Modulation and Protection Effects of Antioxidant Compounds against Oxidant Induced Developmental Toxicity in Zebrafish

The antioxidant effect of compounds is regularly evaluated by in vitro assays that do not have the capability to predict in vivo protective activity or to determine their underlying mechanisms of action. The aim of this study was to develop an experimental system to evaluate the in vivo protective e...

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Autores: Boix Sabrià, Núria, Teixidó Condomines, Elisabet, Piqué Benages, Maria Esther, Llobet Mallafré, Joan M. (Joan Maria), Gómez Catalán, Jesús
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/170934
Acceso en línea:https://hdl.handle.net/2445/170934
Access Level:acceso abierto
Palabra clave:Estrès oxidatiu
Antioxidants
Toxicologia
Peix zebra
Oxidative stress
Toxicology
Zebra danio
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spelling Modulation and Protection Effects of Antioxidant Compounds against Oxidant Induced Developmental Toxicity in ZebrafishBoix Sabrià, NúriaTeixidó Condomines, ElisabetPiqué Benages, Maria EstherLlobet Mallafré, Joan M. (Joan Maria)Gómez Catalán, JesúsEstrès oxidatiuAntioxidantsToxicologiaPeix zebraOxidative stressAntioxidantsToxicologyZebra danioThe antioxidant effect of compounds is regularly evaluated by in vitro assays that do not have the capability to predict in vivo protective activity or to determine their underlying mechanisms of action. The aim of this study was to develop an experimental system to evaluate the in vivo protective effects of different antioxidant compounds, based on the zebrafish embryo test. Zebrafish embryos were exposed to tert-butyl hydroperoxide (tBOOH), tetrachlorohydroquinone (TCHQ) and lipopolysaccharides from Escherichia coli (LPS), chemicals that are known inducers of oxidative stress in zebrafish. The developmental toxic effects (lethality or dysmorphogenesis) induced by these chemicals were modulated with n-acetyl l-cysteine and Nω-nitro l-arginine methyl ester hydrochloride, dimethyl maleate and dl-buthionine sulfoximine in order to validate the oxidant mechanism of oxidative stress inducers. The oxidant effects of tBOOH, TCHQ, and LPS were confirmed by the determination of significant differences in the comparison between the concentration-response curves of the oxidative stress inducers and of the modulators of antioxidant status. This concept was also applied to the study of the effects of well-known antioxidants, such as vitamin E, quercetin, and lipoic acid. Our results confirm the zebrafish model as an in vivo useful tool to test the protective effects of antioxidant compounds.MDPI2020202020202020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/2445/170934Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.3390/antiox9080721Antioxidants, 2020, vol. 9, p. 721https://doi.org/10.3390/antiox9080721cc-by (c) Boix Sabrià, Núria et al., 2020http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1709342026-05-29T05:05:01Z
dc.title.none.fl_str_mv Modulation and Protection Effects of Antioxidant Compounds against Oxidant Induced Developmental Toxicity in Zebrafish
title Modulation and Protection Effects of Antioxidant Compounds against Oxidant Induced Developmental Toxicity in Zebrafish
spellingShingle Modulation and Protection Effects of Antioxidant Compounds against Oxidant Induced Developmental Toxicity in Zebrafish
Boix Sabrià, Núria
Estrès oxidatiu
Antioxidants
Toxicologia
Peix zebra
Oxidative stress
Antioxidants
Toxicology
Zebra danio
title_short Modulation and Protection Effects of Antioxidant Compounds against Oxidant Induced Developmental Toxicity in Zebrafish
title_full Modulation and Protection Effects of Antioxidant Compounds against Oxidant Induced Developmental Toxicity in Zebrafish
title_fullStr Modulation and Protection Effects of Antioxidant Compounds against Oxidant Induced Developmental Toxicity in Zebrafish
title_full_unstemmed Modulation and Protection Effects of Antioxidant Compounds against Oxidant Induced Developmental Toxicity in Zebrafish
title_sort Modulation and Protection Effects of Antioxidant Compounds against Oxidant Induced Developmental Toxicity in Zebrafish
dc.creator.none.fl_str_mv Boix Sabrià, Núria
Teixidó Condomines, Elisabet
Piqué Benages, Maria Esther
Llobet Mallafré, Joan M. (Joan Maria)
Gómez Catalán, Jesús
author Boix Sabrià, Núria
author_facet Boix Sabrià, Núria
Teixidó Condomines, Elisabet
Piqué Benages, Maria Esther
Llobet Mallafré, Joan M. (Joan Maria)
Gómez Catalán, Jesús
author_role author
author2 Teixidó Condomines, Elisabet
Piqué Benages, Maria Esther
Llobet Mallafré, Joan M. (Joan Maria)
Gómez Catalán, Jesús
author2_role author
author
author
author
dc.subject.none.fl_str_mv Estrès oxidatiu
Antioxidants
Toxicologia
Peix zebra
Oxidative stress
Antioxidants
Toxicology
Zebra danio
topic Estrès oxidatiu
Antioxidants
Toxicologia
Peix zebra
Oxidative stress
Antioxidants
Toxicology
Zebra danio
description The antioxidant effect of compounds is regularly evaluated by in vitro assays that do not have the capability to predict in vivo protective activity or to determine their underlying mechanisms of action. The aim of this study was to develop an experimental system to evaluate the in vivo protective effects of different antioxidant compounds, based on the zebrafish embryo test. Zebrafish embryos were exposed to tert-butyl hydroperoxide (tBOOH), tetrachlorohydroquinone (TCHQ) and lipopolysaccharides from Escherichia coli (LPS), chemicals that are known inducers of oxidative stress in zebrafish. The developmental toxic effects (lethality or dysmorphogenesis) induced by these chemicals were modulated with n-acetyl l-cysteine and Nω-nitro l-arginine methyl ester hydrochloride, dimethyl maleate and dl-buthionine sulfoximine in order to validate the oxidant mechanism of oxidative stress inducers. The oxidant effects of tBOOH, TCHQ, and LPS were confirmed by the determination of significant differences in the comparison between the concentration-response curves of the oxidative stress inducers and of the modulators of antioxidant status. This concept was also applied to the study of the effects of well-known antioxidants, such as vitamin E, quercetin, and lipoic acid. Our results confirm the zebrafish model as an in vivo useful tool to test the protective effects of antioxidant compounds.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/170934
url https://hdl.handle.net/2445/170934
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/antiox9080721
Antioxidants, 2020, vol. 9, p. 721
https://doi.org/10.3390/antiox9080721
dc.rights.none.fl_str_mv cc-by (c) Boix Sabrià, Núria et al., 2020
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Boix Sabrià, Núria et al., 2020
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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