Staphylococcal pathogenicity island interference with helper phage reproduction is a paradigm of molecular parasitism

Staphylococcal pathogenicity islands (SaPIs) carry superantigen and resistance genes and are extremely widespread in Staphylococcus aureus and in other Gram-positive bacteria. SaPIs represent a major source of intrageneric horizontal gene transfer and a stealth conduit for intergeneric gene transfer...

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Autores: Ram, G, Chen, J, Kumar, K, Ross, HF, Ubeda, C, Damle, PK, Lane, KD, Penades, JR, Christie, GE, Novick, RP
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2012
País:España
Institución:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
Repositorio:r-FISABIO. Repositorio Institucional de Producción Científica
OAI Identifier:oai:fisabio.fundanetsuite.com:p14990
Acceso en línea:https://fisabio.portalinvestigacion.com/publicaciones/14990
Access Level:acceso abierto
Palabra clave:temperate phage
small terminase
capsid morphogenesis
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spelling Staphylococcal pathogenicity island interference with helper phage reproduction is a paradigm of molecular parasitismRam, GChen, JKumar, KRoss, HFUbeda, CDamle, PKLane, KDPenades, JRChristie, GENovick, RPtemperate phagesmall terminasecapsid morphogenesisStaphylococcal pathogenicity islands (SaPIs) carry superantigen and resistance genes and are extremely widespread in Staphylococcus aureus and in other Gram-positive bacteria. SaPIs represent a major source of intrageneric horizontal gene transfer and a stealth conduit for intergeneric gene transfer; they are phage satellites that exploit the life cycle of their temperate helper phages with elegant precision to enable their rapid replication and promiscuous spread. SaPIs also interfere with helper phage reproduction, blocking plaque formation, sharply reducing burst size and enhancing the survival of host cells following phage infection. Here, we show that SaPIs use several different strategies for phage interference, presumably the result of convergent evolution. One strategy, not described previously in the bacteriophage microcosm, involves a SaPI-encoded protein that directly and specifically interferes with phage DNA packaging by blocking the phage terminase small subunit. Another strategy involves interference with phage reproduction by diversion of the vast majority of virion proteins to the formation of SaPI-specific small infectious particles. Several SaPIs use both of these strategies, and at least one uses neither but possesses a third. Our studies illuminate a key feature of the evolutionary strategy of these mobile genetic elements, in addition to their carriage of important genes-interference with helper phage reproduction, which could ensure their transferability and long-term persistence.National Academy of Sciences2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://fisabio.portalinvestigacion.com/publicaciones/14990PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICAISSN: 10916490ISSNe: 00278424reponame:r-FISABIO. Repositorio Institucional de Producción Científicainstname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)Inglésinfo:eu-repo/semantics/openAccessoai:fisabio.fundanetsuite.com:p149902026-06-11T12:45:17Z
dc.title.none.fl_str_mv Staphylococcal pathogenicity island interference with helper phage reproduction is a paradigm of molecular parasitism
title Staphylococcal pathogenicity island interference with helper phage reproduction is a paradigm of molecular parasitism
spellingShingle Staphylococcal pathogenicity island interference with helper phage reproduction is a paradigm of molecular parasitism
Ram, G
temperate phage
small terminase
capsid morphogenesis
title_short Staphylococcal pathogenicity island interference with helper phage reproduction is a paradigm of molecular parasitism
title_full Staphylococcal pathogenicity island interference with helper phage reproduction is a paradigm of molecular parasitism
title_fullStr Staphylococcal pathogenicity island interference with helper phage reproduction is a paradigm of molecular parasitism
title_full_unstemmed Staphylococcal pathogenicity island interference with helper phage reproduction is a paradigm of molecular parasitism
title_sort Staphylococcal pathogenicity island interference with helper phage reproduction is a paradigm of molecular parasitism
dc.creator.none.fl_str_mv Ram, G
Chen, J
Kumar, K
Ross, HF
Ubeda, C
Damle, PK
Lane, KD
Penades, JR
Christie, GE
Novick, RP
author Ram, G
author_facet Ram, G
Chen, J
Kumar, K
Ross, HF
Ubeda, C
Damle, PK
Lane, KD
Penades, JR
Christie, GE
Novick, RP
author_role author
author2 Chen, J
Kumar, K
Ross, HF
Ubeda, C
Damle, PK
Lane, KD
Penades, JR
Christie, GE
Novick, RP
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv temperate phage
small terminase
capsid morphogenesis
topic temperate phage
small terminase
capsid morphogenesis
description Staphylococcal pathogenicity islands (SaPIs) carry superantigen and resistance genes and are extremely widespread in Staphylococcus aureus and in other Gram-positive bacteria. SaPIs represent a major source of intrageneric horizontal gene transfer and a stealth conduit for intergeneric gene transfer; they are phage satellites that exploit the life cycle of their temperate helper phages with elegant precision to enable their rapid replication and promiscuous spread. SaPIs also interfere with helper phage reproduction, blocking plaque formation, sharply reducing burst size and enhancing the survival of host cells following phage infection. Here, we show that SaPIs use several different strategies for phage interference, presumably the result of convergent evolution. One strategy, not described previously in the bacteriophage microcosm, involves a SaPI-encoded protein that directly and specifically interferes with phage DNA packaging by blocking the phage terminase small subunit. Another strategy involves interference with phage reproduction by diversion of the vast majority of virion proteins to the formation of SaPI-specific small infectious particles. Several SaPIs use both of these strategies, and at least one uses neither but possesses a third. Our studies illuminate a key feature of the evolutionary strategy of these mobile genetic elements, in addition to their carriage of important genes-interference with helper phage reproduction, which could ensure their transferability and long-term persistence.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://fisabio.portalinvestigacion.com/publicaciones/14990
url https://fisabio.portalinvestigacion.com/publicaciones/14990
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv National Academy of Sciences
publisher.none.fl_str_mv National Academy of Sciences
dc.source.none.fl_str_mv PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN: 10916490
ISSNe: 00278424
reponame:r-FISABIO. Repositorio Institucional de Producción Científica
instname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
instname_str Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
reponame_str r-FISABIO. Repositorio Institucional de Producción Científica
collection r-FISABIO. Repositorio Institucional de Producción Científica
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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