H3 lysine 4 methylation is required for full activation of genes involved in α-ketoglutarate availability in the nucleus of yeast cells after diauxic shift

We show that in S. cerevisiae the metabolic diauxic shift is associated with a H3 lysine 4 tri-methylation (H3K4me3) increase which involves a significant fraction of transcriptionally induced genes which are required for the metabolic changes, suggesting a role for histone methylation in their tran...

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Authors: Di Nisio, Elena, Danovska, Svetlana, Condemi, Livia, Cirigliano, Angela, Rinaldi, Teresa, Licursi, Valerio, Negri, Rodolfo
Format: article
Status:Published version
Publication Date:2023
Country:España
Institution:Universitat Pompeu Fabra
Repository:Repositorio Digital de la UPF
OAI Identifier:oai:repositori.upf.edu:10230/57315
Online Access:http://hdl.handle.net/10230/57315
http://dx.doi.org/10.3390/metabo13040507
Access Level:Open access
Keyword:H3K4 tri-methylation
Diauxic shift
Transcriptional regulation
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spelling H3 lysine 4 methylation is required for full activation of genes involved in α-ketoglutarate availability in the nucleus of yeast cells after diauxic shiftDi Nisio, ElenaDanovska, SvetlanaCondemi, LiviaCirigliano, AngelaRinaldi, TeresaLicursi, ValerioNegri, RodolfoH3K4 tri-methylationDiauxic shiftTranscriptional regulationWe show that in S. cerevisiae the metabolic diauxic shift is associated with a H3 lysine 4 tri-methylation (H3K4me3) increase which involves a significant fraction of transcriptionally induced genes which are required for the metabolic changes, suggesting a role for histone methylation in their transcriptional regulation. We show that histone H3K4me3 around the start site correlates with transcriptional induction in some of these genes. Among the methylation-induced genes are IDP2 and ODC1, which regulate the nuclear availability of α-ketoglutarate, which, as a cofactor for Jhd2 demethylase, regulates H3K4 tri-methylation. We propose that this feedback circuit could be used to regulate the nuclear α-ketoglutarate pool concentration. We also show that yeast cells adapt to the absence of Jhd2 by decreasing Set1 methylation activity.MDPI202320232023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/57315http://dx.doi.org/10.3390/metabo13040507reponame:Repositorio Digital de la UPFinstname:Universitat Pompeu FabraInglésMetabolites. 2023 Mar 31;13(4):507© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repositori.upf.edu:10230/573152026-06-12T07:21:37Z
dc.title.none.fl_str_mv H3 lysine 4 methylation is required for full activation of genes involved in α-ketoglutarate availability in the nucleus of yeast cells after diauxic shift
title H3 lysine 4 methylation is required for full activation of genes involved in α-ketoglutarate availability in the nucleus of yeast cells after diauxic shift
spellingShingle H3 lysine 4 methylation is required for full activation of genes involved in α-ketoglutarate availability in the nucleus of yeast cells after diauxic shift
Di Nisio, Elena
H3K4 tri-methylation
Diauxic shift
Transcriptional regulation
title_short H3 lysine 4 methylation is required for full activation of genes involved in α-ketoglutarate availability in the nucleus of yeast cells after diauxic shift
title_full H3 lysine 4 methylation is required for full activation of genes involved in α-ketoglutarate availability in the nucleus of yeast cells after diauxic shift
title_fullStr H3 lysine 4 methylation is required for full activation of genes involved in α-ketoglutarate availability in the nucleus of yeast cells after diauxic shift
title_full_unstemmed H3 lysine 4 methylation is required for full activation of genes involved in α-ketoglutarate availability in the nucleus of yeast cells after diauxic shift
title_sort H3 lysine 4 methylation is required for full activation of genes involved in α-ketoglutarate availability in the nucleus of yeast cells after diauxic shift
dc.creator.none.fl_str_mv Di Nisio, Elena
Danovska, Svetlana
Condemi, Livia
Cirigliano, Angela
Rinaldi, Teresa
Licursi, Valerio
Negri, Rodolfo
author Di Nisio, Elena
author_facet Di Nisio, Elena
Danovska, Svetlana
Condemi, Livia
Cirigliano, Angela
Rinaldi, Teresa
Licursi, Valerio
Negri, Rodolfo
author_role author
author2 Danovska, Svetlana
Condemi, Livia
Cirigliano, Angela
Rinaldi, Teresa
Licursi, Valerio
Negri, Rodolfo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv H3K4 tri-methylation
Diauxic shift
Transcriptional regulation
topic H3K4 tri-methylation
Diauxic shift
Transcriptional regulation
description We show that in S. cerevisiae the metabolic diauxic shift is associated with a H3 lysine 4 tri-methylation (H3K4me3) increase which involves a significant fraction of transcriptionally induced genes which are required for the metabolic changes, suggesting a role for histone methylation in their transcriptional regulation. We show that histone H3K4me3 around the start site correlates with transcriptional induction in some of these genes. Among the methylation-induced genes are IDP2 and ODC1, which regulate the nuclear availability of α-ketoglutarate, which, as a cofactor for Jhd2 demethylase, regulates H3K4 tri-methylation. We propose that this feedback circuit could be used to regulate the nuclear α-ketoglutarate pool concentration. We also show that yeast cells adapt to the absence of Jhd2 by decreasing Set1 methylation activity.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/57315
http://dx.doi.org/10.3390/metabo13040507
url http://hdl.handle.net/10230/57315
http://dx.doi.org/10.3390/metabo13040507
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Metabolites. 2023 Mar 31;13(4):507
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositorio Digital de la UPF
instname:Universitat Pompeu Fabra
instname_str Universitat Pompeu Fabra
reponame_str Repositorio Digital de la UPF
collection Repositorio Digital de la UPF
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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