Ethanolysis of degummed soybean oil using magnetic CLEAs from Eversa® Transform

Eversa@ Transform magnetic crosslinked enzyme aggregates (Eversa-mCLEA) have been used to produce fatty acid ethyl esters (FAEEs) through the ethanolysis of soybean oil. Some variables influencing this reaction were studied using an experimental statistical design. After 12 hours of reaction, a maxi...

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Autores: Miranda, Letícia Passos, Guimarães, José R., Fernández-Lafuente, Roberto, Tardioli, Paulo W.
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/402692
Acesso em linha:http://hdl.handle.net/10261/402692
https://api.elsevier.com/content/abstract/scopus_id/105000669584
Access Level:acceso abierto
Palavra-chave:Biodiesel
Ethanolysis
Eversa
Lipases
Magnetic CLEAs
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spelling Ethanolysis of degummed soybean oil using magnetic CLEAs from Eversa® TransformMiranda, Letícia PassosGuimarães, José R.Fernández-Lafuente, RobertoTardioli, Paulo W.BiodieselEthanolysisEversaLipasesMagnetic CLEAsEversa@ Transform magnetic crosslinked enzyme aggregates (Eversa-mCLEA) have been used to produce fatty acid ethyl esters (FAEEs) through the ethanolysis of soybean oil. Some variables influencing this reaction were studied using an experimental statistical design. After 12 hours of reaction, a maximum FAEEs yield of 64 wt% was obtained using 4 Uest/g oil of Eversa-mCLEA, an anhydrous ethanol/refined oil molar ratio of 11, and a temperature of 40°C. Degummed oil and hydrated ethanol were used as more cost-effective alternatives, leading to an increase in FAEEs yield (up to 73 wt%). The initial reaction rate increased with a lower molar ratio of hydrated ethanol/degummed oil; however, the final yield remained similar. The combined use of Eversa-mCLEA and Lipozyme 435 resulted in 86 wt% FAEEs and 4 wt% of free fatty acids (FFAs) after 24 hours. A caustic polishing step of the product yielded 90 wt% FAEEs and 0.17 wt% FFAs. These findings show that, using these substrates, a more effective purification step (such as fractional distillation) is required for the product to meet international standards for biodiesel commercialization.We gratefully acknowledge the financial support from Ministerio de Ciencia e Innovación and Agencia Estatal de Investigación (Spanish Government) (PID2022–136535OB-I00), São Paulo Research Foundation (FAPESP, grant number 2016/10636–8), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior–Brasil (CAPES, Finance Code 001), and the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, project numbers 405889/2016–0).Peer reviewedElsevier BVAgencia Estatal de Investigación (España)Ministerio de Ciencia e Innovación (España)Sao Paulo Research FoundationCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil)Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil)Miranda, Letícia Passo [0000-0001-7006-9159]Guimarães, José R. [0000-0001-7559-2782]Fernández-Lafuente, Roberto [0000-0003-4976-7096]Tardioli, Paulo W. [0000-0002-5011-9881]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/402692https://api.elsevier.com/content/abstract/scopus_id/105000669584reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1016/j.jbiotec.2025.03.010Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4026922026-05-22T06:33:51Z
dc.title.none.fl_str_mv Ethanolysis of degummed soybean oil using magnetic CLEAs from Eversa® Transform
title Ethanolysis of degummed soybean oil using magnetic CLEAs from Eversa® Transform
spellingShingle Ethanolysis of degummed soybean oil using magnetic CLEAs from Eversa® Transform
Miranda, Letícia Passos
Biodiesel
Ethanolysis
Eversa
Lipases
Magnetic CLEAs
title_short Ethanolysis of degummed soybean oil using magnetic CLEAs from Eversa® Transform
title_full Ethanolysis of degummed soybean oil using magnetic CLEAs from Eversa® Transform
title_fullStr Ethanolysis of degummed soybean oil using magnetic CLEAs from Eversa® Transform
title_full_unstemmed Ethanolysis of degummed soybean oil using magnetic CLEAs from Eversa® Transform
title_sort Ethanolysis of degummed soybean oil using magnetic CLEAs from Eversa® Transform
dc.creator.none.fl_str_mv Miranda, Letícia Passos
Guimarães, José R.
Fernández-Lafuente, Roberto
Tardioli, Paulo W.
author Miranda, Letícia Passos
author_facet Miranda, Letícia Passos
Guimarães, José R.
Fernández-Lafuente, Roberto
Tardioli, Paulo W.
author_role author
author2 Guimarães, José R.
Fernández-Lafuente, Roberto
Tardioli, Paulo W.
author2_role author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
Ministerio de Ciencia e Innovación (España)
Sao Paulo Research Foundation
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil)
Miranda, Letícia Passo [0000-0001-7006-9159]
Guimarães, José R. [0000-0001-7559-2782]
Fernández-Lafuente, Roberto [0000-0003-4976-7096]
Tardioli, Paulo W. [0000-0002-5011-9881]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Biodiesel
Ethanolysis
Eversa
Lipases
Magnetic CLEAs
topic Biodiesel
Ethanolysis
Eversa
Lipases
Magnetic CLEAs
description Eversa@ Transform magnetic crosslinked enzyme aggregates (Eversa-mCLEA) have been used to produce fatty acid ethyl esters (FAEEs) through the ethanolysis of soybean oil. Some variables influencing this reaction were studied using an experimental statistical design. After 12 hours of reaction, a maximum FAEEs yield of 64 wt% was obtained using 4 Uest/g oil of Eversa-mCLEA, an anhydrous ethanol/refined oil molar ratio of 11, and a temperature of 40°C. Degummed oil and hydrated ethanol were used as more cost-effective alternatives, leading to an increase in FAEEs yield (up to 73 wt%). The initial reaction rate increased with a lower molar ratio of hydrated ethanol/degummed oil; however, the final yield remained similar. The combined use of Eversa-mCLEA and Lipozyme 435 resulted in 86 wt% FAEEs and 4 wt% of free fatty acids (FFAs) after 24 hours. A caustic polishing step of the product yielded 90 wt% FAEEs and 0.17 wt% FFAs. These findings show that, using these substrates, a more effective purification step (such as fractional distillation) is required for the product to meet international standards for biodiesel commercialization.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/402692
https://api.elsevier.com/content/abstract/scopus_id/105000669584
url http://hdl.handle.net/10261/402692
https://api.elsevier.com/content/abstract/scopus_id/105000669584
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1016/j.jbiotec.2025.03.010
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier BV
publisher.none.fl_str_mv Elsevier BV
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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