Digestive and metabolic consequences of on-growing greater amberjack (Seriola dumerili) juveniles at different temperatures. In-vivo and ex-vivo assessment

Aiming to elucidate the effects of temperature on different aspects of the fish digestive physiology that may affect the growth, we examined, firstly, variations in growth performance, feed intake, digestive enzyme activities, expression of transport related genes, metabolites in plasma and liver, a...

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Autores: Navarro-Guillén, Carmen, Perera, Erick, Pérez-Hilario, Desiderio, Martos-Sitcha, Juan Antonio, Molina-Roque, Luis, Gregório, Silvia Filipa, Fonseca, Filomena, Fuentes, Juan, Yúfera, Manuel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/378408
Acceso en línea:http://hdl.handle.net/10261/378408
https://api.elsevier.com/content/abstract/scopus_id/85212230893
Access Level:acceso abierto
Palabra clave:Digestion
Greater amberjack
Intestinal integrity
Temperature
Ussing chambers
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network_name_str España
repository_id_str
dc.title.none.fl_str_mv Digestive and metabolic consequences of on-growing greater amberjack (Seriola dumerili) juveniles at different temperatures. In-vivo and ex-vivo assessment
title Digestive and metabolic consequences of on-growing greater amberjack (Seriola dumerili) juveniles at different temperatures. In-vivo and ex-vivo assessment
spellingShingle Digestive and metabolic consequences of on-growing greater amberjack (Seriola dumerili) juveniles at different temperatures. In-vivo and ex-vivo assessment
Navarro-Guillén, Carmen
Digestion
Greater amberjack
Intestinal integrity
Temperature
Ussing chambers
title_short Digestive and metabolic consequences of on-growing greater amberjack (Seriola dumerili) juveniles at different temperatures. In-vivo and ex-vivo assessment
title_full Digestive and metabolic consequences of on-growing greater amberjack (Seriola dumerili) juveniles at different temperatures. In-vivo and ex-vivo assessment
title_fullStr Digestive and metabolic consequences of on-growing greater amberjack (Seriola dumerili) juveniles at different temperatures. In-vivo and ex-vivo assessment
title_full_unstemmed Digestive and metabolic consequences of on-growing greater amberjack (Seriola dumerili) juveniles at different temperatures. In-vivo and ex-vivo assessment
title_sort Digestive and metabolic consequences of on-growing greater amberjack (Seriola dumerili) juveniles at different temperatures. In-vivo and ex-vivo assessment
dc.creator.none.fl_str_mv Navarro-Guillén, Carmen
Perera, Erick
Pérez-Hilario, Desiderio
Martos-Sitcha, Juan Antonio
Molina-Roque, Luis
Gregório, Silvia Filipa
Fonseca, Filomena
Fuentes, Juan
Yúfera, Manuel
author Navarro-Guillén, Carmen
author_facet Navarro-Guillén, Carmen
Perera, Erick
Pérez-Hilario, Desiderio
Martos-Sitcha, Juan Antonio
Molina-Roque, Luis
Gregório, Silvia Filipa
Fonseca, Filomena
Fuentes, Juan
Yúfera, Manuel
author_role author
author2 Perera, Erick
Pérez-Hilario, Desiderio
Martos-Sitcha, Juan Antonio
Molina-Roque, Luis
Gregório, Silvia Filipa
Fonseca, Filomena
Fuentes, Juan
Yúfera, Manuel
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
European Commission
Junta de Andalucía
Fundação para a Ciência e a Tecnologia (Portugal)
Navarro-Guillén, Carmen [0000-0001-5490-0328]
Perera, Erick [0000-0001-6108-1340]
Martos-Sitcha, Juan Antonio [0000-0002-0151-7250]
Molina-Roque, Luis [0000-0001-9388-7330]
Fonseca, Filomena [0000-0003-3824-0459]
Fuentes, Juan [0000-0003-0430-8734]
Yúfera, Manuel [0000-0003-2999-4611]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Digestion
Greater amberjack
Intestinal integrity
Temperature
Ussing chambers
topic Digestion
Greater amberjack
Intestinal integrity
Temperature
Ussing chambers
description Aiming to elucidate the effects of temperature on different aspects of the fish digestive physiology that may affect the growth, we examined, firstly, variations in growth performance, feed intake, digestive enzyme activities, expression of transport related genes, metabolites in plasma and liver, and oxidative stress response in juveniles of greater amberjack (Seriola dumerili) growing during two months at 18, 22 and 26 °C of water temperature. Secondly, we analysed the epithelial function of the intestinal mucosa by electrophysiological ex-vivo experiments. As expected, body growth increased with increasing temperature in the tested range. Highest relative feed intake was observed at 26 °C, but the food conversion ratio was the same at 22 and 26 °C and less favourable at 18 °C. Digestive proteases activities were similar at 22 and 26 °C, while an evident effect of temperature was observed on lipids digestive capacity, being lipase activity undetectable at 18 °C. Electrophysiological assays revealed a relationship between temperature and intestinal mucosa plasticity. Temperature increase promoted epithelial functionality through higher tissue resistance and short-circuit current in mid-intestine at 26 °C, as well as better electrogenic amino acids transport. On the other hand, mRNA expression of peptide transporters tended to be higher in fish that grew at 18 °C, probably to reinforce the transport capacity. Plasma circulating levels of metabolites demonstrated higher energy and protein mobilization with the increasing temperature, where a hypometabolic state was denoted by lower cortisol levels at 18 °C together with an apparent switch from lipids to carbohydrate usage as energy source and increased oxidative stress in the liver at the lowest temperature. Altogether indicates that the tested temperatures are within the tolerance range for the species, although 22 and 26 °C appear as optimal temperatures for on-growing greater amberjack juveniles. Changes in nutrient digestion and absorption induced by temperature are related with both hydrolytic activity and remodelling of the intestinal mucosa. Impairing growth capacity and initial evidences of welfare compromise were observed at 18 °C.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/378408
https://api.elsevier.com/content/abstract/scopus_id/85212230893
url http://hdl.handle.net/10261/378408
https://api.elsevier.com/content/abstract/scopus_id/85212230893
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096134-B-I00
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1016/j.aquaculture.2024.742011
https://doi.org/10.1016/j.aquaculture.2024.742011
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier BV
publisher.none.fl_str_mv Elsevier BV
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
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repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Digestive and metabolic consequences of on-growing greater amberjack (Seriola dumerili) juveniles at different temperatures. In-vivo and ex-vivo assessmentNavarro-Guillén, CarmenPerera, ErickPérez-Hilario, DesiderioMartos-Sitcha, Juan AntonioMolina-Roque, LuisGregório, Silvia FilipaFonseca, FilomenaFuentes, JuanYúfera, ManuelDigestionGreater amberjackIntestinal integrityTemperatureUssing chambersAiming to elucidate the effects of temperature on different aspects of the fish digestive physiology that may affect the growth, we examined, firstly, variations in growth performance, feed intake, digestive enzyme activities, expression of transport related genes, metabolites in plasma and liver, and oxidative stress response in juveniles of greater amberjack (Seriola dumerili) growing during two months at 18, 22 and 26 °C of water temperature. Secondly, we analysed the epithelial function of the intestinal mucosa by electrophysiological ex-vivo experiments. As expected, body growth increased with increasing temperature in the tested range. Highest relative feed intake was observed at 26 °C, but the food conversion ratio was the same at 22 and 26 °C and less favourable at 18 °C. Digestive proteases activities were similar at 22 and 26 °C, while an evident effect of temperature was observed on lipids digestive capacity, being lipase activity undetectable at 18 °C. Electrophysiological assays revealed a relationship between temperature and intestinal mucosa plasticity. Temperature increase promoted epithelial functionality through higher tissue resistance and short-circuit current in mid-intestine at 26 °C, as well as better electrogenic amino acids transport. On the other hand, mRNA expression of peptide transporters tended to be higher in fish that grew at 18 °C, probably to reinforce the transport capacity. Plasma circulating levels of metabolites demonstrated higher energy and protein mobilization with the increasing temperature, where a hypometabolic state was denoted by lower cortisol levels at 18 °C together with an apparent switch from lipids to carbohydrate usage as energy source and increased oxidative stress in the liver at the lowest temperature. Altogether indicates that the tested temperatures are within the tolerance range for the species, although 22 and 26 °C appear as optimal temperatures for on-growing greater amberjack juveniles. Changes in nutrient digestion and absorption induced by temperature are related with both hydrolytic activity and remodelling of the intestinal mucosa. Impairing growth capacity and initial evidences of welfare compromise were observed at 18 °C.This research was funded by the project THERMODIGEST, RTI2018-096134-B-I00 from the Spanish Ministry of Science, Innovation and Universities (MCIU), the State Research Agency (AEI) and the European Regional Development Fund (FEDER/ERDF) granted to MY. CN-G was supported by the researcher contract DOC_01203 funded by the Andalusian Plan for Research Development and Innovation (PAIDI 2020). CIMA receives Portuguese national funds from FCT - Foundation for Science and Technology through project UIDP/00350/2020 (https://doi.org/10.54499/UIDP/00350/2020 and https://doi.org/10.54499/UIDB/00350/2020). ARNET, Associate Laboratory, receives Portuguese national funds from FCT - Foundation for Science and Technology through project LA/P/0069/2020.Peer reviewedElsevier BVMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)European CommissionJunta de AndalucíaFundação para a Ciência e a Tecnologia (Portugal)Navarro-Guillén, Carmen [0000-0001-5490-0328]Perera, Erick [0000-0001-6108-1340]Martos-Sitcha, Juan Antonio [0000-0002-0151-7250]Molina-Roque, Luis [0000-0001-9388-7330]Fonseca, Filomena [0000-0003-3824-0459]Fuentes, Juan [0000-0003-0430-8734]Yúfera, Manuel [0000-0003-2999-4611]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/378408https://api.elsevier.com/content/abstract/scopus_id/85212230893reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096134-B-I00The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1016/j.aquaculture.2024.742011https://doi.org/10.1016/j.aquaculture.2024.742011Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3784082026-05-22T06:33:51Z
score 15.81155

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