The importance of probe geometry
title_sort Study of viscoelasticity and adhesion of human alveolar epithelial cells by atomic force microscopy. The importance of probe geometry
dc.creator.none.fl_str_mv Rico Camps, Félix
author Rico Camps, Félix
author_facet Rico Camps, Félix
author_role author
dc.contributor.none.fl_str_mv Navajas Navarro, Daniel
Universitat de Barcelona. Departament de Ciències Fisiològiques I
dc.subject.none.fl_str_mv Cèl·lules epitelials
Citologia
Nanotecnologia
Microscòpia de força atòmica
Epithelial cells
Cytology
Nanotechnology
Atomic force microscopy
topic Cèl·lules epitelials
Citologia
Nanotecnologia
Microscòpia de força atòmica
Epithelial cells
Cytology
Nanotechnology
Atomic force microscopy
description [eng] During the last century, cellular biology has been mostly assessed from the biochemical point of view: studying how biochemical stimuli modify the biochemical composition of living cells. However, most of the cells that form our body constantly exert or are subjected to mechanical forces. Muscle cells exert forces during contraction, vascular endothelial cells are subjected to shear forces due to blood stream, and pulmonary cells resist cyclic deformations due to spontaneous breathing. Therefore, it appears reasonable that mechanical forces play an important role in determining cell structure, composition, and function. The continuous increase in works relating mechanical properties of cells with cellular function has shown that mechanics are as important as biochemistry at the cellular level. Even the high development of nanotechnology, techniques such as atomic force microscopy (AFM) still present important limitations when applied to biological systems. The general aim of this thesis was to improve and apply AFM methods to the measurement of mechanical properties of living cells, laying especial emphasis on the probe geometry. The studies presented in this thesis are part of the work I carried out during the last four years in the Biophysics and Bioengineering Unit at the University of Barcelona School of Medicine. The thesis can be divided into four main sections: Introduction, Aims, Experimental studies, and Conclusions. The introduction (Chapter 1) attempts to make a brief review of cellular mechanics. The specific aims are presented in Chapter 2. The first work (Chapter 3) describes the design and set-up of an AFM based system to measure the mechanical properties (viscoelasticity and adhesion) of living cells under physiological conditions. The second work (Chapter 4) describes the development and validation of a blunted pyramidal elastic model used to determine the viscoelastic properties of soft gels and living cells. The third study (Chapter 5) validates the use of FIB modified flat-ended cylindrical tips to study the mechanical properties of biopolymer gels and living cells. The last work (Chapter 6) applies the modified cylindrical cantilevers and the developed AFM system to measure elastic and adhesive properties of living cells under inflammatory conditions.
publishDate 2006
dc.date.none.fl_str_mv 2006
dc.type.none.fl_str_mv info:eu-repo/semantics/doctoralThesis
info:eu-repo/semantics/publishedVersion
format doctoralThesis
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/36594
http://www.tdx.cat/TDX-0301107-082508
http://hdl.handle.net/10803/1132
url https://hdl.handle.net/2445/36594
http://www.tdx.cat/TDX-0301107-082508
http://hdl.handle.net/10803/1132
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv (c) Rico Camps, 2006
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Rico Camps, 2006
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universitat de Barcelona
publisher.none.fl_str_mv Universitat de Barcelona
dc.source.none.fl_str_mv Tesis Doctorals - Departament - Ciències Fisiològiques I
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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