Reconstitution of respiratory complex I on a biomimetic membrane supported on gold electrodes
For the first time, respiratory complex I has been reconstituted on an electrode preserving its structure and activity. Respiratory complex I is a membrane-bound enzyme that has an essential function in cellular energy production. It couples NADH:quinone oxidoreduction to translocation of ions acros...
| Autores: | , , , , , , , |
|---|---|
| Formato: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2014 |
| País: | España |
| Recursos: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/142779 |
| Acesso em linha: | http://hdl.handle.net/10261/142779 |
| Access Level: | acceso abierto |
| Palavra-chave: | Infrared-absorption spectroscopy Nadh-Ubiquinone oxidoreductase Escherichia-coli Rhodothermus-marinus Lipid-membrane Cluster N2 Electrochemistry Oxidation Transport Bilayer Adenosinetriphosphate Atomic force microscopy Biomimetics Cell membranes Electrodes Gold Organic polymers Self assembled monolayers Electrochemical measurements Electrochemical potential Membrane-bound enzymes Mitochondrial membranes Proton translocation Respiratory complex Structure and activities Thiol self-assembled monolayers Gold compounds Artificial membranes Bacterial proteins Biomimetic materials Proton Reduced nicotinamide adenine dinucleotide dehydrogenase (ubiquinone) Thiol reagent Chemistry Electrode Electron transport Enzymology Isolation and purification Rhodothermus Electron Transport Complex I Membranes, Artificial Microscopy, Atomic Force Protons Sulfhydryl Reagents |
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Reconstitution of respiratory complex I on a biomimetic membrane supported on gold electrodesGutiérrez-Sanz, ÓscarOlea, DavidPita, MarcosBatista, Ana P.Alonso Caballero, ÁlvaroPereira, Manuela M.Vélez, MariselaLópez de Lacey, AntonioInfrared-absorption spectroscopyNadh-Ubiquinone oxidoreductaseEscherichia-coliRhodothermus-marinusLipid-membraneCluster N2ElectrochemistryOxidationTransportBilayerAdenosinetriphosphateAtomic force microscopyBiomimeticsCell membranesElectrodesGoldOrganic polymersSelf assembled monolayersElectrochemical measurementsElectrochemical potentialMembrane-bound enzymesMitochondrial membranesProton translocationRespiratory complexStructure and activitiesThiol self-assembled monolayersGold compoundsArtificial membranesBacterial proteinsBiomimetic materialsProtonAtomic force microscopyReduced nicotinamide adenine dinucleotide dehydrogenase (ubiquinone)Thiol reagentChemistryElectrodeElectron transportEnzymologyIsolation and purificationRhodothermusBacterial proteinsBiomimetic materialsElectrodesElectron transportElectron Transport Complex IMembranes, ArtificialMicroscopy, Atomic ForceProtonsRhodothermusSulfhydryl ReagentsFor the first time, respiratory complex I has been reconstituted on an electrode preserving its structure and activity. Respiratory complex I is a membrane-bound enzyme that has an essential function in cellular energy production. It couples NADH:quinone oxidoreduction to translocation of ions across the cellular (in prokaryotes) or mitochondrial membranes. Therefore, complex I contributes to the establishment and maintenance of the transmembrane difference of electrochemical potential required for adenosine triphosphate synthesis, transport, and motility. Our new strategy has been applied for reconstituting the bacterial complex I from Rhodothermus marinus onto a biomimetic membrane supported on gold electrodes modified with a thiol self-assembled monolayer (SAM). Atomic force microscopy and faradaic impedance measurements give evidence of the biomimetic construction, whereas electrochemical measurements show its functionality. Both electron transfer and proton translocation by respiratory complex I were monitored, simulating in vivo conditions. © 2014 American Chemical Society.This work was funded by the Spanish MINECO (project CTQ2012-32448) and by Fundação para a Ciência e a Tecnologia (PTDC/BBB-BQB/2294/2012 to M.M.P.). The work was also supported by Fundação para a Ciência e a Tecnologia through grant # PEst-OE/EQB/LA0004/2011. M.P. and O.G.-S. acknowledge the Ramon y Cajal and the FPI programs respectively from the Spanish MINECO. A.P.B. is recipient of a grant from Fundação para a Ciência e a Tecnologia (SFRH/BPD/80741/2011).Peer ReviewedAmerican Chemical SocietyMinisterio de Economía y Competitividad (España)Fundação para a Ciência e a Tecnologia (Portugal)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2017201720142017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/142779reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://doi.org/10.1021/la501825rSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1427792026-05-22T06:33:51Z |
| dc.title.none.fl_str_mv |
Reconstitution of respiratory complex I on a biomimetic membrane supported on gold electrodes |
| title |
Reconstitution of respiratory complex I on a biomimetic membrane supported on gold electrodes |
| spellingShingle |
Reconstitution of respiratory complex I on a biomimetic membrane supported on gold electrodes Gutiérrez-Sanz, Óscar Infrared-absorption spectroscopy Nadh-Ubiquinone oxidoreductase Escherichia-coli Rhodothermus-marinus Lipid-membrane Cluster N2 Electrochemistry Oxidation Transport Bilayer Adenosinetriphosphate Atomic force microscopy Biomimetics Cell membranes Electrodes Gold Organic polymers Self assembled monolayers Electrochemical measurements Electrochemical potential Membrane-bound enzymes Mitochondrial membranes Proton translocation Respiratory complex Structure and activities Thiol self-assembled monolayers Gold compounds Artificial membranes Bacterial proteins Biomimetic materials Proton Atomic force microscopy Reduced nicotinamide adenine dinucleotide dehydrogenase (ubiquinone) Thiol reagent Chemistry Electrode Electron transport Enzymology Isolation and purification Rhodothermus Bacterial proteins Biomimetic materials Electrodes Electron transport Electron Transport Complex I Membranes, Artificial Microscopy, Atomic Force Protons Rhodothermus Sulfhydryl Reagents |
| title_short |
Reconstitution of respiratory complex I on a biomimetic membrane supported on gold electrodes |
| title_full |
Reconstitution of respiratory complex I on a biomimetic membrane supported on gold electrodes |
| title_fullStr |
Reconstitution of respiratory complex I on a biomimetic membrane supported on gold electrodes |
| title_full_unstemmed |
Reconstitution of respiratory complex I on a biomimetic membrane supported on gold electrodes |
| title_sort |
Reconstitution of respiratory complex I on a biomimetic membrane supported on gold electrodes |
| dc.creator.none.fl_str_mv |
Gutiérrez-Sanz, Óscar Olea, David Pita, Marcos Batista, Ana P. Alonso Caballero, Álvaro Pereira, Manuela M. Vélez, Marisela López de Lacey, Antonio |
| author |
Gutiérrez-Sanz, Óscar |
| author_facet |
Gutiérrez-Sanz, Óscar Olea, David Pita, Marcos Batista, Ana P. Alonso Caballero, Álvaro Pereira, Manuela M. Vélez, Marisela López de Lacey, Antonio |
| author_role |
author |
| author2 |
Olea, David Pita, Marcos Batista, Ana P. Alonso Caballero, Álvaro Pereira, Manuela M. Vélez, Marisela López de Lacey, Antonio |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
Ministerio de Economía y Competitividad (España) Fundação para a Ciência e a Tecnologia (Portugal) Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
Infrared-absorption spectroscopy Nadh-Ubiquinone oxidoreductase Escherichia-coli Rhodothermus-marinus Lipid-membrane Cluster N2 Electrochemistry Oxidation Transport Bilayer Adenosinetriphosphate Atomic force microscopy Biomimetics Cell membranes Electrodes Gold Organic polymers Self assembled monolayers Electrochemical measurements Electrochemical potential Membrane-bound enzymes Mitochondrial membranes Proton translocation Respiratory complex Structure and activities Thiol self-assembled monolayers Gold compounds Artificial membranes Bacterial proteins Biomimetic materials Proton Atomic force microscopy Reduced nicotinamide adenine dinucleotide dehydrogenase (ubiquinone) Thiol reagent Chemistry Electrode Electron transport Enzymology Isolation and purification Rhodothermus Bacterial proteins Biomimetic materials Electrodes Electron transport Electron Transport Complex I Membranes, Artificial Microscopy, Atomic Force Protons Rhodothermus Sulfhydryl Reagents |
| topic |
Infrared-absorption spectroscopy Nadh-Ubiquinone oxidoreductase Escherichia-coli Rhodothermus-marinus Lipid-membrane Cluster N2 Electrochemistry Oxidation Transport Bilayer Adenosinetriphosphate Atomic force microscopy Biomimetics Cell membranes Electrodes Gold Organic polymers Self assembled monolayers Electrochemical measurements Electrochemical potential Membrane-bound enzymes Mitochondrial membranes Proton translocation Respiratory complex Structure and activities Thiol self-assembled monolayers Gold compounds Artificial membranes Bacterial proteins Biomimetic materials Proton Atomic force microscopy Reduced nicotinamide adenine dinucleotide dehydrogenase (ubiquinone) Thiol reagent Chemistry Electrode Electron transport Enzymology Isolation and purification Rhodothermus Bacterial proteins Biomimetic materials Electrodes Electron transport Electron Transport Complex I Membranes, Artificial Microscopy, Atomic Force Protons Rhodothermus Sulfhydryl Reagents |
| description |
For the first time, respiratory complex I has been reconstituted on an electrode preserving its structure and activity. Respiratory complex I is a membrane-bound enzyme that has an essential function in cellular energy production. It couples NADH:quinone oxidoreduction to translocation of ions across the cellular (in prokaryotes) or mitochondrial membranes. Therefore, complex I contributes to the establishment and maintenance of the transmembrane difference of electrochemical potential required for adenosine triphosphate synthesis, transport, and motility. Our new strategy has been applied for reconstituting the bacterial complex I from Rhodothermus marinus onto a biomimetic membrane supported on gold electrodes modified with a thiol self-assembled monolayer (SAM). Atomic force microscopy and faradaic impedance measurements give evidence of the biomimetic construction, whereas electrochemical measurements show its functionality. Both electron transfer and proton translocation by respiratory complex I were monitored, simulating in vivo conditions. © 2014 American Chemical Society. |
| publishDate |
2014 |
| dc.date.none.fl_str_mv |
2014 2017 2017 2017 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Postprint info:eu-repo/semantics/acceptedVersion |
| format |
article |
| status_str |
acceptedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10261/142779 |
| url |
http://hdl.handle.net/10261/142779 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
http://doi.org/10.1021/la501825r Sí |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
American Chemical Society |
| publisher.none.fl_str_mv |
American Chemical Society |
| dc.source.none.fl_str_mv |
reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
| instname_str |
Consejo Superior de Investigaciones Científicas (CSIC) |
| reponame_str |
DIGITAL.CSIC. Repositorio Institucional del CSIC |
| collection |
DIGITAL.CSIC. Repositorio Institucional del CSIC |
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1869406128724508672 |
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15,81155 |