Pediococcus acidilactici CECT9879 (pA1c) counteracts the effect of a high-glucose exposure in c. elegans by affecting the insulin signaling pathway (IIS)

Yavorov-Dayliev, D. (Deyan)|||/items/35f99656-3058-4d65-88bd-d42c6e50df98; Milagro-Yoldi, F.I. (Fermín Ignacio)|||/items/07cf7af6-1f5f-4720-8c14-5197a7a724eb; Ayo, J. (Josune)|||/items/a7001b37-cdcc-436a-b894-14ef479e92c5; Oneca, M. (María)|||/items/e65ba0f4-287c-4bcc-8933-723d0f1679a1; Aranaz-Oroz, P. (Paula)|||/items/8f6060d1-d2d4-425b-ac46-ffe00907395a

Pediococcus acidilactici CECT9879 (pA1c) counteracts the effect of a high-glucose exposure in c. elegans by affecting the insulin signaling pathway (IIS)

The increasing prevalence of metabolic syndrome-related diseases, including type-2 diabetes and obesity, makes it urgent to develop new alternative therapies, such as probiotics. In this study, we have used Caenorhabditis elegans under a high-glucose condition as a model to examine the potential pro...

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Detalles Bibliográficos
Autores:	Yavorov-Dayliev, D. (Deyan)\|\|\|/items/35f99656-3058-4d65-88bd-d42c6e50df98, Milagro-Yoldi, F.I. (Fermín Ignacio)\|\|\|/items/07cf7af6-1f5f-4720-8c14-5197a7a724eb, Ayo, J. (Josune)\|\|\|/items/a7001b37-cdcc-436a-b894-14ef479e92c5, Oneca, M. (María)\|\|\|/items/e65ba0f4-287c-4bcc-8933-723d0f1679a1, Aranaz-Oroz, P. (Paula)\|\|\|/items/8f6060d1-d2d4-425b-ac46-ffe00907395a
Tipo de recurso:	artículo
Fecha de publicación:	2022
País:	España
Institución:	Universidad de Navarra
Repositorio:	Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:	inglés
OAI Identifier:	oai:dadun.unav.edu:10171/116690
Acceso en línea:	https://hdl.handle.net/10171/116690
Access Level:	acceso abierto
Palabra clave:	Caenorhabditis elegans Daf-16 Diabetes Insulin-signaling-pathway Obesity Probiotic β-oxidation.

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spelling	Pediococcus acidilactici CECT9879 (pA1c) counteracts the effect of a high-glucose exposure in c. elegans by affecting the insulin signaling pathway (IIS)Yavorov-Dayliev, D. (Deyan)\|\|\|/items/35f99656-3058-4d65-88bd-d42c6e50df98Milagro-Yoldi, F.I. (Fermín Ignacio)\|\|\|/items/07cf7af6-1f5f-4720-8c14-5197a7a724ebAyo, J. (Josune)\|\|\|/items/a7001b37-cdcc-436a-b894-14ef479e92c5Oneca, M. (María)\|\|\|/items/e65ba0f4-287c-4bcc-8933-723d0f1679a1Aranaz-Oroz, P. (Paula)\|\|\|/items/8f6060d1-d2d4-425b-ac46-ffe00907395aCaenorhabditis elegansDaf-16DiabetesInsulin-signaling-pathwayObesityProbioticβ-oxidation.The increasing prevalence of metabolic syndrome-related diseases, including type-2 diabetes and obesity, makes it urgent to develop new alternative therapies, such as probiotics. In this study, we have used Caenorhabditis elegans under a high-glucose condition as a model to examine the potential probiotic activities of Pediococcusacidilactici CECT9879 (pA1c). The supplementation with pA1c reduced C. elegans fat accumulation in a nematode growth medium (NGM) and in a high-glucose (10 mM) NGM medium. Moreover, treatment with pA1c counteracted the effect of the high glucose by reducing reactive oxygen species by 20%, retarding the aging process and extending the nematode median survival (>2 days in comparison with untreated control worms). Gene expression analyses demonstrated that the probiotic metabolic syndrome-alleviating activities were mediated by modulation of the insulin/IGF-1 signaling pathway (IIS) through the reversion of the glucose-nuclear-localization of daf-16 and the overexpression of ins-6 and daf-16 mediators, increased expression of fatty acid (FA) peroxisomal β-oxidation genes, and downregulation of FA biosynthesis key genes. Taken together, our data suggest that pA1c could be considered a potential probiotic strain for the prevention of the metabolic syndrome-related disturbances and highlight the use of C. elegans as an appropriate in vivo model for the study of the mechanisms underlying these diseases.MDPIDadun. Depósito Académico Digital Universidad de Navarra20222022-01-0120222022-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/116690reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/1166902026-06-21T12:47:57Z
dc.title.none.fl_str_mv	Pediococcus acidilactici CECT9879 (pA1c) counteracts the effect of a high-glucose exposure in c. elegans by affecting the insulin signaling pathway (IIS)
title	Pediococcus acidilactici CECT9879 (pA1c) counteracts the effect of a high-glucose exposure in c. elegans by affecting the insulin signaling pathway (IIS)
spellingShingle	Pediococcus acidilactici CECT9879 (pA1c) counteracts the effect of a high-glucose exposure in c. elegans by affecting the insulin signaling pathway (IIS) Yavorov-Dayliev, D. (Deyan)\|\|\|/items/35f99656-3058-4d65-88bd-d42c6e50df98 Caenorhabditis elegans Daf-16 Diabetes Insulin-signaling-pathway Obesity Probiotic β-oxidation.
title_short	Pediococcus acidilactici CECT9879 (pA1c) counteracts the effect of a high-glucose exposure in c. elegans by affecting the insulin signaling pathway (IIS)
title_full	Pediococcus acidilactici CECT9879 (pA1c) counteracts the effect of a high-glucose exposure in c. elegans by affecting the insulin signaling pathway (IIS)
title_fullStr	Pediococcus acidilactici CECT9879 (pA1c) counteracts the effect of a high-glucose exposure in c. elegans by affecting the insulin signaling pathway (IIS)
title_full_unstemmed	Pediococcus acidilactici CECT9879 (pA1c) counteracts the effect of a high-glucose exposure in c. elegans by affecting the insulin signaling pathway (IIS)
title_sort	Pediococcus acidilactici CECT9879 (pA1c) counteracts the effect of a high-glucose exposure in c. elegans by affecting the insulin signaling pathway (IIS)
dc.creator.none.fl_str_mv	Yavorov-Dayliev, D. (Deyan)\|\|\|/items/35f99656-3058-4d65-88bd-d42c6e50df98 Milagro-Yoldi, F.I. (Fermín Ignacio)\|\|\|/items/07cf7af6-1f5f-4720-8c14-5197a7a724eb Ayo, J. (Josune)\|\|\|/items/a7001b37-cdcc-436a-b894-14ef479e92c5 Oneca, M. (María)\|\|\|/items/e65ba0f4-287c-4bcc-8933-723d0f1679a1 Aranaz-Oroz, P. (Paula)\|\|\|/items/8f6060d1-d2d4-425b-ac46-ffe00907395a
author	Yavorov-Dayliev, D. (Deyan)\|\|\|/items/35f99656-3058-4d65-88bd-d42c6e50df98
author_facet	Yavorov-Dayliev, D. (Deyan)\|\|\|/items/35f99656-3058-4d65-88bd-d42c6e50df98 Milagro-Yoldi, F.I. (Fermín Ignacio)\|\|\|/items/07cf7af6-1f5f-4720-8c14-5197a7a724eb Ayo, J. (Josune)\|\|\|/items/a7001b37-cdcc-436a-b894-14ef479e92c5 Oneca, M. (María)\|\|\|/items/e65ba0f4-287c-4bcc-8933-723d0f1679a1 Aranaz-Oroz, P. (Paula)\|\|\|/items/8f6060d1-d2d4-425b-ac46-ffe00907395a
author_role	author
author2	Milagro-Yoldi, F.I. (Fermín Ignacio)\|\|\|/items/07cf7af6-1f5f-4720-8c14-5197a7a724eb Ayo, J. (Josune)\|\|\|/items/a7001b37-cdcc-436a-b894-14ef479e92c5 Oneca, M. (María)\|\|\|/items/e65ba0f4-287c-4bcc-8933-723d0f1679a1 Aranaz-Oroz, P. (Paula)\|\|\|/items/8f6060d1-d2d4-425b-ac46-ffe00907395a
author2_role	author author author author
dc.contributor.none.fl_str_mv	Dadun. Depósito Académico Digital Universidad de Navarra
dc.subject.none.fl_str_mv	Caenorhabditis elegans Daf-16 Diabetes Insulin-signaling-pathway Obesity Probiotic β-oxidation.
topic	Caenorhabditis elegans Daf-16 Diabetes Insulin-signaling-pathway Obesity Probiotic β-oxidation.
description	The increasing prevalence of metabolic syndrome-related diseases, including type-2 diabetes and obesity, makes it urgent to develop new alternative therapies, such as probiotics. In this study, we have used Caenorhabditis elegans under a high-glucose condition as a model to examine the potential probiotic activities of Pediococcusacidilactici CECT9879 (pA1c). The supplementation with pA1c reduced C. elegans fat accumulation in a nematode growth medium (NGM) and in a high-glucose (10 mM) NGM medium. Moreover, treatment with pA1c counteracted the effect of the high glucose by reducing reactive oxygen species by 20%, retarding the aging process and extending the nematode median survival (>2 days in comparison with untreated control worms). Gene expression analyses demonstrated that the probiotic metabolic syndrome-alleviating activities were mediated by modulation of the insulin/IGF-1 signaling pathway (IIS) through the reversion of the glucose-nuclear-localization of daf-16 and the overexpression of ins-6 and daf-16 mediators, increased expression of fatty acid (FA) peroxisomal β-oxidation genes, and downregulation of FA biosynthesis key genes. Taken together, our data suggest that pA1c could be considered a potential probiotic strain for the prevention of the metabolic syndrome-related disturbances and highlight the use of C. elegans as an appropriate in vivo model for the study of the mechanisms underlying these diseases.
publishDate	2022
dc.date.none.fl_str_mv	2022 2022-01-01 2022 2022-01-01
dc.type.none.fl_str_mv	journal article http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	https://hdl.handle.net/10171/116690
url	https://hdl.handle.net/10171/116690
dc.language.none.fl_str_mv	Inglés eng
language_invalid_str_mv	Inglés
language	eng
dc.rights.none.fl_str_mv	open access http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv	info:eu-repo/semantics/openAccess
rights_invalid_str_mv	open access http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.publisher.none.fl_str_mv	MDPI
publisher.none.fl_str_mv	MDPI
dc.source.none.fl_str_mv	reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra instname:Universidad de Navarra
instname_str	Universidad de Navarra
reponame_str	Dadun. Depósito Académico Digital de la Universidad de Navarra
collection	Dadun. Depósito Académico Digital de la Universidad de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score	15,811543

Pediococcus acidilactici CECT9879 (pA1c) counteracts the effect of a high-glucose exposure in c. elegans by affecting the insulin signaling pathway (IIS)

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