Analysis of Human Natural Killer Cell Metabolism

Natural Killer (NK) cells mediate mainly innate anti-tumor and anti-viral immune responses and respond to a variety of cytokines and other stimuli to promote survival, cellular proliferation, production of cytokines such as interferon gamma (IFNγ) and/or cytotoxicity programs. NK cell activation by...

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Detalles Bibliográficos
Autores: Traba, Javier, Waldmann, Thomas, Antón Hurtado, Olga María
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/96932
Acceso en línea:https://hdl.handle.net/20.500.14352/96932
Access Level:acceso abierto
Palabra clave:577.1
Bioquímica (Biología)
2412.07 Inmunoquímica
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oai_identifier_str oai:docta.ucm.es:20.500.14352/96932
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spelling Analysis of Human Natural Killer Cell MetabolismTraba, JavierWaldmann, ThomasAntón Hurtado, Olga María577.1Bioquímica (Biología)2412.07 InmunoquímicaNatural Killer (NK) cells mediate mainly innate anti-tumor and anti-viral immune responses and respond to a variety of cytokines and other stimuli to promote survival, cellular proliferation, production of cytokines such as interferon gamma (IFNγ) and/or cytotoxicity programs. NK cell activation by cytokine stimulation requires a substantial remodeling of metabolic pathways to support their bioenergetic and biosynthetic requirements. There is a large body of evidence that suggests that impaired NK cell metabolism is associated with a number of chronic diseases including obesity and cancer, which highlights the clinical importance of the availability of a method to determine NK cell metabolism. Here we describe the use of an extracellular flux analyzer, a platform that allows real-time measurements of glycolysis and mitochondrial oxygen consumption, as a tool to monitor changes in the energy metabolism of human NK cells. The method described here also allows for the monitoring of metabolic changes after stimulation of NK cells with cytokines such as IL-15, a system that is currently being investigated in a wide range of clinical trials.My Jove CorporationUniversidad Complutense de Madrid20202020-01-0120202020-01-01journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/96932reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/969322026-06-02T12:44:21Z
dc.title.none.fl_str_mv Analysis of Human Natural Killer Cell Metabolism
title Analysis of Human Natural Killer Cell Metabolism
spellingShingle Analysis of Human Natural Killer Cell Metabolism
Traba, Javier
577.1
Bioquímica (Biología)
2412.07 Inmunoquímica
title_short Analysis of Human Natural Killer Cell Metabolism
title_full Analysis of Human Natural Killer Cell Metabolism
title_fullStr Analysis of Human Natural Killer Cell Metabolism
title_full_unstemmed Analysis of Human Natural Killer Cell Metabolism
title_sort Analysis of Human Natural Killer Cell Metabolism
dc.creator.none.fl_str_mv Traba, Javier
Waldmann, Thomas
Antón Hurtado, Olga María
author Traba, Javier
author_facet Traba, Javier
Waldmann, Thomas
Antón Hurtado, Olga María
author_role author
author2 Waldmann, Thomas
Antón Hurtado, Olga María
author2_role author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 577.1
Bioquímica (Biología)
2412.07 Inmunoquímica
topic 577.1
Bioquímica (Biología)
2412.07 Inmunoquímica
description Natural Killer (NK) cells mediate mainly innate anti-tumor and anti-viral immune responses and respond to a variety of cytokines and other stimuli to promote survival, cellular proliferation, production of cytokines such as interferon gamma (IFNγ) and/or cytotoxicity programs. NK cell activation by cytokine stimulation requires a substantial remodeling of metabolic pathways to support their bioenergetic and biosynthetic requirements. There is a large body of evidence that suggests that impaired NK cell metabolism is associated with a number of chronic diseases including obesity and cancer, which highlights the clinical importance of the availability of a method to determine NK cell metabolism. Here we describe the use of an extracellular flux analyzer, a platform that allows real-time measurements of glycolysis and mitochondrial oxygen consumption, as a tool to monitor changes in the energy metabolism of human NK cells. The method described here also allows for the monitoring of metabolic changes after stimulation of NK cells with cytokines such as IL-15, a system that is currently being investigated in a wide range of clinical trials.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01
2020
2020-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/96932
url https://hdl.handle.net/20.500.14352/96932
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv My Jove Corporation
publisher.none.fl_str_mv My Jove Corporation
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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