Comparative analysis of wild-type accessions reveals novel determinants of Arabidopsis seed longevity

[EN] Understanding the genetic factors involved in seed longevity is of paramount importance in agricultural and ecological contexts. The polygenic nature of this trait suggests that many of them remain undiscovered. Here, we exploited the contrasting seed longevity found amongst Arabidopsis thalian...

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Autores: Niñoles Rodenes, Regina|||0000-0002-7862-9509, Planes Ferrer, Mª Dolores|||0000-0002-5347-8660, Renard, Joan|||0000-0003-1797-1578, Bueso Rodenas, Eduardo|||0000-0002-0828-121X, Serrano Salom, Ramón, Gadea Vacas, José|||0000-0002-3612-7914, Arjona, Paloma, Ruiz-Pastor, Carmen, Chazarra, Rubén, Forment, Javier, Kranner, Ilse, Roach, Thomas
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/197250
Acceso en línea:https://riunet.upv.es/handle/10251/197250
Access Level:acceso abierto
Palabra clave:Arabidopsis, Glutathione
Longevity
Natural variation
Seed
Transcriptomics
BIOQUIMICA Y BIOLOGIA MOLECULAR
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spelling Comparative analysis of wild-type accessions reveals novel determinants of Arabidopsis seed longevityNiñoles Rodenes, Regina|||0000-0002-7862-9509Planes Ferrer, Mª Dolores|||0000-0002-5347-8660Renard, Joan|||0000-0003-1797-1578Bueso Rodenas, Eduardo|||0000-0002-0828-121XSerrano Salom, RamónGadea Vacas, José|||0000-0002-3612-7914Arjona, PalomaRuiz-Pastor, CarmenChazarra, RubénForment, JavierKranner, IlseRoach, ThomasArabidopsis, GlutathioneLongevityNatural variationSeedTranscriptomicsBIOQUIMICA Y BIOLOGIA MOLECULAR[EN] Understanding the genetic factors involved in seed longevity is of paramount importance in agricultural and ecological contexts. The polygenic nature of this trait suggests that many of them remain undiscovered. Here, we exploited the contrasting seed longevity found amongst Arabidopsis thaliana accessions to further understand this phenomenon. Concentrations of glutathione were higher in longer-lived than shorter-lived accessions, supporting that redox poise plays a prominent role in seed longevity. However, high seed permeability, normally associated with shorter longevity, is also present in long-lived accessions. Dry seed transcriptome analysis indicated that the contribution to longevity of stored messenger RNA (mRNAs) is complex, including mainly accession-specific mechanisms. The detrimental effect on longevity caused by other factors may be counterbalanced by higher levels of specific mRNAs stored in dry seeds, for instance those of heat-shock proteins. Indeed, loss-of-function mutant analysis demonstrated that heat-shock factors HSF1A and 1B contributed to longevity. Furthermore, mutants of the stress-granule zinc-finger protein TZF9 or the spliceosome subunits MOS4 or MAC3A/MAC3B, extended seed longevity, positioning RNA as a novel player in the regulation of seed viability. mRNAs of proteins with putative relevance to longevity were also abundant in shorter-lived accessions, reinforcing the idea that resistance to ageing is determined by multiple factors.Spanish Ministerio de Economia Industria y Competitividad, action BIO2017-88898-PBlackwell PublishingInstituto Universitario Mixto de Biología Molecular y Celular de PlantasDepartamento de BiotecnologíaEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalAgencia Estatal de InvestigaciónUniversitat Politècnica de ValènciaRepositorio Institucional de la Universitat Politècnica de València Riunet20222022-09-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/197250reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 BIO2017-88898-P REDES REGULADORAS DE LA LONGEVIDAD DE SEMILLAS QUE ACTUAN A TRAVES DE LA PERMEABILIDAD DE LA CUBIERTA, DEFENSAS ANTIOXIDANTES Y ENZIMAS REPARADORASopen accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1972502026-06-13T07:49:27Z
dc.title.none.fl_str_mv Comparative analysis of wild-type accessions reveals novel determinants of Arabidopsis seed longevity
title Comparative analysis of wild-type accessions reveals novel determinants of Arabidopsis seed longevity
spellingShingle Comparative analysis of wild-type accessions reveals novel determinants of Arabidopsis seed longevity
Niñoles Rodenes, Regina|||0000-0002-7862-9509
Arabidopsis, Glutathione
Longevity
Natural variation
Seed
Transcriptomics
BIOQUIMICA Y BIOLOGIA MOLECULAR
title_short Comparative analysis of wild-type accessions reveals novel determinants of Arabidopsis seed longevity
title_full Comparative analysis of wild-type accessions reveals novel determinants of Arabidopsis seed longevity
title_fullStr Comparative analysis of wild-type accessions reveals novel determinants of Arabidopsis seed longevity
title_full_unstemmed Comparative analysis of wild-type accessions reveals novel determinants of Arabidopsis seed longevity
title_sort Comparative analysis of wild-type accessions reveals novel determinants of Arabidopsis seed longevity
dc.creator.none.fl_str_mv Niñoles Rodenes, Regina|||0000-0002-7862-9509
Planes Ferrer, Mª Dolores|||0000-0002-5347-8660
Renard, Joan|||0000-0003-1797-1578
Bueso Rodenas, Eduardo|||0000-0002-0828-121X
Serrano Salom, Ramón
Gadea Vacas, José|||0000-0002-3612-7914
Arjona, Paloma
Ruiz-Pastor, Carmen
Chazarra, Rubén
Forment, Javier
Kranner, Ilse
Roach, Thomas
author Niñoles Rodenes, Regina|||0000-0002-7862-9509
author_facet Niñoles Rodenes, Regina|||0000-0002-7862-9509
Planes Ferrer, Mª Dolores|||0000-0002-5347-8660
Renard, Joan|||0000-0003-1797-1578
Bueso Rodenas, Eduardo|||0000-0002-0828-121X
Serrano Salom, Ramón
Gadea Vacas, José|||0000-0002-3612-7914
Arjona, Paloma
Ruiz-Pastor, Carmen
Chazarra, Rubén
Forment, Javier
Kranner, Ilse
Roach, Thomas
author_role author
author2 Planes Ferrer, Mª Dolores|||0000-0002-5347-8660
Renard, Joan|||0000-0003-1797-1578
Bueso Rodenas, Eduardo|||0000-0002-0828-121X
Serrano Salom, Ramón
Gadea Vacas, José|||0000-0002-3612-7914
Arjona, Paloma
Ruiz-Pastor, Carmen
Chazarra, Rubén
Forment, Javier
Kranner, Ilse
Roach, Thomas
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto Universitario Mixto de Biología Molecular y Celular de Plantas
Departamento de Biotecnología
Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural
Agencia Estatal de Investigación
Universitat Politècnica de València
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Arabidopsis, Glutathione
Longevity
Natural variation
Seed
Transcriptomics
BIOQUIMICA Y BIOLOGIA MOLECULAR
topic Arabidopsis, Glutathione
Longevity
Natural variation
Seed
Transcriptomics
BIOQUIMICA Y BIOLOGIA MOLECULAR
description [EN] Understanding the genetic factors involved in seed longevity is of paramount importance in agricultural and ecological contexts. The polygenic nature of this trait suggests that many of them remain undiscovered. Here, we exploited the contrasting seed longevity found amongst Arabidopsis thaliana accessions to further understand this phenomenon. Concentrations of glutathione were higher in longer-lived than shorter-lived accessions, supporting that redox poise plays a prominent role in seed longevity. However, high seed permeability, normally associated with shorter longevity, is also present in long-lived accessions. Dry seed transcriptome analysis indicated that the contribution to longevity of stored messenger RNA (mRNAs) is complex, including mainly accession-specific mechanisms. The detrimental effect on longevity caused by other factors may be counterbalanced by higher levels of specific mRNAs stored in dry seeds, for instance those of heat-shock proteins. Indeed, loss-of-function mutant analysis demonstrated that heat-shock factors HSF1A and 1B contributed to longevity. Furthermore, mutants of the stress-granule zinc-finger protein TZF9 or the spliceosome subunits MOS4 or MAC3A/MAC3B, extended seed longevity, positioning RNA as a novel player in the regulation of seed viability. mRNAs of proteins with putative relevance to longevity were also abundant in shorter-lived accessions, reinforcing the idea that resistance to ageing is determined by multiple factors.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-09-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/197250
url https://riunet.upv.es/handle/10251/197250
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 BIO2017-88898-P REDES REGULADORAS DE LA LONGEVIDAD DE SEMILLAS QUE ACTUAN A TRAVES DE LA PERMEABILIDAD DE LA CUBIERTA, DEFENSAS ANTIOXIDANTES Y ENZIMAS REPARADORAS
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Blackwell Publishing
publisher.none.fl_str_mv Blackwell Publishing
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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