Sequence-function relationships in phage-encoded bacterial cell wall lytic enzymes and their implications for phage-derived products design

23 p.-12 fig.-2 tab.

Detalles Bibliográficos
Autores: Vázquez, Roberto, García, Ernesto, García González, Pedro
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/239439
Acceso en línea:http://hdl.handle.net/10261/239439
Access Level:acceso abierto
Palabra clave:Endolysins
Bacteriophages
Bacteriophage therapy
Genomics
Bioinformatics
Antimicrobial agents
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spelling Sequence-function relationships in phage-encoded bacterial cell wall lytic enzymes and their implications for phage-derived products designRunning title: Bioinformatic analysis of phage lysin sequencesVázquez, RobertoGarcía, ErnestoGarcía González, PedroEndolysinsBacteriophagesBacteriophage therapyGenomicsBioinformaticsAntimicrobial agents23 p.-12 fig.-2 tab.Phage (endo)lysins are thought to be a viable alternative to usual antibiotic chemotherapy to fight resistant bacterial infections. However, a landscape view of lysins’ structure and properties regarding their function, with an applied focus, is somewhat lacking. Current literature suggests that specific features typical of lysins from phages infecting Gram-negative bacteria (G−) (higher net charge, amphipathic helices) are responsible for improved interaction with the G−envelope. Such antimicrobial peptide (AMP)-like elements are also of interest for antimicrobial molecules design. Thus, this study aims to provide an updated view on the primary structural landscape of phage lysins to clarify the evolutionary importance of several sequence-predicted properties, particularly for the interaction with the G−surface. A database of 2,182 lysin sequences was compiled, containing relevant information such as domain architectures, data on the phages’ host bacteria, and sequence -predicted physicochemical properties. Based on such classifiers, an investigation of the differential appearance of certain features was conducted. Such analyses revealed different lysin architectural variants that are preferably found in phages infecting certain bacterial hosts. Particularly, some physicochemical properties (higher net charge,hydrophobicity, hydrophobic moment, and aliphatic index) were associated with G−phage lysins, appearing specifically at their C-terminal end. Evidence on the remarkable genetic specialization of lysins regarding the features of the bacterial hosts has been provided, specifically supporting the nowadays common hypothesis that lysins from G− usually contain AMP-like regions.This study was funded by a grant from the Ministerio de Economía y Competitividad (MINECO-FEDER, SAF2017-88664-R). Additional funding was provided by the Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES), an initiative of the Instituto de Salud Carlos III.Peer reviewedAmerican Society for MicrobiologyMinisterio de Economía y Competitividad (España)Instituto de Salud Carlos IIIVázquez, Roberto [0000-0002-7919-552X]García, Ernesto [0000-0002-1741-5486]García, Pedro [0000-0001-6717-8717]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202120212021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/239439reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/SAF2017-88664-RVázquez, Roberto; García, Ernesto; García, Pedro; 2020; "Curated phage lysins collection including identifiers, amino acid sequences, functional domain predictions, architectures and physicochemical properties calculations "; DIGITAL.CSIC; http://dx.doi.org/10.20350/digitalCSIC/12674. http://hdl.handle.net/10261/221469http://doi.org/10.1128/JVI.00321-21Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2394392026-05-22T06:33:51Z
dc.title.none.fl_str_mv Sequence-function relationships in phage-encoded bacterial cell wall lytic enzymes and their implications for phage-derived products design
Running title: Bioinformatic analysis of phage lysin sequences
title Sequence-function relationships in phage-encoded bacterial cell wall lytic enzymes and their implications for phage-derived products design
spellingShingle Sequence-function relationships in phage-encoded bacterial cell wall lytic enzymes and their implications for phage-derived products design
Vázquez, Roberto
Endolysins
Bacteriophages
Bacteriophage therapy
Genomics
Bioinformatics
Antimicrobial agents
title_short Sequence-function relationships in phage-encoded bacterial cell wall lytic enzymes and their implications for phage-derived products design
title_full Sequence-function relationships in phage-encoded bacterial cell wall lytic enzymes and their implications for phage-derived products design
title_fullStr Sequence-function relationships in phage-encoded bacterial cell wall lytic enzymes and their implications for phage-derived products design
title_full_unstemmed Sequence-function relationships in phage-encoded bacterial cell wall lytic enzymes and their implications for phage-derived products design
title_sort Sequence-function relationships in phage-encoded bacterial cell wall lytic enzymes and their implications for phage-derived products design
dc.creator.none.fl_str_mv Vázquez, Roberto
García, Ernesto
García González, Pedro
author Vázquez, Roberto
author_facet Vázquez, Roberto
García, Ernesto
García González, Pedro
author_role author
author2 García, Ernesto
García González, Pedro
author2_role author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Instituto de Salud Carlos III
Vázquez, Roberto [0000-0002-7919-552X]
García, Ernesto [0000-0002-1741-5486]
García, Pedro [0000-0001-6717-8717]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Endolysins
Bacteriophages
Bacteriophage therapy
Genomics
Bioinformatics
Antimicrobial agents
topic Endolysins
Bacteriophages
Bacteriophage therapy
Genomics
Bioinformatics
Antimicrobial agents
description 23 p.-12 fig.-2 tab.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/239439
url http://hdl.handle.net/10261/239439
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/SAF2017-88664-R
Vázquez, Roberto; García, Ernesto; García, Pedro; 2020; "Curated phage lysins collection including identifiers, amino acid sequences, functional domain predictions, architectures and physicochemical properties calculations "; DIGITAL.CSIC; http://dx.doi.org/10.20350/digitalCSIC/12674. http://hdl.handle.net/10261/221469
http://doi.org/10.1128/JVI.00321-21
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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