The ectD gene, which is involved in the synthesis of the compatible solute hydroxyectoine, is essential for thermoprotection of the halophilic bacterium Chromohalobacter salexigens

The halophilic bacterium Chromohalobacter salexigens synthesizes and accumulates compatible solutes in response to salt and temperature stress. 13C-nuclear magnetic resonance analysis of cells grown in minimal medium at the limiting temperature of 45°C revealed the presence of hydroxyectoine, ectoin...

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Autores: Nieto Gutiérrez, Joaquín José, García Estepa, Raúl, Argandoña Bertrán, Montserrat, Reina Bueno, Mercedes, Capote Maínez, María Nieves, Iglesias Guerra, Fernando, Vargas Macías, Carmen
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2006
País:España
Recursos:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/38259
Acesso em linha:http://hdl.handle.net/11441/38259
https://doi.org/10.1128/JB.00136-06
Access Level:acceso abierto
Palavra-chave:Diaminobutyric acid
Ectoine
Ectoine hydroxylase
Glutamic acid
Hydroxyectoine
N gamma acetyldiaminobutyric acid
Oxygenase
Sodium chloride
Trehalose
Unclassified drug
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spelling The ectD gene, which is involved in the synthesis of the compatible solute hydroxyectoine, is essential for thermoprotection of the halophilic bacterium Chromohalobacter salexigensNieto Gutiérrez, Joaquín JoséGarcía Estepa, RaúlArgandoña Bertrán, MontserratReina Bueno, MercedesCapote Maínez, María NievesIglesias Guerra, FernandoVargas Macías, CarmenDiaminobutyric acidEctoineEctoine hydroxylaseGlutamic acidHydroxyectoineN gamma acetyldiaminobutyric acidOxygenaseSodium chlorideTrehaloseUnclassified drugThe halophilic bacterium Chromohalobacter salexigens synthesizes and accumulates compatible solutes in response to salt and temperature stress. 13C-nuclear magnetic resonance analysis of cells grown in minimal medium at the limiting temperature of 45°C revealed the presence of hydroxyectoine, ectoine, glutamate, trehalose (not present in cells grown at 37°C), and the ectoine precursor, N')'-acetyldiaminobutyric acid. High-performance liquid chromatography analyses showed that the levels of ectoine and hydroxyectoine were maximal during the stationary phase of growth. Accumulation of hydroxyectoine was up-regulated by salinity and temperature, whereas accumulation of ectoine was up-regulated by salinity and down-regulated by tem- perature. The ectD gene, which is involved in the conversion of ectoine to hydroxyectoine, was isolated as part of a DNA region that also contains a gene whose product belongs to the AraC-XylS family of transcriptional activators. Orthologs of ectD were found within the sequenced genomes of members of the proteobacteria, firmicutes, and actinobacteria, and their products were grouped into the ectoine hydroxylase subfamily, which was shown to belong to the superfamily of Fe(II)- and 2-oxoglutarate-dependent oxygenases. Analysis of the ectoine and hydroxyectoine contents of an ectABC ectD mutant strain fed with 1 mM ectoine or hydroxyectoine demonstrated that ectD is required for the main ectoine hydroxylase activity in C. salexigens. Although in minimal medium at 37°C the wild-type strain grew with 0.5 to 3.0 M NaCl, with optimal growth at 1.5 M NaCl, at 45°C it could not cope with the lowest (0.75 M NaCl) or the highest (3.0 M NaCl) salinity, and it grew optimally at 2.5 M NaCl. The ectD mutation caused a growth defect at 45°C in minimal medium with 1.5 to 2.5 M NaCl, but it did not affect growth at 37°C at any salinity tested. With 2.5 M NaCl, the ectD mutant synthesized 38% (at 37°C) and 15% (at 45°C) of the hydroxyectoine produced by the wild-type strain. All of these data reveal that hydroxyectoine synthesis mediated by the ectD gene is thermoregulated and essential for thermoprotection of C. salexigens.American Society for MicrobiologyMicrobiología y ParasitologíaQuímica Orgánica y Farmacéutica2006info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/11441/38259https://doi.org/10.1128/JB.00136-06reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésJournal of Bacteriology, 188, 3774-3784http://dx.doi.org/10.1128/JB.00136-06info:eu-repo/semantics/openAccessoai:idus.us.es:11441/382592026-06-17T12:51:07Z
dc.title.none.fl_str_mv The ectD gene, which is involved in the synthesis of the compatible solute hydroxyectoine, is essential for thermoprotection of the halophilic bacterium Chromohalobacter salexigens
title The ectD gene, which is involved in the synthesis of the compatible solute hydroxyectoine, is essential for thermoprotection of the halophilic bacterium Chromohalobacter salexigens
spellingShingle The ectD gene, which is involved in the synthesis of the compatible solute hydroxyectoine, is essential for thermoprotection of the halophilic bacterium Chromohalobacter salexigens
Nieto Gutiérrez, Joaquín José
Diaminobutyric acid
Ectoine
Ectoine hydroxylase
Glutamic acid
Hydroxyectoine
N gamma acetyldiaminobutyric acid
Oxygenase
Sodium chloride
Trehalose
Unclassified drug
title_short The ectD gene, which is involved in the synthesis of the compatible solute hydroxyectoine, is essential for thermoprotection of the halophilic bacterium Chromohalobacter salexigens
title_full The ectD gene, which is involved in the synthesis of the compatible solute hydroxyectoine, is essential for thermoprotection of the halophilic bacterium Chromohalobacter salexigens
title_fullStr The ectD gene, which is involved in the synthesis of the compatible solute hydroxyectoine, is essential for thermoprotection of the halophilic bacterium Chromohalobacter salexigens
title_full_unstemmed The ectD gene, which is involved in the synthesis of the compatible solute hydroxyectoine, is essential for thermoprotection of the halophilic bacterium Chromohalobacter salexigens
title_sort The ectD gene, which is involved in the synthesis of the compatible solute hydroxyectoine, is essential for thermoprotection of the halophilic bacterium Chromohalobacter salexigens
dc.creator.none.fl_str_mv Nieto Gutiérrez, Joaquín José
García Estepa, Raúl
Argandoña Bertrán, Montserrat
Reina Bueno, Mercedes
Capote Maínez, María Nieves
Iglesias Guerra, Fernando
Vargas Macías, Carmen
author Nieto Gutiérrez, Joaquín José
author_facet Nieto Gutiérrez, Joaquín José
García Estepa, Raúl
Argandoña Bertrán, Montserrat
Reina Bueno, Mercedes
Capote Maínez, María Nieves
Iglesias Guerra, Fernando
Vargas Macías, Carmen
author_role author
author2 García Estepa, Raúl
Argandoña Bertrán, Montserrat
Reina Bueno, Mercedes
Capote Maínez, María Nieves
Iglesias Guerra, Fernando
Vargas Macías, Carmen
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Microbiología y Parasitología
Química Orgánica y Farmacéutica
dc.subject.none.fl_str_mv Diaminobutyric acid
Ectoine
Ectoine hydroxylase
Glutamic acid
Hydroxyectoine
N gamma acetyldiaminobutyric acid
Oxygenase
Sodium chloride
Trehalose
Unclassified drug
topic Diaminobutyric acid
Ectoine
Ectoine hydroxylase
Glutamic acid
Hydroxyectoine
N gamma acetyldiaminobutyric acid
Oxygenase
Sodium chloride
Trehalose
Unclassified drug
description The halophilic bacterium Chromohalobacter salexigens synthesizes and accumulates compatible solutes in response to salt and temperature stress. 13C-nuclear magnetic resonance analysis of cells grown in minimal medium at the limiting temperature of 45°C revealed the presence of hydroxyectoine, ectoine, glutamate, trehalose (not present in cells grown at 37°C), and the ectoine precursor, N')'-acetyldiaminobutyric acid. High-performance liquid chromatography analyses showed that the levels of ectoine and hydroxyectoine were maximal during the stationary phase of growth. Accumulation of hydroxyectoine was up-regulated by salinity and temperature, whereas accumulation of ectoine was up-regulated by salinity and down-regulated by tem- perature. The ectD gene, which is involved in the conversion of ectoine to hydroxyectoine, was isolated as part of a DNA region that also contains a gene whose product belongs to the AraC-XylS family of transcriptional activators. Orthologs of ectD were found within the sequenced genomes of members of the proteobacteria, firmicutes, and actinobacteria, and their products were grouped into the ectoine hydroxylase subfamily, which was shown to belong to the superfamily of Fe(II)- and 2-oxoglutarate-dependent oxygenases. Analysis of the ectoine and hydroxyectoine contents of an ectABC ectD mutant strain fed with 1 mM ectoine or hydroxyectoine demonstrated that ectD is required for the main ectoine hydroxylase activity in C. salexigens. Although in minimal medium at 37°C the wild-type strain grew with 0.5 to 3.0 M NaCl, with optimal growth at 1.5 M NaCl, at 45°C it could not cope with the lowest (0.75 M NaCl) or the highest (3.0 M NaCl) salinity, and it grew optimally at 2.5 M NaCl. The ectD mutation caused a growth defect at 45°C in minimal medium with 1.5 to 2.5 M NaCl, but it did not affect growth at 37°C at any salinity tested. With 2.5 M NaCl, the ectD mutant synthesized 38% (at 37°C) and 15% (at 45°C) of the hydroxyectoine produced by the wild-type strain. All of these data reveal that hydroxyectoine synthesis mediated by the ectD gene is thermoregulated and essential for thermoprotection of C. salexigens.
publishDate 2006
dc.date.none.fl_str_mv 2006
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11441/38259
https://doi.org/10.1128/JB.00136-06
url http://hdl.handle.net/11441/38259
https://doi.org/10.1128/JB.00136-06
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Journal of Bacteriology, 188, 3774-3784
http://dx.doi.org/10.1128/JB.00136-06
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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