Spatial and Temporal Variability of Microbial Nitrogen Cycling Genes in Arctic Streams
The Arctic is undergoing rapid climate change, with thawing permafrost and shifts in vegetation altering nitrogen (N) delivery into streams. These changes can significantly affect microbial biofilm diversity and functional roles, yet knowledge of streambed microbial biofilms remains scarce across th...
| Autores: | , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:dnet:recercat____::5517f7ecfb155f16688ee71bc72bcff4 |
| Acceso en línea: | http://hdl.handle.net/10256/28664 https://hdl.handle.net/10256/28664 |
| Access Level: | acceso abierto |
| Palabra clave: | Microbial communities Prokaryotic Arctic regions Nitrogen Canvi climàtic Permafrost Biodiversitat microbiana |
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Spatial and Temporal Variability of Microbial Nitrogen Cycling Genes in Arctic StreamsHolmboe, Cecilie M.H.Riis, TennaHan, X.Romaní i Cornet, Anna M.Kjær, J. B.Tank, JenniferGimenez Grau, PauHille, E.D'Acqui, Luigi P.Catalán, NúriaPastor, OliverasMicrobial communitiesProkaryoticArctic regionsNitrogenCanvi climàticPermafrostBiodiversitat microbianaThe Arctic is undergoing rapid climate change, with thawing permafrost and shifts in vegetation altering nitrogen (N) delivery into streams. These changes can significantly affect microbial biofilm diversity and functional roles, yet knowledge of streambed microbial biofilms remains scarce across the Arctic. This study examines the biogeographic and temporal patterns of prokaryotic sediment diversity and N functional genes across a biogeographical gradient in Arctic regions. We sampled sediment from 27 streams across four Arctic regions, including 14 streams in Greenland that were sampled at three timepoints throughout the open‐water; all other streams were sampled once in midsummer. We analyzed 16S rRNA gene sequencing and quantified six genes involved in the N‐fixation (nifH), nitrification (amoA, nxrB), and denitrification (nirS, norB and nosZ). Our results showed that prokaryotic and N functional gene abundances varied among regions, with higher abundance in areas with more catchment vegetation and higher organic matter availability. However, the composition of prokaryote communities and N functional genes showed no regional differences. Prokaryotic abundance and diversity tended to increase toward late summer. This study highlights how catchment properties, particularly organic matter and vegetation, influence stream prokaryotic communities and their role in N cycling, providing key insights into ecosystem responses to climate change in the ArcticOpen Access funding provided thanks to the CRUE-CSIC agreement with Wiley. This work was funded by H2020 through INTERACT Transnational Access (Grant Agreement No. 730938), Villum Experiment funds (ref 00028351), and Aarhus University Research Foundation (grant to TR). NC received funding from the European Union's Horizon 2020 research and innovation program under the Marie Sklodowska‐Curie grant agreement no. 839709. AP is currently supported by a “Ramón y Cajal” Fellowship (RYC2022‐036661‐I) from the Spanish Ministry of Science, Innovation and Universities. AMR acknowledges the funding from the AGAUR‐ICREA Academia award. We gratefully acknowledge the contributions of the Danish National Research Foundation (DNRF 185) to the Center for Ice‐Free Arctic Research (CIFAR), Aarhus University.13Scopus2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer-reviewedapplication/pdfhttp://hdl.handle.net/10256/28664https://hdl.handle.net/10256/28664Global Biogeochemical Cycles, 2026, 40Articles publicats (IEA)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/issn/0886-6236info:eu-repo/semantics/altIdentifier/eissn/1944-9224Attribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:dnet:recercat____::5517f7ecfb155f16688ee71bc72bcff42026-05-29T05:05:01Z |
| dc.title.none.fl_str_mv |
Spatial and Temporal Variability of Microbial Nitrogen Cycling Genes in Arctic Streams |
| title |
Spatial and Temporal Variability of Microbial Nitrogen Cycling Genes in Arctic Streams |
| spellingShingle |
Spatial and Temporal Variability of Microbial Nitrogen Cycling Genes in Arctic Streams Holmboe, Cecilie M.H. Microbial communities Prokaryotic Arctic regions Nitrogen Canvi climàtic Permafrost Biodiversitat microbiana |
| title_short |
Spatial and Temporal Variability of Microbial Nitrogen Cycling Genes in Arctic Streams |
| title_full |
Spatial and Temporal Variability of Microbial Nitrogen Cycling Genes in Arctic Streams |
| title_fullStr |
Spatial and Temporal Variability of Microbial Nitrogen Cycling Genes in Arctic Streams |
| title_full_unstemmed |
Spatial and Temporal Variability of Microbial Nitrogen Cycling Genes in Arctic Streams |
| title_sort |
Spatial and Temporal Variability of Microbial Nitrogen Cycling Genes in Arctic Streams |
| dc.creator.none.fl_str_mv |
Holmboe, Cecilie M.H. Riis, Tenna Han, X. Romaní i Cornet, Anna M. Kjær, J. B. Tank, Jennifer Gimenez Grau, Pau Hille, E. D'Acqui, Luigi P. Catalán, Núria Pastor, Oliveras |
| author |
Holmboe, Cecilie M.H. |
| author_facet |
Holmboe, Cecilie M.H. Riis, Tenna Han, X. Romaní i Cornet, Anna M. Kjær, J. B. Tank, Jennifer Gimenez Grau, Pau Hille, E. D'Acqui, Luigi P. Catalán, Núria Pastor, Oliveras |
| author_role |
author |
| author2 |
Riis, Tenna Han, X. Romaní i Cornet, Anna M. Kjær, J. B. Tank, Jennifer Gimenez Grau, Pau Hille, E. D'Acqui, Luigi P. Catalán, Núria Pastor, Oliveras |
| author2_role |
author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Microbial communities Prokaryotic Arctic regions Nitrogen Canvi climàtic Permafrost Biodiversitat microbiana |
| topic |
Microbial communities Prokaryotic Arctic regions Nitrogen Canvi climàtic Permafrost Biodiversitat microbiana |
| description |
The Arctic is undergoing rapid climate change, with thawing permafrost and shifts in vegetation altering nitrogen (N) delivery into streams. These changes can significantly affect microbial biofilm diversity and functional roles, yet knowledge of streambed microbial biofilms remains scarce across the Arctic. This study examines the biogeographic and temporal patterns of prokaryotic sediment diversity and N functional genes across a biogeographical gradient in Arctic regions. We sampled sediment from 27 streams across four Arctic regions, including 14 streams in Greenland that were sampled at three timepoints throughout the open‐water; all other streams were sampled once in midsummer. We analyzed 16S rRNA gene sequencing and quantified six genes involved in the N‐fixation (nifH), nitrification (amoA, nxrB), and denitrification (nirS, norB and nosZ). Our results showed that prokaryotic and N functional gene abundances varied among regions, with higher abundance in areas with more catchment vegetation and higher organic matter availability. However, the composition of prokaryote communities and N functional genes showed no regional differences. Prokaryotic abundance and diversity tended to increase toward late summer. This study highlights how catchment properties, particularly organic matter and vegetation, influence stream prokaryotic communities and their role in N cycling, providing key insights into ecosystem responses to climate change in the Arctic |
| publishDate |
2025 |
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2025 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion peer-reviewed |
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article |
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publishedVersion |
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http://hdl.handle.net/10256/28664 https://hdl.handle.net/10256/28664 |
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http://hdl.handle.net/10256/28664 https://hdl.handle.net/10256/28664 |
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Inglés |
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Inglés |
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info:eu-repo/semantics/altIdentifier/issn/0886-6236 info:eu-repo/semantics/altIdentifier/eissn/1944-9224 |
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Attribution-NonCommercial 4.0 International http://creativecommons.org/licenses/by-nc/4.0/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial 4.0 International http://creativecommons.org/licenses/by-nc/4.0/ |
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openAccess |
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application/pdf |
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Scopus |
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Scopus |
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Global Biogeochemical Cycles, 2026, 40 Articles publicats (IEA) reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
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