Changes in white adipose tissue metabolism induced by resveratrol in rats

Background: A remarkable range of biological functions have been ascribed to resveratrol. Recently, this polyphenol has been shown to have body fat lowering effects. The aim of the present study was to assess some of the potential underlying mechanisms of action which take place in adipose tissue. M...

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Autores: Alberdi, Goiuri, Rodríguez Rivera, Víctor Manuel, Miranda Gómez, Jonatan, Macarulla Arenaza, María Teresa, Arias Rueda, Noemí, Andrés Lacueva, Cristina, Portillo Baquedano, María Puy
Tipo de recurso: artículo
Fecha de publicación:2011
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/11583
Acceso en línea:http://hdl.handle.net/10810/11583
Access Level:acceso abierto
Palabra clave:fat mobilization
linoleic acid
body fat
adipocytes
mice
diet
obesity
SIRT1
modulation
expression
ENDOCRINOLOGY AND METABOLISM
NUTRITION AND DIETETICS
MEDICINE
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repository_id_str
spelling Changes in white adipose tissue metabolism induced by resveratrol in ratsAlberdi, GoiuriRodríguez Rivera, Víctor ManuelMiranda Gómez, JonatanMacarulla Arenaza, María TeresaArias Rueda, NoemíAndrés Lacueva, CristinaPortillo Baquedano, María Puyfat mobilizationlinoleic acidbody fatadipocytesmicedietobesitySIRT1modulationexpressionENDOCRINOLOGY AND METABOLISMNUTRITION AND DIETETICSMEDICINEBackground: A remarkable range of biological functions have been ascribed to resveratrol. Recently, this polyphenol has been shown to have body fat lowering effects. The aim of the present study was to assess some of the potential underlying mechanisms of action which take place in adipose tissue. Methods: Sixteen male Sprague-Dawley rats were randomly divided into two groups: control and treated with 30 mg resveratrol/kg body weight/d. All rats were fed an obesogenic diet and after six weeks of treatment white adipose tissues were dissected. Lipoprotein lipase activity was assessed by fluorimetry, acetyl-CoA carboxylase by radiometry, and malic enzyme, glucose-6P-dehydrogenase and fatty acid synthase by spectrophotometry. Gene expression levels of acetyl-CoA carboxylase, fatty acid synthase, lipoprotein lipase, hormone-sensitive lipase, adipose triglyceride lipase, PPAR-gamma, SREBP-1c and perilipin were assessed by Real time RT-PCR. The amount of resveratrol metabolites in adipose tissue was measured by chromatography. Results: There was no difference in the final body weight of the rats; however, adipose tissues were significantly decreased in the resveratrol-treated group. Resveratrol reduced the activity of lipogenic enzymes, as well as that of heparin-releasable lipoprotein lipase. Moreover, a significant reduction was induced by this polyphenol in hormone-sensitive lipase mRNA levels. No significant changes were observed in other genes. Total amount of resveratrol metabolites in adipose tissue was 2.66 +/- 0.55 nmol/g tissue. Conclusions: It can be proposed that the body fat-lowering effect of resveratrol is mediated, at least in part, by a reduction in fatty acid uptake from circulating triacylglycerols and also in de novo lipogenesis.This study was supported by grants from the Ministerio de Ciencia e Innovacion (AGL2008-1005-ALI and partially by the AGL2006-14228-C03-02), Instituto de Salud Carlos III (RETIC PREDIMED) and the Government of Pais Vasco (IT-386-10; CTP09/R5). G. Alberdi is a recipient of a doctoral fellowship from the Ministerio de Ciencia e Innovacion. Resveratrol was a generous gift from Monteloeder (Elche, Alicante, Spain).BioMed Central201420142011info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/11583reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoInglésinfo:eu-repo/grantAgreement/MICINN/AGL2008-1005-ALI/info:eu-repo/grantAgreement/MICINN/AGL2006-14228-C03-02/http://www.nutritionandmetabolism.com/content/8/1/29info:eu-repo/semantics/openAccess© 2011 Alberdi et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.oai:addi.ehu.eus:10810/115832026-06-18T09:23:17Z
dc.title.none.fl_str_mv Changes in white adipose tissue metabolism induced by resveratrol in rats
title Changes in white adipose tissue metabolism induced by resveratrol in rats
spellingShingle Changes in white adipose tissue metabolism induced by resveratrol in rats
Alberdi, Goiuri
fat mobilization
linoleic acid
body fat
adipocytes
mice
diet
obesity
SIRT1
modulation
expression
ENDOCRINOLOGY AND METABOLISM
NUTRITION AND DIETETICS
MEDICINE
title_short Changes in white adipose tissue metabolism induced by resveratrol in rats
title_full Changes in white adipose tissue metabolism induced by resveratrol in rats
title_fullStr Changes in white adipose tissue metabolism induced by resveratrol in rats
title_full_unstemmed Changes in white adipose tissue metabolism induced by resveratrol in rats
title_sort Changes in white adipose tissue metabolism induced by resveratrol in rats
dc.creator.none.fl_str_mv Alberdi, Goiuri
Rodríguez Rivera, Víctor Manuel
Miranda Gómez, Jonatan
Macarulla Arenaza, María Teresa
Arias Rueda, Noemí
Andrés Lacueva, Cristina
Portillo Baquedano, María Puy
author Alberdi, Goiuri
author_facet Alberdi, Goiuri
Rodríguez Rivera, Víctor Manuel
Miranda Gómez, Jonatan
Macarulla Arenaza, María Teresa
Arias Rueda, Noemí
Andrés Lacueva, Cristina
Portillo Baquedano, María Puy
author_role author
author2 Rodríguez Rivera, Víctor Manuel
Miranda Gómez, Jonatan
Macarulla Arenaza, María Teresa
Arias Rueda, Noemí
Andrés Lacueva, Cristina
Portillo Baquedano, María Puy
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv fat mobilization
linoleic acid
body fat
adipocytes
mice
diet
obesity
SIRT1
modulation
expression
ENDOCRINOLOGY AND METABOLISM
NUTRITION AND DIETETICS
MEDICINE
topic fat mobilization
linoleic acid
body fat
adipocytes
mice
diet
obesity
SIRT1
modulation
expression
ENDOCRINOLOGY AND METABOLISM
NUTRITION AND DIETETICS
MEDICINE
description Background: A remarkable range of biological functions have been ascribed to resveratrol. Recently, this polyphenol has been shown to have body fat lowering effects. The aim of the present study was to assess some of the potential underlying mechanisms of action which take place in adipose tissue. Methods: Sixteen male Sprague-Dawley rats were randomly divided into two groups: control and treated with 30 mg resveratrol/kg body weight/d. All rats were fed an obesogenic diet and after six weeks of treatment white adipose tissues were dissected. Lipoprotein lipase activity was assessed by fluorimetry, acetyl-CoA carboxylase by radiometry, and malic enzyme, glucose-6P-dehydrogenase and fatty acid synthase by spectrophotometry. Gene expression levels of acetyl-CoA carboxylase, fatty acid synthase, lipoprotein lipase, hormone-sensitive lipase, adipose triglyceride lipase, PPAR-gamma, SREBP-1c and perilipin were assessed by Real time RT-PCR. The amount of resveratrol metabolites in adipose tissue was measured by chromatography. Results: There was no difference in the final body weight of the rats; however, adipose tissues were significantly decreased in the resveratrol-treated group. Resveratrol reduced the activity of lipogenic enzymes, as well as that of heparin-releasable lipoprotein lipase. Moreover, a significant reduction was induced by this polyphenol in hormone-sensitive lipase mRNA levels. No significant changes were observed in other genes. Total amount of resveratrol metabolites in adipose tissue was 2.66 +/- 0.55 nmol/g tissue. Conclusions: It can be proposed that the body fat-lowering effect of resveratrol is mediated, at least in part, by a reduction in fatty acid uptake from circulating triacylglycerols and also in de novo lipogenesis.
publishDate 2011
dc.date.none.fl_str_mv 2011
2014
2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/11583
url http://hdl.handle.net/10810/11583
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MICINN/AGL2008-1005-ALI/
info:eu-repo/grantAgreement/MICINN/AGL2006-14228-C03-02/
http://www.nutritionandmetabolism.com/content/8/1/29
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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