Addition of reduced glutathione (GSH) to freezing medium reduces intracellular ROS levels in donkey sperm

In donkeys, the use of frozen-thawed sperm for artificial insemination (AI) leads to low fertility rates. Furthermore, donkey sperm produce a large amount of reactive oxygen species (ROS), and post-AI inflammation induces the formation of neutrophil extracellular traps (NETosis), which further gener...

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Autores: Yáñez-Ortiz, Iván, Catalán, Jaime, Delgado Bermúdez, Ariadna, Carluccio, Augusto, Miró, Jordi, Yeste Oliveras, Marc
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/20260
Acceso en línea:http://hdl.handle.net/10256/20260
Access Level:acceso abierto
Palabra clave:Ases -- Espermatozoides
Donkeys -- Spermatozoa
Espermatozoides
Spermatozoa
Semen -- Crioconservació
Semen -- Cryopreservation
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oai_identifier_str oai:recercat.cat:10256/20260
network_acronym_str ES
network_name_str España
repository_id_str
spelling Addition of reduced glutathione (GSH) to freezing medium reduces intracellular ROS levels in donkey spermYáñez-Ortiz, IvánCatalán, JaimeDelgado Bermúdez, AriadnaCarluccio, AugustoMiró, JordiYeste Oliveras, MarcAses -- EspermatozoidesDonkeys -- SpermatozoaEspermatozoidesSpermatozoaSemen -- CrioconservacióSemen -- CryopreservationIn donkeys, the use of frozen-thawed sperm for artificial insemination (AI) leads to low fertility rates. Furthermore, donkey sperm produce a large amount of reactive oxygen species (ROS), and post-AI inflammation induces the formation of neutrophil extracellular traps (NETosis), which further generates many more ROS. These high ROS levels may induce lipid peroxidation in the sperm plasma membrane, thus affecting its integrity. Enzymatic and non-enzymatic antioxidants, mainly found in the seminal plasma (SP), are responsible for maintaining the redox balance. However, this fluid is removed prior to cryopreservation, thereby exposing sperm cells to further oxidative stress. The exogenous addition of antioxidants to the freezing medium can reduce the detrimental effects caused by ROS generation. Therefore, the aim of this study was to evaluate how the addition of different reduced glutathione (GSH) concentrations (control, 2 mM, 4 mM, 6 mM, 8 mM, and 10 mM) to fresh sperm affect their cryotolerance. Total and progressive motility, kinematic parameters and motile sperm subpopulations were significantly (p < 0.05) different from the control in treatments containing 8 mM and 10 mM GSH, but not at lower concentrations. Plasma and acrosome membrane integrity, mitochondrial membrane potential (MMP) and intracellular superoxide levels (O2−) were not affected (p > 0.05) by any GSH concentration. Interestingly, however, the addition of 8 mM or 10 mM GSH reduced (p < 0.05) the percentages of viable sperm with high overall ROS levels compared to the control. In conclusion, frozen-thawed donkey sperm are able to tolerate high GSH concentrations, which differs from what has been observed in other species. This antioxidant capacity suggests that ROS could be important during post-AI and that the impact of using exogenous antioxidants like GSH to improve the sperm resilience to freeze-thawing is limited in this speciesI.Y.-O. was funded by the Secretary of Higher Education, Science, Technology and Innovation (SENESCYT), Ecuador (Scheme: Programa de Becas Internacionales de Posgrado 2019; Grant: CZ02-000507-2019). J.C. was funded by the “Demetra” project of the Department of Excellence Postdoctoral fellowship, University of Teramo, Italy (Scheme: Assegno di Recerca 2021). The authors also acknowledge the support from the Ministry of Science and Innovation, Spain (Grant: AGL2017-88329-R) and the Regional Government of Catalonia (2017-SGR-1229MDPI (Multidisciplinary Digital Publishing Institute)Agencia Estatal de Investigación2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer-reviewedapplication/pdfhttp://hdl.handle.net/10256/20260http://hdl.handle.net/10256/20260Veterinary Sciences, 2021, vol. 8, núm. 12, p. 302Articles publicats (D-B)Yáñez-Ortiz, Iván Catalán, Jaime Delgado Bermúdez, Ariadna Carluccio, Augusto Miró, Jordi Yeste Oliveras, Marc 2021 Addition of reduced glutathione (GSH) to freezing medium reduces intracellular ROS levels in donkey sperm Veterinary Sciences 8 12 302reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.3390/vetsci8120302info:eu-repo/semantics/altIdentifier/eissn/2306-7381info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88329-RReconeixement 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessoai:recercat.cat:10256/202602026-05-29T05:05:01Z
dc.title.none.fl_str_mv Addition of reduced glutathione (GSH) to freezing medium reduces intracellular ROS levels in donkey sperm
title Addition of reduced glutathione (GSH) to freezing medium reduces intracellular ROS levels in donkey sperm
spellingShingle Addition of reduced glutathione (GSH) to freezing medium reduces intracellular ROS levels in donkey sperm
Yáñez-Ortiz, Iván
Ases -- Espermatozoides
Donkeys -- Spermatozoa
Espermatozoides
Spermatozoa
Semen -- Crioconservació
Semen -- Cryopreservation
title_short Addition of reduced glutathione (GSH) to freezing medium reduces intracellular ROS levels in donkey sperm
title_full Addition of reduced glutathione (GSH) to freezing medium reduces intracellular ROS levels in donkey sperm
title_fullStr Addition of reduced glutathione (GSH) to freezing medium reduces intracellular ROS levels in donkey sperm
title_full_unstemmed Addition of reduced glutathione (GSH) to freezing medium reduces intracellular ROS levels in donkey sperm
title_sort Addition of reduced glutathione (GSH) to freezing medium reduces intracellular ROS levels in donkey sperm
dc.creator.none.fl_str_mv Yáñez-Ortiz, Iván
Catalán, Jaime
Delgado Bermúdez, Ariadna
Carluccio, Augusto
Miró, Jordi
Yeste Oliveras, Marc
author Yáñez-Ortiz, Iván
author_facet Yáñez-Ortiz, Iván
Catalán, Jaime
Delgado Bermúdez, Ariadna
Carluccio, Augusto
Miró, Jordi
Yeste Oliveras, Marc
author_role author
author2 Catalán, Jaime
Delgado Bermúdez, Ariadna
Carluccio, Augusto
Miró, Jordi
Yeste Oliveras, Marc
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación
dc.subject.none.fl_str_mv Ases -- Espermatozoides
Donkeys -- Spermatozoa
Espermatozoides
Spermatozoa
Semen -- Crioconservació
Semen -- Cryopreservation
topic Ases -- Espermatozoides
Donkeys -- Spermatozoa
Espermatozoides
Spermatozoa
Semen -- Crioconservació
Semen -- Cryopreservation
description In donkeys, the use of frozen-thawed sperm for artificial insemination (AI) leads to low fertility rates. Furthermore, donkey sperm produce a large amount of reactive oxygen species (ROS), and post-AI inflammation induces the formation of neutrophil extracellular traps (NETosis), which further generates many more ROS. These high ROS levels may induce lipid peroxidation in the sperm plasma membrane, thus affecting its integrity. Enzymatic and non-enzymatic antioxidants, mainly found in the seminal plasma (SP), are responsible for maintaining the redox balance. However, this fluid is removed prior to cryopreservation, thereby exposing sperm cells to further oxidative stress. The exogenous addition of antioxidants to the freezing medium can reduce the detrimental effects caused by ROS generation. Therefore, the aim of this study was to evaluate how the addition of different reduced glutathione (GSH) concentrations (control, 2 mM, 4 mM, 6 mM, 8 mM, and 10 mM) to fresh sperm affect their cryotolerance. Total and progressive motility, kinematic parameters and motile sperm subpopulations were significantly (p < 0.05) different from the control in treatments containing 8 mM and 10 mM GSH, but not at lower concentrations. Plasma and acrosome membrane integrity, mitochondrial membrane potential (MMP) and intracellular superoxide levels (O2−) were not affected (p > 0.05) by any GSH concentration. Interestingly, however, the addition of 8 mM or 10 mM GSH reduced (p < 0.05) the percentages of viable sperm with high overall ROS levels compared to the control. In conclusion, frozen-thawed donkey sperm are able to tolerate high GSH concentrations, which differs from what has been observed in other species. This antioxidant capacity suggests that ROS could be important during post-AI and that the impact of using exogenous antioxidants like GSH to improve the sperm resilience to freeze-thawing is limited in this species
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
peer-reviewed
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/20260
http://hdl.handle.net/10256/20260
url http://hdl.handle.net/10256/20260
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/vetsci8120302
info:eu-repo/semantics/altIdentifier/eissn/2306-7381
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88329-R
dc.rights.none.fl_str_mv Reconeixement 4.0 Internacional
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Reconeixement 4.0 Internacional
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI (Multidisciplinary Digital Publishing Institute)
publisher.none.fl_str_mv MDPI (Multidisciplinary Digital Publishing Institute)
dc.source.none.fl_str_mv Veterinary Sciences, 2021, vol. 8, núm. 12, p. 302
Articles publicats (D-B)
Yáñez-Ortiz, Iván Catalán, Jaime Delgado Bermúdez, Ariadna Carluccio, Augusto Miró, Jordi Yeste Oliveras, Marc 2021 Addition of reduced glutathione (GSH) to freezing medium reduces intracellular ROS levels in donkey sperm Veterinary Sciences 8 12 302
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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