Defining the transcriptional responses of Aspergillus nidulans to cation/alkaline pH stress and the role of the transcription factor SltA

18 p.-7 fig.

Detalles Bibliográficos
Autores: Picazo, Irene, Etxebeste, Oier, Requena, Elena, Garzia, Aitor, Espeso, Eduardo A.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/217973
Acceso en línea:http://hdl.handle.net/10261/217973
Access Level:acceso abierto
Palabra clave:Aspergillus nidulans
PacC/Rim101
SltA
Alkaline pH stress
Cation stress
Fungi
Signal transduction
Transcriptional control
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spelling Defining the transcriptional responses of Aspergillus nidulans to cation/alkaline pH stress and the role of the transcription factor SltAPicazo, IreneEtxebeste, OierRequena, ElenaGarzia, AitorEspeso, Eduardo A.Aspergillus nidulansPacC/Rim101SltAAlkaline pH stressCation stressFungiSignal transductionTranscriptional control18 p.-7 fig.Fungi have developed the ability to overcome extreme growth conditions and thrive in hostile environments. The model fungus Aspergillus nidulans tolerates, for example, ambient alkalinity up to pH 10 or molar concentrations of multiple cations. The ability to grow under alkaline pH or saline stress depends on the effective function of at least three regulatory pathways mediated by the zinc-finger transcription factor PacC, which mediates the ambient pH regulatory pathway, the calcineurin-dependent CrzA and the cation homeostasis responsive factor SltA. Using RNA sequencing, we determined the effect of external pH alkalinization or sodium stress on gene expression. The data show that each condition triggers transcriptional responses with a low degree of overlap. By sequencing the transcriptomes of the null mutant, the role of SltA in the above-mentioned homeostasis mechanisms was also studied. The results show that the transcriptional role of SltA is wider than initially expected and implies, for example, the positive control of the PacC-dependent ambient pH regulatory pathway. Overall, our data strongly suggest that the stress response pathways in fungi include some common but mostly exclusive constituents, and that there is a hierarchical relationship among the main regulators of stress response, with SltA controlling pacC expression, at least in A. nidulans.Work at CIB-CSIC was funded by MINECO (BFU2015-66806-R to E. A.E) and MICIU/AEI (RTI2018-094263-B-100) to E. A. E (both partially supported by FEDER, EU). Work at the UPV/EHU lab was funded by UPV/EHU grants PPGA19/08 and GIU19/014 to O. E and the Basque Government grant Elkartek19/72 (to Professor María Teresa Dueñas).I. P and E. R. held research contracts associated with grants RTI2018-094263-B-100 and BFU2015-66806-R,respectively.We acknowledge the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI) for enabling this open access publication through a Publish and Read deal with the Microbiology Society.Peer reviewedMicrobiology SocietyMinisterio de Economía y Competitividad (España)Ministerio de Ciencia, Innovación y Universidades (España)Eusko JaurlaritzaEtxebeste, Oier [0000-0002-9786-6091]Garzia, Aitor [0000-0002-6270-1369 ]Espeso, Eduardo A. [0000-0002-5873-6059]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/217973reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2015-66806-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094263-B-100https://doi.org/10.1099/mgen.0.000415Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2179732026-05-22T06:33:51Z
dc.title.none.fl_str_mv Defining the transcriptional responses of Aspergillus nidulans to cation/alkaline pH stress and the role of the transcription factor SltA
title Defining the transcriptional responses of Aspergillus nidulans to cation/alkaline pH stress and the role of the transcription factor SltA
spellingShingle Defining the transcriptional responses of Aspergillus nidulans to cation/alkaline pH stress and the role of the transcription factor SltA
Picazo, Irene
Aspergillus nidulans
PacC/Rim101
SltA
Alkaline pH stress
Cation stress
Fungi
Signal transduction
Transcriptional control
title_short Defining the transcriptional responses of Aspergillus nidulans to cation/alkaline pH stress and the role of the transcription factor SltA
title_full Defining the transcriptional responses of Aspergillus nidulans to cation/alkaline pH stress and the role of the transcription factor SltA
title_fullStr Defining the transcriptional responses of Aspergillus nidulans to cation/alkaline pH stress and the role of the transcription factor SltA
title_full_unstemmed Defining the transcriptional responses of Aspergillus nidulans to cation/alkaline pH stress and the role of the transcription factor SltA
title_sort Defining the transcriptional responses of Aspergillus nidulans to cation/alkaline pH stress and the role of the transcription factor SltA
dc.creator.none.fl_str_mv Picazo, Irene
Etxebeste, Oier
Requena, Elena
Garzia, Aitor
Espeso, Eduardo A.
author Picazo, Irene
author_facet Picazo, Irene
Etxebeste, Oier
Requena, Elena
Garzia, Aitor
Espeso, Eduardo A.
author_role author
author2 Etxebeste, Oier
Requena, Elena
Garzia, Aitor
Espeso, Eduardo A.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Eusko Jaurlaritza
Etxebeste, Oier [0000-0002-9786-6091]
Garzia, Aitor [0000-0002-6270-1369 ]
Espeso, Eduardo A. [0000-0002-5873-6059]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Aspergillus nidulans
PacC/Rim101
SltA
Alkaline pH stress
Cation stress
Fungi
Signal transduction
Transcriptional control
topic Aspergillus nidulans
PacC/Rim101
SltA
Alkaline pH stress
Cation stress
Fungi
Signal transduction
Transcriptional control
description 18 p.-7 fig.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/217973
url http://hdl.handle.net/10261/217973
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2015-66806-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094263-B-100
https://doi.org/10.1099/mgen.0.000415
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Microbiology Society
publisher.none.fl_str_mv Microbiology Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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