Infection of Nicotiana benthamiana Plants with Potato Virus X (PVX)

12 p.-8 fig.

Detalles Bibliográficos
Autores: Aguilar, Emmanuel, Del Toro, Francisco, Chung, Bong-Nam, Canto, Tomás, Tenllado, Francisco
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/143208
Acceso en línea:http://hdl.handle.net/10261/143208
Access Level:acceso abierto
Palabra clave:Potato Virus X
Inoculation
Agroinfiltration
Heterologous protein expression
Virus-induced gene silencing
Gene function
id ES_39ae1bafc3f8bc2ddccbb6eff6548399
oai_identifier_str oai:digital.csic.es:10261/143208
network_acronym_str ES
network_name_str España
repository_id_str
spelling Infection of Nicotiana benthamiana Plants with Potato Virus X (PVX)Aguilar, EmmanuelDel Toro, FranciscoChung, Bong-NamCanto, TomásTenllado, FranciscoPotato Virus XInoculationAgroinfiltrationHeterologous protein expressionVirus-induced gene silencingGene function12 p.-8 fig.Potato Virus X (PVX) is the type member of Potexvirus genus, a group of plant viruses with a positive-strand RNA genome (~6.4 kb). PVX is able to establish compatible infections in Nicotiana benthamiana, a commonly used host in plant virology, leading to mild symptoms, such as chlorotic mosaic and mottling. PVX has been widely used as a viral vector for more than two decades (Chapman et al., 1992; Baulcombe et al., 1995; Aguilar et al., 2015). It provides a feasible means for the systemic expression in plants of heterologous proteins, such as avirulence factors, proteins with pharmacological properties, etc., (Hammond-Kosack et al., 1995; Gleba et al., 2014), and also as a tool to help decipher the function of genes in plants by virus-induced gene silencing (VIGS) (Lacomme and Chapman, 2008). Two different protocols, i.e., rubbing (A) and agroinfiltration (B), that allow efficient multiplication and propagation of PVX in N. benthamiana are described here in detail. The rubbing method requires previous infected sap, and infection is achieved by inducing mechanical damages to leaf tissue, allowing viral particles to penetrate the plant surface. Agroinfiltration needs previously modified agrobacterium to carry and deliver T-DNA with PVX sequences into the plant cell. Agrobacterium is grown until saturation and infection is established by infiltrating it into plant tissue with a syringe. Any of these two methods can be successfully applied, and the choice should be based mainly on the availability of material and time, but it is recommended to use agroinfiltration when chimeric viruses are being used.This work was supported by the Spanish Ministry of Economy and Competitiveness (Grant Ref. BIO2013-47940-R); The Rural Development Administration (RDA) of the Republic of Korea (Grant Refs. PJ00946102; PJ010246).Peer reviewedBio-protocol LLCMinisterio de Economía y Competitividad (España)Rural Development Administration (South Korea)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201720172016info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/143208reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.21769/BioProtoc.2063Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1432082026-05-22T06:33:51Z
dc.title.none.fl_str_mv Infection of Nicotiana benthamiana Plants with Potato Virus X (PVX)
title Infection of Nicotiana benthamiana Plants with Potato Virus X (PVX)
spellingShingle Infection of Nicotiana benthamiana Plants with Potato Virus X (PVX)
Aguilar, Emmanuel
Potato Virus X
Inoculation
Agroinfiltration
Heterologous protein expression
Virus-induced gene silencing
Gene function
title_short Infection of Nicotiana benthamiana Plants with Potato Virus X (PVX)
title_full Infection of Nicotiana benthamiana Plants with Potato Virus X (PVX)
title_fullStr Infection of Nicotiana benthamiana Plants with Potato Virus X (PVX)
title_full_unstemmed Infection of Nicotiana benthamiana Plants with Potato Virus X (PVX)
title_sort Infection of Nicotiana benthamiana Plants with Potato Virus X (PVX)
dc.creator.none.fl_str_mv Aguilar, Emmanuel
Del Toro, Francisco
Chung, Bong-Nam
Canto, Tomás
Tenllado, Francisco
author Aguilar, Emmanuel
author_facet Aguilar, Emmanuel
Del Toro, Francisco
Chung, Bong-Nam
Canto, Tomás
Tenllado, Francisco
author_role author
author2 Del Toro, Francisco
Chung, Bong-Nam
Canto, Tomás
Tenllado, Francisco
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Rural Development Administration (South Korea)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Potato Virus X
Inoculation
Agroinfiltration
Heterologous protein expression
Virus-induced gene silencing
Gene function
topic Potato Virus X
Inoculation
Agroinfiltration
Heterologous protein expression
Virus-induced gene silencing
Gene function
description 12 p.-8 fig.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/143208
url http://hdl.handle.net/10261/143208
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.21769/BioProtoc.2063
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Bio-protocol LLC
publisher.none.fl_str_mv Bio-protocol LLC
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869406185773334528
score 15,811543

Similares en LA Referencia